Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Dev Biol
2007 Jun 01;3061:121-33. doi: 10.1016/j.ydbio.2007.03.011.
Show Gene links
Show Anatomy links
Wnt5a functions in planar cell polarity regulation in mice.
Qian D
,
Jones C
,
Rzadzinska A
,
Mark S
,
Zhang X
,
Steel KP
,
Dai X
,
Chen P
.
???displayArticle.abstract???
Planar cell polarity (PCP) refers to the polarization of cells within the plane of a cell sheet. A distinctive epithelial PCP in vertebrates is the uniform orientation of stereociliary bundles of the sensory hair cells in the mammalian cochlea. In addition to establishing epithelial PCP, planar polarization is also required for convergent extension (CE); a polarized cellular movement that occurs during neural tube closure and cochlear extension. Studies in Drosophila and vertebrates have revealed a conserved PCP pathway, including Frizzled (Fz) receptors. Here we use the cochlea as a model system to explore the involvement of known ligands of Fz, Wnt morphogens, in PCP regulation. We show that Wnt5a forms a reciprocal expression pattern with a Wnt antagonist, the secreted frizzled-related protein 3 (Sfrp3 or Frzb), along the axis of planar polarization in the cochlear epithelium. We further demonstrate that Wnt5a antagonizes Frzb in regulating cochlear extension and stereociliary bundle orientation in vitro, and that Wnt5a(-/-) animals have a shortened and widened cochlea. Finally, we show that Wnt5a is required for proper subcellular distribution of a PCP protein, Ltap/Vangl2, and that Wnt5a interacts genetically with Ltap/Vangl2 for uniform orientation of stereocilia, cochlear extension, and neural tube closure. Together, these findings demonstrate that Wnt5a functions in PCP regulation in mice.
Amonlirdviman,
Mathematical modeling of planar cell polarity to understand domineering nonautonomy.
2005, Pubmed
Amonlirdviman,
Mathematical modeling of planar cell polarity to understand domineering nonautonomy.
2005,
Pubmed
Badea,
A noninvasive genetic/pharmacologic strategy for visualizing cell morphology and clonal relationships in the mouse.
2003,
Pubmed
Bhanot,
A new member of the frizzled family from Drosophila functions as a Wingless receptor.
1996,
Pubmed
Blankenship,
Multicellular rosette formation links planar cell polarity to tissue morphogenesis.
2006,
Pubmed
Chen,
p27(Kip1) links cell proliferation to morphogenesis in the developing organ of Corti.
1999,
Pubmed
Chen,
The role of Math1 in inner ear development: Uncoupling the establishment of the sensory primordium from hair cell fate determination.
2002,
Pubmed
Ciruna,
Planar cell polarity signalling couples cell division and morphogenesis during neurulation.
2006,
Pubmed
Classen,
Hexagonal packing of Drosophila wing epithelial cells by the planar cell polarity pathway.
2005,
Pubmed
Colosimo,
Wnt, Hedgehog and junctional Armadillo/beta-catenin establish planar polarity in the Drosophila embryo.
2006,
Pubmed
Curtin,
Mutation of Celsr1 disrupts planar polarity of inner ear hair cells and causes severe neural tube defects in the mouse.
2003,
Pubmed
Dabdoub,
Wnt signaling mediates reorientation of outer hair cell stereociliary bundles in the mammalian cochlea.
2003,
Pubmed
Davies,
Planar polarity of hair cells in the chick inner ear is correlated with polarized distribution of c-flamingo-1 protein.
2005,
Pubmed
Gubb,
A genetic analysis of the determination of cuticular polarity during development in Drosophila melanogaster.
1982,
Pubmed
Heisenberg,
Silberblick/Wnt11 mediates convergent extension movements during zebrafish gastrulation.
2000,
Pubmed
Hyodo-Miura,
XGAP, an ArfGAP, is required for polarized localization of PAR proteins and cell polarity in Xenopus gastrulation.
2006,
Pubmed
,
Xenbase
Jiang,
Ascidian prickle regulates both mediolateral and anterior-posterior cell polarity of notochord cells.
2005,
Pubmed
Keller,
Shaping the vertebrate body plan by polarized embryonic cell movements.
2002,
Pubmed
Kibar,
Ltap, a mammalian homolog of Drosophila Strabismus/Van Gogh, is altered in the mouse neural tube mutant Loop-tail.
2001,
Pubmed
Kilian,
The role of Ppt/Wnt5 in regulating cell shape and movement during zebrafish gastrulation.
2003,
Pubmed
Klein,
Planar cell polarization: an emerging model points in the right direction.
2005,
Pubmed
Lee,
Embryonic dorsal-ventral signaling: secreted frizzled-related proteins as inhibitors of tolloid proteinases.
