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EMBO J
1991 Nov 01;1011:3239-45. doi: 10.1002/j.1460-2075.1991.tb04887.x.
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Sequence of a functional invertebrate GABAA receptor subunit which can form a chimeric receptor with a vertebrate alpha subunit.
Harvey RJ
,
Vreugdenhil E
,
Zaman SH
,
Bhandal NS
,
Usherwood PN
,
Barnard EA
,
Darlison MG
.
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The sequence of an invertebrate GABAA receptor subunit is described. This was deduced from a cDNA which was isolated from the mollusc Lymnaea stagnalis and which corresponds to a transcript of extremely low abundance. The cDNA was isolated using short exonic sequences from part of the corresponding gene in combination with a variant of the polymerase chain reaction (PCR) known as RACE (rapid amplification of cDNA ends). The mature polypeptide has a predicted molecular weight of 54,569 Daltons and exhibits approximately 50% identity to vertebrate GABAA receptor beta subunits. The six intron-exon boundaries determined to date in the molluscan gene occur at the same relative positions as those found in vertebrate GABAA receptor genes. Functional expression, in Xenopus oocytes, of the molluscan cDNA alone results in the formation of GABA-activated chloride ion channels that have a finite open probability even in the absence of agonist. These GABA-evoked currents can be reversibly blocked by the vertebrate GABAA receptor antagonist bicuculline. Surprisingly, the molluscan beta subunit is capable of replacing vertebrate beta subunits in co-expression experiments with the bovine GABAA receptor alpha 1 subunit. These findings suggest that invertebrate GABAA receptors exist in vivo as hetero-oligomeric complexes.
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Barton,
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Bateson,
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Benton,
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1977,
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Blair,
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1988,
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,
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Bormann,
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1987,
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,
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Frohman,
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1988,
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,
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Grenningloh,
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,
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,
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Isacoff,
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,
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Jansen,
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Kemp,
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1989,
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,
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Kornfeld,
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1985,
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Kuhse,
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,
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Lasham,
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Levitan,
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1988,
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,
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Lüddens,
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Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Norrander,
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
1983,
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Olsen,
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Pritchett,
Importance of a novel GABAA receptor subunit for benzodiazepine pharmacology.
1989,
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Pritchett,
Gamma-aminobutyric acidA receptor alpha 5-subunit creates novel type II benzodiazepine receptor pharmacology.
1990,
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Pritchett,
Type I and type II GABAA-benzodiazepine receptors produced in transfected cells.
1989,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sattelle,
GABA receptors on the cell-body membrane of an identified insect motor neuron.
1988,
Pubmed
Schofield,
Sequence and functional expression of the GABA A receptor shows a ligand-gated receptor super-family.
,
Pubmed
,
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Shivers,
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1989,
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Sigel,
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1989,
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,
Xenbase
Sigel,
The effect of subunit composition of rat brain GABAA receptors on channel function.
1990,
Pubmed
,
Xenbase
Smith,
Alternative splicing in the control of gene expression.
1989,
Pubmed
Sommer,
The murine GABAA receptor delta-subunit gene: structure and assignment to human chromosome 1.
1990,
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Syed,
In vitro reconstruction of the respiratory central pattern generator of the mollusk Lymnaea.
1990,
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Verdoorn,
Functional properties of recombinant rat GABAA receptors depend upon subunit composition.
1990,
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von Heijne,
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1986,
Pubmed
Vreugdenhil,
Isolation, characterization, and evolutionary aspects of a cDNA clone encoding multiple neuropeptides involved in the stereotyped egg-laying behavior of the freshwater snail Lymnaea stagnalis.
1988,
Pubmed
White,
Mouse-Torpedo hybrid acetylcholine receptors: functional homology does not equal sequence homology.
1985,
Pubmed
,
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Wilson-Shaw,
A novel gamma subunit of the GABAA receptor identified using the polymerase chain reaction.
1991,
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Wisden,
Distinct GABAA receptor alpha subunit mRNAs show differential patterns of expression in bovine brain.
1988,
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Wisden,
Localization of GABAA receptor alpha-subunit mRNAs in relation to receptor subtypes.
1989,
Pubmed
Yarowsky,
Receptors for gamma-aminobutyric acid (GABA) on Aplysia neurons.
1978,
Pubmed
Ymer,
Sequence and expression of a novel GABAA receptor alpha subunit.
1989,
Pubmed
,
Xenbase
Ymer,
GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.
1989,
Pubmed
,
Xenbase
Ymer,
Structural and functional characterization of the gamma 1 subunit of GABAA/benzodiazepine receptors.
1990,
Pubmed
,
Xenbase
