XB-ART-43115
Dev Genes Evol
2011 May 01;2211:29-41. doi: 10.1007/s00427-011-0357-5.
Show Gene links
Show Anatomy links
Characterization of Cer-1 cis-regulatory region during early Xenopus development.
???displayArticle.abstract???
Cerberus-related molecules are well-known Wnt, Nodal, and BMP inhibitors that have been implicated in different processes including anterior–posterior patterning and left–right asymmetry. In both mouse and frog, two Cerberus-related genes have been isolated, mCer-1 and mCer-2, and Xcer and Xcoco, respectively. Until now, little is known about the mechanisms involved in their transcriptional regulation. Here, we report a heterologous analysis of the mouse Cerberus-1 gene upstream regulatory regions, responsible for its expression in the visceral endodermal cells. Our analysis showed that the consensus sequences for a TATA, CAAT, or GC boxes were absent but a TGTGG sequence was present at position -172 to -168 bp, relative to the ATG. Using a series of deletion constructs and transient expression in Xenopus embryos, we found that a fragment of 1.4 kb of Cer-1 promoter sequence could reproduce the endogenous expression pattern of Xenopus cerberus. A 0.7-kb mcer-1 upstream region was able to drive reporter expression to the involuting mesendodermal cells, while further deletions abolished reporter gene expression. Our results suggest that although no sequence similarity was found between mouse and Xenopus cerberus cis-regulatory regions, the signaling cascades regulating cerberus expression, during gastrulation, is conserved.
???displayArticle.pubmedLink??? 21509535
???displayArticle.link??? Dev Genes Evol
Species referenced: Xenopus
Genes referenced: cer1 dand5 nodal nodal1
References [+] :
Agius, Endodermal Nodal-related signals and mesoderm induction in Xenopus. 2000, Pubmed , Xenbase
Ahmed, Early endodermal expression of the Xenopus Endodermin gene is driven by regulatory sequences containing essential Sox protein-binding elements. 2004, Pubmed , Xenbase
Barnes, Embryonic expression of Lim-1, the mouse homolog of Xenopus Xlim-1, suggests a role in lateral mesoderm differentiation and neurogenesis. 1994, Pubmed , Xenbase
Bauer, The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus. 1994, Pubmed , Xenbase
Bell, Cell fate specification and competence by Coco, a maternal BMP, TGFbeta and Wnt inhibitor. 2003, Pubmed , Xenbase
Belo, Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula. 1997, Pubmed , Xenbase
Belo, Cerberus-like is a secreted BMP and nodal antagonist not essential for mouse development. 2000, Pubmed , Xenbase
Belo, The prechordal midline of the chondrocranium is defective in Goosecoid-1 mouse mutants. 1998, Pubmed
Belo, Generating asymmetries in the early vertebrate embryo: the role of the Cerberus-like family. 2009, Pubmed , Xenbase
Biben, Murine cerberus homologue mCer-1: a candidate anterior patterning molecule. 1998, Pubmed , Xenbase
Blum, Gastrulation in the mouse: the role of the homeobox gene goosecoid. 1992, Pubmed , Xenbase
Borges, Goosecoid and cerberus-like do not interact during mouse embryogenesis. 2002, Pubmed
Bouwmeester, Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. 1996, Pubmed , Xenbase
Brannon, A beta-catenin/XTcf-3 complex binds to the siamois promoter to regulate dorsal axis specification in Xenopus. 1997, Pubmed , Xenbase
Brennan, Nodal signalling in the epiblast patterns the early mouse embryo. 2001, Pubmed
Brickman, Hex is a transcriptional repressor that contributes to anterior identity and suppresses Spemann organiser function. 2000, Pubmed , Xenbase
Chen, Smad4 and FAST-1 in the assembly of activin-responsive factor. 1997, Pubmed , Xenbase
Cho, Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid. 1991, Pubmed , Xenbase
