Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Studies on the amphibian neuromuscular junction have indicated that N-type calcium channels are the sole mediators of stimulus-evoked neurotransmitter release. We show, via both presynaptic and postsynaptic voltage-clamp measurements, that dihydropyridine (DHP)-sensitive calcium channels also contribute to stimulus-evoked release at developing Xenopus neuromuscular junctions. Whereas inhibition of postsynaptic responses by omega-conotoxin (omega-Ctx) GVIA has been taken previously as evidence that only N-type channels mediate transmitter release, we find that both N-type and DHP-sensitive calcium currents are sensitive to this toxin. The unusual sensitivity of DHP-sensitive calcium channels to omega-Ctx GVIA in presynaptic terminals raises the possibility that this channel type may have escaped detection in previous physiological studies on adult frog neuromuscular junctions. Alternatively, the additional channel isoforms may be present only during early development, when they may serve to strengthen collectively presynaptic release during critical periods of synaptogenesis.
Anderson,
Correlation between acetylcholine receptor localization and spontaneous synaptic potentials in cultures of nerve and muscle.
1979, Pubmed,
Xenbase
Anderson,
Correlation between acetylcholine receptor localization and spontaneous synaptic potentials in cultures of nerve and muscle.
1979,
Pubmed
,
Xenbase
Anderson,
Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells.
1977,
Pubmed
,
Xenbase
Aosaki,
Characterization of two kinds of high-voltage-activated Ca-channel currents in chick sensory neurons. Differential sensitivity to dihydropyridines and omega-conotoxin GVIA.
1989,
Pubmed
Barry,
Liquid junction potentials and small cell effects in patch-clamp analysis.
1991,
Pubmed
Borst,
Pre- and postsynaptic whole-cell recordings in the medial nucleus of the trapezoid body of the rat.
1995,
Pubmed
Brehm,
Acetylcholine receptor channel properties during development of Xenopus muscle cells in culture.
1984,
Pubmed
,
Xenbase
Brehm,
Properties of non-junctional acetylcholine receptor channels on innervated muscle of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Buchanan,
Studies of nerve-muscle interactions in Xenopus cell culture: fine structure of early functional contacts.
1989,
Pubmed
,
Xenbase
Butz,
A tripartite protein complex with the potential to couple synaptic vesicle exocytosis to cell adhesion in brain.
1998,
Pubmed
Byerly,
Calcium currents in internally perfused nerve cell bodies of Limnea stagnalis.
1982,
Pubmed
Cruz,
Calcium channel antagonists. Omega-conotoxin defines a new high affinity site.
1986,
Pubmed
Day,
Differential localization of voltage-dependent calcium channel alpha1 subunits at the human and rat neuromuscular junction.
1997,
Pubmed
DEL CASTILLO,
Localization of active spots within the neuromuscular junction of the frog.
1956,
Pubmed
Dodge,
Co-operative action a calcium ions in transmitter release at the neuromuscular junction.
1967,
Pubmed
Dunlap,
Exocytotic Ca2+ channels in mammalian central neurons.
1995,
Pubmed
Ellinor,
Functional expression of a rapidly inactivating neuronal calcium channel.
1993,
Pubmed
,
Xenbase
Fox,
Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurones.
1987,
Pubmed
Gray,
Developmental switch in the pharmacology of Ca2+ channels coupled to acetylcholine release.
1992,
Pubmed
Hartsel,
Na+, K+ and Cl- selectivity of the permeability pathways induced through sterol-containing membrane vesicles by amphotericin B and other polyene antibiotics.
1994,
Pubmed
Hillyard,
A new Conus peptide ligand for mammalian presynaptic Ca2+ channels.
1992,
Pubmed
Hirning,
Dominant role of N-type Ca2+ channels in evoked release of norepinephrine from sympathetic neurons.
1988,
Pubmed
Horn,
Muscarinic activation of ionic currents measured by a new whole-cell recording method.
1988,
Pubmed
Hulsizer,
Calcium currents in presynaptic varicosities of embryonic motoneurons.
1991,
Pubmed
,
Xenbase
Iwasaki,
Developmental changes in calcium channel types mediating central synaptic transmission.
2000,
Pubmed
Iwasaki,
Developmental changes in calcium channel types mediating synaptic transmission in rat auditory brainstem.
1998,
Pubmed
Kasai,
Dihydropyridine-sensitive and omega-conotoxin-sensitive calcium channels in a mammalian neuroblastoma-glioma cell line.
1992,
Pubmed
Kasai,
Presynaptic Ca-antagonist omega-conotoxin irreversibly blocks N-type Ca-channels in chick sensory neurons.
1987,
Pubmed
Katz,
Effects of Ca2+ channel blockers on transmitter release and presynaptic currents at the frog neuromuscular junction.
1995,
Pubmed
Katz,
The timing of calcium action during neuromuscular transmission.
1967,
Pubmed
Kerr,
A venom peptide with a novel presynaptic blocking action.
,
Pubmed
Kidokoro,
Redistribution of acetylcholine receptors during neuromuscular junction formation in Xenopus cultures.