2006,
Pubmed
,
Xenbase
Liu,
The mechanism of endogenous receptor activation functionally distinguishes prototype canonical and noncanonical Wnts.
2005,
Pubmed
Logan,
The Wnt signaling pathway in development and disease.
2004,
Pubmed
Lu,
PTK7/CCK-4 is a novel regulator of planar cell polarity in vertebrates.
2004,
Pubmed
,
Xenbase
Lumpkin,
Math1-driven GFP expression in the developing nervous system of transgenic mice.
2003,
Pubmed
Maretto,
Mapping Wnt/beta-catenin signaling during mouse development and in colorectal tumors.
2003,
Pubmed
McKenzie,
Cellular growth and rearrangement during the development of the mammalian organ of Corti.
2004,
Pubmed
Mikels,
Purified Wnt5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context.
2006,
Pubmed
Mlodzik,
A GAP in convergent extension scores PAR.
2006,
Pubmed
,
Xenbase
Mlodzik,
Planar cell polarization: do the same mechanisms regulate Drosophila tissue polarity and vertebrate gastrulation?
2002,
Pubmed
Moeller,
Diversin regulates heart formation and gastrulation movements in development.
2006,
Pubmed
Montcouquiol,
Identification of Vangl2 and Scrb1 as planar polarity genes in mammals.
2003,
Pubmed
Montcouquiol,
Asymmetric localization of Vangl2 and Fz3 indicate novel mechanisms for planar cell polarity in mammals.
2006,
Pubmed
Morsli,
Development of the mouse inner ear and origin of its sensory organs.
1998,
Pubmed
Muraoka,
Sizzled controls dorso-ventral polarity by repressing cleavage of the Chordin protein.
2006,
Pubmed
Price,
Cytoskeletal dynamics and cell signaling during planar polarity establishment in the Drosophila embryonic denticle.
2006,
Pubmed
Radde-Gallwitz,
Expression of Islet1 marks the sensory and neuronal lineages in the mammalian inner ear.
2004,
Pubmed
Rattner,
A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors.
1997,
Pubmed
Ruben,
Development of the inner ear of the mouse: a radioautographic study of terminal mitoses.
1967,
Pubmed
Schwarz-Romond,
The ankyrin repeat protein Diversin recruits Casein kinase Iepsilon to the beta-catenin degradation complex and acts in both canonical Wnt and Wnt/JNK signaling.
2002,
Pubmed
,
Xenbase
Simons,
Inversin, the gene product mutated in nephronophthisis type II, functions as a molecular switch between Wnt signaling pathways.
2005,
Pubmed
,
Xenbase
Smith,
Xwnt11 and the regulation of gastrulation in Xenopus.
2000,
Pubmed
,
Xenbase
Strutt,
Long-range coordination of planar polarity in Drosophila.
2005,
Pubmed
Tada,
Xwnt11 is a target of Xenopus Brachyury: regulation of gastrulation movements via Dishevelled, but not through the canonical Wnt pathway.
2000,
Pubmed
,
Xenbase
Takemura,
Localization of bone morphogenetic protein-4 messenger RNA in developing mouse cochlea.
1996,
Pubmed
Topczewski,
The zebrafish glypican knypek controls cell polarity during gastrulation movements of convergent extension.
2001,
Pubmed
Tree,
A three-tiered mechanism for regulation of planar cell polarity.
2002,
Pubmed
Ulrich,
Slb/Wnt11 controls hypoblast cell migration and morphogenesis at the onset of zebrafish gastrulation.
2003,
Pubmed
Vinson,
A Drosophila tissue polarity locus encodes a protein containing seven potential transmembrane domains.
1989,
Pubmed
Wallingford,
Dishevelled controls cell polarity during Xenopus gastrulation.
2000,
Pubmed
,
Xenbase
Wang,
Frzb, a secreted protein expressed in the Spemann organizer, binds and inhibits Wnt-8.
1997,
Pubmed
,
Xenbase
Wang,
Regulation of polarized extension and planar cell polarity in the cochlea by the vertebrate PCP pathway.
2005,
Pubmed
Wang,
The role of Frizzled3 and Frizzled6 in neural tube closure and in the planar polarity of inner-ear sensory hair cells.
2006,
Pubmed
Wang,
Dishevelled genes mediate a conserved mammalian PCP pathway to regulate convergent extension during neurulation.
2006,
Pubmed
,
Xenbase
Yamaguchi,
A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo.
1999,
Pubmed
Yang,
Homologous recombination based modification in Escherichia coli and germline transmission in transgenic mice of a bacterial artificial chromosome.
1997,
Pubmed
Yang-Snyder,
A frizzled homolog functions in a vertebrate Wnt signaling pathway.
1996,
Pubmed
,
Xenbase