Clements, Redundant early and overlapping larval roles of Xsox17 subgroup genes in Xenopus endoderm development. 2003, Pubmed , Xenbase
De Roberts, Goosecoid and the organizer. 1992, Pubmed , Xenbase
Dufort, The transcription factor HNF3beta is required in visceral endoderm for normal primitive streak morphogenesis. 1998, Pubmed
Glinka, Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. 1998, Pubmed , Xenbase
Hanes, DNA specificity of the bicoid activator protein is determined by homeodomain recognition helix residue 9. 1989, Pubmed
Hashimoto, The Cerberus/Dan-family protein Charon is a negative regulator of Nodal signaling during left-right patterning in zebrafish. 2004, Pubmed , Xenbase
Hen, An enhancer element is located 340 base pairs upstream from the adenovirus-2 E1A capsite. 1983, Pubmed
Hudson, Xsox17alpha and -beta mediate endoderm formation in Xenopus. 1997, Pubmed , Xenbase
Ishimura, Involvement of BMP-4/msx-1 and FGF pathways in neural induction in the Xenopus embryo. 2000, Pubmed , Xenbase
Jones, An anterior signalling centre in Xenopus revealed by the homeobox gene XHex. 1999, Pubmed , Xenbase
Katoh, CER1 is a common target of WNT and NODAL signaling pathways in human embryonic stem cells. 2006, Pubmed
Kimura, Complementary functions of Otx2 and Cripto in initial patterning of mouse epiblast. 2001, Pubmed
Laurent, The Xenopus homeobox gene twin mediates Wnt induction of goosecoid in establishment of Spemann's organizer. 1997, Pubmed , Xenbase
Lerchner, Region-specific activation of the Xenopus brachyury promoter involves active repression in ectoderm and endoderm: a study using transgenic frog embryos. 2000, Pubmed , Xenbase
Liguori, Cripto-independent Nodal signaling promotes positioning of the A-P axis in the early mouse embryo. 2008, Pubmed
Maeda, Xmsx-1 modifies mesodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo. 1997, Pubmed , Xenbase
Marques, The activity of the Nodal antagonist Cerl-2 in the mouse node is required for correct L/R body axis. 2004, Pubmed
McKendry, LEF-1/TCF proteins mediate wnt-inducible transcription from the Xenopus nodal-related 3 promoter. 1997, Pubmed , Xenbase
Mesnard, The anterior-posterior axis emerges respecting the morphology of the mouse embryo that changes and aligns with the uterus before gastrulation. 2004, Pubmed
Mizuseki, Xenopus Zic-related-1 and Sox-2, two factors induced by chordin, have distinct activities in the initiation of neural induction. 1998, Pubmed , Xenbase
Mochizuki, Xlim-1 and LIM domain binding protein 1 cooperate with various transcription factors in the regulation of the goosecoid promoter. 2000, Pubmed , Xenbase
Moody, Fates of the blastomeres of the 32-cell-stage Xenopus embryo. 1987, Pubmed , Xenbase
Moody, Fates of the blastomeres of the 16-cell stage Xenopus embryo. 1987, Pubmed , Xenbase
Newman, The XHex homeobox gene is expressed during development of the vascular endothelium: overexpression leads to an increase in vascular endothelial cell number. 1997, Pubmed , Xenbase
Osada, Activin/nodal responsiveness and asymmetric expression of a Xenopus nodal-related gene converge on a FAST-regulated module in intron 1. 2000, Pubmed , Xenbase
Osada, Xenopus nodal-related signaling is essential for mesendodermal patterning during early embryogenesis. 1999, Pubmed , Xenbase
Pannese, The Xenopus homologue of Otx2 is a maternal homeobox gene that demarcates and specifies anterior body regions. 1995, Pubmed , Xenbase
Penzel, Characterization and early embryonic expression of a neural specific transcription factor xSOX3 in Xenopus laevis. 1997, Pubmed , Xenbase
Perea-Gomez, Role of the anterior visceral endoderm in restricting posterior signals in the mouse embryo. 2001, Pubmed
