Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Genetics
2002 Nov 01;1623:1211-20. doi: 10.1093/genetics/162.3.1211.
Show Gene links
Show Anatomy links
The gene for the intermediate chain subunit of cytoplasmic dynein is essential in Drosophila.
Boylan KL
,
Hays TS
.
???displayArticle.abstract???
The microtubule motor cytoplasmic dynein powers a variety of intracellular transport events that are essential for cellular and developmental processes. A current hypothesis is that the accessory subunits of the dynein complex are important for the specialization of cytoplasmic dynein function. In a genetic approach to understanding the range of dynein functions and the contribution of the different subunits to dynein motor function and regulation, we have identified mutations in the gene for the cytoplasmic dynein intermediate chain, Dic19C. We used a functional Dic transgene in a genetic screen to recover X-linked lethal mutations that require this transgene for viability. Three Dic mutations were identified and characterized. All three Dic alleles result in larval lethality, demonstrating that the intermediate chain serves an essential function in Drosophila. Like a deficiency that removes Dic19C, the Dic mutations dominantly enhance the rough eye phenotype of Glued(1), a dominant mutation in the gene for the p150 subunit of the dynactin complex, a dynein activator. Additionally, we used complementation analysis to identify an existing mutation, shortwing (sw), as an allele of the dynein intermediate chain gene. Unlike the Dic alleles isolated de novo, shortwing is homozygous viable and exhibits recessive and temperature-sensitive defects in eye and wing development. These phenotypes are rescued by the wild-type Dic transgene, indicating that shortwing is a viable allele of the dynein intermediate chain gene and revealing a novel role for dynein function during wing development.
Bowman,
Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis.
1999, Pubmed
Bowman,
Drosophila roadblock and Chlamydomonas LC7: a conserved family of dynein-associated proteins involved in axonal transport, flagellar motility, and mitosis.
1999,
Pubmed
Boylan,
A molecular genetic analysis of the interaction between the cytoplasmic dynein intermediate chain and the glued (dynactin) complex.
2000,
Pubmed
Carthew,
seven in absentia, a gene required for specification of R7 cell fate in the Drosophila eye.
1990,
Pubmed
Chuang,
Subunit heterogeneity of cytoplasmic dynein: Differential expression of 14 kDa dynein light chains in rat hippocampus.
2001,
Pubmed
Dillman,
Differential phosphorylation in vivo of cytoplasmic dynein associated with anterogradely moving organelles.
1994,
Pubmed
Echeverri,
Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis.
1996,
Pubmed
Eshel,
Cytoplasmic dynein is required for normal nuclear segregation in yeast.
1993,
Pubmed
Fehon,
Isolation of mutations in the Drosophila homologues of the human Neurofibromatosis 2 and yeast CDC42 genes using a simple and efficient reverse-genetic method.
1997,
Pubmed
Geiser,
Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways.
1997,
Pubmed
Gepner,
Cytoplasmic dynein function is essential in Drosophila melanogaster.
1996,
Pubmed
Gindhart,
Kinesin light chains are essential for axonal transport in Drosophila.
1998,
Pubmed
Harte,
Genetic analysis of mutations at the Glued locus and interacting loci in Drosophila melanogaster.
1982,
Pubmed
Holleran,
Centractin (ARP1) associates with spectrin revealing a potential mechanism to link dynactin to intracellular organelles.
1996,
Pubmed
Holzbaur,
DYNEINS: molecular structure and cellular function.
1994,
Pubmed
Hurd,
Kinesin mutations cause motor neuron disease phenotypes by disrupting fast axonal transport in Drosophila.
1996,
Pubmed
Karki,
Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex.
1995,
Pubmed
Karki,
Cytoplasmic dynein and dynactin in cell division and intracellular transport.
1999,
Pubmed
King,
Localization of an intermediate chain of outer arm dynein by immunoelectron microscopy.
1990,
Pubmed
King,
Dynactin increases the processivity of the cytoplasmic dynein motor.
2000,
Pubmed
King,
The Mr 78,000 intermediate chain of Chlamydomonas outer arm dynein interacts with alpha-tubulin in situ.
1991,
Pubmed
Koonce,
Cytoplasmic dynein heavy chain is an essential gene product in Dictyostelium.
1998,
Pubmed
Lefevre,
The distribution of randomly recovered X-ray-induced sex-linked genetic effects in Drosophila melanogaster.
1981,
Pubmed
Lefevre,
Genetic duplication in the white-split interval of the X chromosome in Drosophila melanogaster.
1972,
Pubmed
Li,
Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte.
1994,
Pubmed
Li,
Disruption of mitotic spindle orientation in a yeast dynein mutant.
1993,
Pubmed
Martin,
Cytoplasmic dynein, the dynactin complex, and kinesin are interdependent and essential for fast axonal transport.
1999,
Pubmed
McGrail,
Regulation of cytoplasmic dynein function in vivo by the Drosophila Glued complex.
1995,
Pubmed
McGrail,
The microtubule motor cytoplasmic dynein is required for spindle orientation during germline cell divisions and oocyte differentiation in Drosophila.
1997,
Pubmed
Nurminsky,
Cytoplasmic dynein intermediate-chain isoforms with different targeting properties created by tissue-specific alternative splicing.
1998,
Pubmed
Parádi,
Effect of storage and dose on MMS-induced deletions. Complementation analysis of X-chromosomal recessive lethals in the zeste-white and maroon-like regions of Drosophila melanogaster.
1983,
Pubmed
Paschal,
Homology of the 74-kD cytoplasmic dynein subunit with a flagellar dynein polypeptide suggests an intracellular targeting function.
1992,
Pubmed
Pfister,
Differential expression and phosphorylation of the 74-kDa intermediate chains of cytoplasmic dynein in cultured neurons and glia.
1996,
Pubmed
Plamann,
Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi.
1994,
Pubmed
Schroer,
Two activators of microtubule-based vesicle transport.
1991,
Pubmed
Simmons,
High mutability in male hybrids of Drosophila melanogaster.
1980,
Pubmed
Steffen,
Immunogold localisation of the intermediate chain within the protein complex of cytoplasmic dynein.
1996,
Pubmed
Swaroop,
Molecular genetics of a transposon-induced dominant mutation in the Drosophila locus Glued.
1985,
Pubmed
Tai,
Localization of Tctex-1, a cytoplasmic dynein light chain, to the Golgi apparatus and evidence for dynein complex heterogeneity.
1998,
Pubmed
Tai,
Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport.
2001,
Pubmed
Tai,
Rhodopsin's carboxy-terminal cytoplasmic tail acts as a membrane receptor for cytoplasmic dynein by binding to the dynein light chain Tctex-1.
1999,
Pubmed
Tynan,
Light intermediate chain 1 defines a functional subfraction of cytoplasmic dynein which binds to pericentrin.
2000,
Pubmed
Vallee,
Microtubule-associated protein 1C from brain is a two-headed cytosolic dynein.
1988,
Pubmed
Zinsmaier,
Paralysis and early death in cysteine string protein mutants of Drosophila.
1994,
Pubmed