1985,
Pubmed
,
Xenbase
Kidokoro,
Changes in synaptic potential properties during acetylcholine receptor accumulation and neurospecific interactions in Xenopus nerve-muscle cell culture.
1980,
Pubmed
,
Xenbase
Kostyuk,
Effects of calcium and calcium-chelating agents on the inward and outward current in the membrane of mollusc neurones.
1977,
Pubmed
Koyano,
Effects of synthetic omega-conotoxin on synaptic transmission.
1987,
Pubmed
Kullberg,
Development of the myotomal neuromuscular junction in Xenopus laevis: an electrophysiological and fine-structural study.
1977,
Pubmed
,
Xenbase
Lévêque,
Purification of the N-type calcium channel associated with syntaxin and synaptotagmin. A complex implicated in synaptic vesicle exocytosis.
1994,
Pubmed
Lindgren,
Identification of ionic currents at presynaptic nerve endings of the lizard.
1989,
Pubmed
Llinás,
Blocking and isolation of a calcium channel from neurons in mammals and cephalopods utilizing a toxin fraction (FTX) from funnel-web spider poison.
1989,
Pubmed
Llinás,
Relationship between presynaptic calcium current and postsynaptic potential in squid giant synapse.
1981,
Pubmed
Luebke,
Multiple calcium channel types control glutamatergic synaptic transmission in the hippocampus.
1993,
Pubmed
McCleskey,
Omega-conotoxin: direct and persistent blockade of specific types of calcium channels in neurons but not muscle.
1987,
Pubmed
McDonough,
Inhibition of calcium channels in rat central and peripheral neurons by omega-conotoxin MVIIC.
1996,
Pubmed
Mynlieff,
Characterization of voltage-dependent calcium currents in mouse motoneurons.
1992,
Pubmed
Neher,
Correction for liquid junction potentials in patch clamp experiments.
1992,
Pubmed
Nowycky,
Three types of neuronal calcium channel with different calcium agonist sensitivity.
,
Pubmed
Olivera,
Purification and sequence of a presynaptic peptide toxin from Conus geographus venom.
1984,
Pubmed
Olivera,
Peptide neurotoxins from fish-hunting cone snails.
1985,
Pubmed
Peng,
Development of the postsynaptic membrane in Xenopus neuromuscular cultures observed by freeze-fracture and thin-section electron microscopy.
1980,
Pubmed
,
Xenbase
Plummer,
Elementary properties and pharmacological sensitivities of calcium channels in mammalian peripheral neurons.
1989,
Pubmed
Protti,
Calcium channel blockers and transmitter release at the normal human neuromuscular junction.
1996,
Pubmed
Randall,
Pharmacological dissection of multiple types of Ca2+ channel currents in rat cerebellar granule neurons.
1995,
Pubmed
,
Xenbase
Regan,
Ca2+ channels in rat central and peripheral neurons: high-threshold current resistant to dihydropyridine blockers and omega-conotoxin.
1991,
Pubmed
Regehr,
Participation of multiple calcium channel types in transmission at single climbing fiber to Purkinje cell synapses.
1994,
Pubmed
Robitaille,
Strategic location of calcium channels at transmitter release sites of frog neuromuscular synapses.
1990,
Pubmed
Robitaille,
Localization of L-type Ca2+ channels at perisynaptic glial cells of the frog neuromuscular junction.
1996,
Pubmed
Rosato Siri,
Calcium channels coupled to neurotransmitter release at neonatal rat neuromuscular junctions.
1999,
Pubmed
Sano,
Effects of synthetic omega-conotoxin, a new type Ca2+ antagonist, on frog and mouse neuromuscular transmission.
1987,
Pubmed
Sheng,
Identification of a syntaxin-binding site on N-type calcium channels.
1994,
Pubmed
Stanley,
Characterization of a calcium current in a vertebrate cholinergic presynaptic nerve terminal.
1991,
Pubmed
Takahashi,
Structure and physiology of developing neuromuscular synapses in culture.
1987,
Pubmed
,
Xenbase
Torri Tarelli,
Presynaptic localization of omega-conotoxin-sensitive calcium channels at the frog neuromuscular junction.
1991,
Pubmed
Toselli,
Pharmacological characterization of voltage-dependent calcium currents in rat hippocampal neurons.
1990,
Pubmed
Turner,
Multiple Ca2+ channel types coexist to regulate synaptosomal neurotransmitter release.
1993,
Pubmed
Wang,
Two types of high-threshold calcium currents inhibited by omega-conotoxin in nerve terminals of rat neurohypophysis.
1992,
Pubmed
Westenbroek,
Localization of Ca2+ channel subtypes on rat spinal motor neurons, interneurons, and nerve terminals.
1998,
Pubmed
Williams,
Structure and functional expression of alpha 1, alpha 2, and beta subunits of a novel human neuronal calcium channel subtype.
1992,
Pubmed
,
Xenbase
Yawo,
Re-evaluation of calcium currents in pre- and postsynaptic neurones of the chick ciliary ganglion.
1993,
Pubmed
Yazejian,
Direct measurements of presynaptic calcium and calcium-activated potassium currents regulating neurotransmitter release at cultured Xenopus nerve-muscle synapses.
1997,
Pubmed
,
Xenbase