Perea-Gómez, HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo. 1999, Pubmed
Piccolo, The head inducer Cerberus is a multifunctional antagonist of Nodal, BMP and Wnt signals. 1999, Pubmed , Xenbase
Rebbert, Transcriptional regulation of the Xlim-1 gene by activin is mediated by an element in intron I. 1997, Pubmed , Xenbase
Rivera-Pérez, Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development. 1995, Pubmed , Xenbase
Rodríguez Esteban, The novel Cer-like protein Caronte mediates the establishment of embryonic left-right asymmetry. 1999, Pubmed , Xenbase
Ruiz i Altaba, Sequential expression of HNF-3 beta and HNF-3 alpha by embryonic organizing centers: the dorsal lip/node, notochord and floor plate. 1993, Pubmed , Xenbase
Saltzman, Promoter specificity and modulation of RNA polymerase II transcription. 1989, Pubmed
Sasai, Roles of Sox factors in neural determination: conserved signaling in evolution? 2001, Pubmed , Xenbase
Sekido, Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression. 1997, Pubmed
Shawlot, Expression of the mouse cerberus-related gene, Cerr1, suggests a role in anterior neural induction and somitogenesis. 1998, Pubmed , Xenbase
Silva, Endogenous Cerberus activity is required for anterior head specification in Xenopus. 2003, Pubmed , Xenbase
Simeone, Nested expression domains of four homeobox genes in developing rostral brain. 1992, Pubmed
Simeone, A vertebrate gene related to orthodenticle contains a homeodomain of the bicoid class and demarcates anterior neuroectoderm in the gastrulating mouse embryo. 1993, Pubmed
Steinbeisser, Xenopus goosecoid: a gene expressed in the prechordal plate that has dorsalizing activity. 1993, Pubmed , Xenbase
Taira, The LIM domain-containing homeo box gene Xlim-1 is expressed specifically in the organizer region of Xenopus gastrula embryos. 1992, Pubmed , Xenbase
Tavares, Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo. 2007, Pubmed
Thomas, Anterior primitive endoderm may be responsible for patterning the anterior neural plate in the mouse embryo. 1996, Pubmed
Thomas, Hex: a homeobox gene revealing peri-implantation asymmetry in the mouse embryo and an early transient marker of endothelial cell precursors. 1998, Pubmed
Trindade, DNA-binding specificity and embryological function of Xom (Xvent-2). 1999, Pubmed , Xenbase
van Grunsven, XSIP1, a Xenopus zinc finger/homeodomain encoding gene highly expressed during early neural development. 2000, Pubmed , Xenbase
Verschueren, SIP1, a novel zinc finger/homeodomain repressor, interacts with Smad proteins and binds to 5'-CACCT sequences in candidate target genes. 1999, Pubmed , Xenbase
Waldrip, Smad2 signaling in extraembryonic tissues determines anterior-posterior polarity of the early mouse embryo. 1998, Pubmed
Wilson, Cooperative dimerization of paired class homeo domains on DNA. 1993, Pubmed
Yamada, Targeted mutation of the murine goosecoid gene results in craniofacial defects and neonatal death. 1995, Pubmed
Yamamoto, Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling. 2001, Pubmed , Xenbase
Yamamoto, Molecular link in the sequential induction of the Spemann organizer: direct activation of the cerberus gene by Xlim-1, Xotx2, Mix.1, and Siamois, immediately downstream from Nodal and Wnt signaling. 2003, Pubmed , Xenbase
Yang, Roles of organizer factors and BMP antagonism in mammalian forebrain establishment. 2006, Pubmed
Yokouchi, Antagonistic signaling by Caronte, a novel Cerberus-related gene, establishes left-right asymmetric gene expression. 1999, Pubmed , Xenbase
Zhu, Cerberus regulates left-right asymmetry of the embryonic head and heart. 1999, Pubmed , Xenbase
Zorn, Anterior endomesoderm specification in Xenopus by Wnt/beta-catenin and TGF-beta signalling pathways. 1999, Pubmed , Xenbase