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Mol Cell Biol
1995 Dec 01;1512:6923-31. doi: 10.1128/MCB.15.12.6923.
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Specificity for the hairy/enhancer of split basic helix-loop-helix (bHLH) proteins maps outside the bHLH domain and suggests two separable modes of transcriptional repression.
Dawson SR
,
Turner DL
,
Weintraub H
,
Parkhurst SM
.
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The Hairy/Enhancer of split/Deadpan family of basic helix-loop-helix (bHLH) proteins function as transcriptional repressors. We have examined the mechanisms of repression used by the Hairy and E(SPL) proteins by assaying the antagonism between wild-type or altered Hairy/E(SPL) and Scute bHLH proteins during sex determination in Drosophila melanogaster. Domain swapping and mutagenesis of the Hairy and E(SPL) proteins show that three evolutionarily conserved domains are required for their function: the bHLH, Orange, and WRPW domains. However, the suppression of Scute activity by Hairy does not require the WRPW domain. We show that the Orange domain is an important functional domain that confers specificity among members of the Hairy/E(SPL) family. In addition, we show that a Xenopus Hairy homology conserves not only Hairy's structure but also its biological activity in our assays. We propose that transcriptional repression by the Hairy/E(SPL) family of bHLH proteins involves two separable mechanisms: repression of specific transcriptional activators, such as Scute, through the bHLH and Orange domains and repression of other activators via interaction of the C-terminal WRPW motif with corepressors, such as the Groucho protein.
Benezra,
The protein Id: a negative regulator of helix-loop-helix DNA binding proteins.
1990, Pubmed
Benezra,
The protein Id: a negative regulator of helix-loop-helix DNA binding proteins.
1990,
Pubmed
Bier,
deadpan, an essential pan-neural gene in Drosophila, encodes a helix-loop-helix protein similar to the hairy gene product.
1992,
Pubmed
Blackwell,
Binding of myc proteins to canonical and noncanonical DNA sequences.
1993,
Pubmed
Bopp,
Developmental distribution of female-specific Sex-lethal proteins in Drosophila melanogaster.
1991,
Pubmed
Botas,
Gene-dose titration analysis in the search of trans-regulatory genes in Drosophila.
1982,
Pubmed
Cabrera,
Transcriptional activation by heterodimers of the achaete-scute and daughterless gene products of Drosophila.
1991,
Pubmed
Cabrera,
Regulation of scute function by extramacrochaete in vitro and in vivo.
1994,
Pubmed
Cline,
Evidence that sisterless-a and sisterless-b are two of several discrete "numerator elements" of the X/A sex determination signal in Drosophila that switch Sxl between two alternative stable expression states.
1988,
Pubmed
Delidakis,
The Enhancer of split [E(spl)] locus of Drosophila encodes seven independent helix-loop-helix proteins.
1992,
Pubmed
Ellis,
extramacrochaetae, a negative regulator of sensory organ development in Drosophila, defines a new class of helix-loop-helix proteins.
1990,
Pubmed
Estes,
Multiple response elements in the Sex-lethal early promoter ensure its female-specific expression pattern.
1995,
Pubmed
Feder,
A rat gene with sequence homology to the Drosophila gene hairy is rapidly induced by growth factors known to influence neuronal differentiation.
1993,
Pubmed
Feder,
Genomic cloning and chromosomal localization of HRY, the human homolog to the Drosophila segmentation gene, hairy.
1994,
Pubmed
Ferré-D'Amaré,
Recognition by Max of its cognate DNA through a dimeric b/HLH/Z domain.
1993,
Pubmed
Fitzpatrick,
Homeodomain-independent activity of the fushi tarazu polypeptide in Drosophila embryos.
1992,
Pubmed
Garrell,
The Drosophila extramacrochaetae locus, an antagonist of proneural genes that, like these genes, encodes a helix-loop-helix protein.
1990,
Pubmed
Gray,
Short-range repression permits multiple enhancers to function autonomously within a complex promoter.
1994,
Pubmed
Herschbach,
Transcriptional repression in eukaryotes.
1993,
Pubmed
Higuchi,
A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.
1988,
Pubmed
Hiromi,
Regulation and function of the Drosophila segmentation gene fushi tarazu.
1987,
Pubmed
Ishibashi,
Molecular characterization of HES-2, a mammalian helix-loop-helix factor structurally related to Drosophila hairy and Enhancer of split.
1993,
Pubmed
Kagoshima,
The Runt domain identifies a new family of heteromeric transcriptional regulators.
1993,
Pubmed
Klämbt,
Closely related transcripts encoded by the neurogenic gene complex enhancer of split of Drosophila melanogaster.
1989,
Pubmed
Knust,
Seven genes of the Enhancer of split complex of Drosophila melanogaster encode helix-loop-helix proteins.
1992,
Pubmed
Levine,
Transcriptional repression of eukaryotic promoters.
1989,
Pubmed
Martínez,
Regulation of the proneural gene achaete by helix-loop-helix proteins.
1993,
Pubmed
Murre,
A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
1989,
Pubmed
Murre,
Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.
1989,
Pubmed
Nakanishi,
Accumulation of collagen III at the cleft points of developing mouse submandibular epithelium.
1988,
Pubmed
Ohsako,
Hairy function as a DNA-binding helix-loop-helix repressor of Drosophila sensory organ formation.
1994,
Pubmed
Parkhurst,
Sex determination and dosage compensation: lessons from flies and worms.
1994,
Pubmed
Parkhurst,
achaete-scute feminizing activities and Drosophila sex determination.
1993,
Pubmed
Parkhurst,
X:A ratio, the primary sex-determining signal in Drosophila, is transduced by helix-loop-helix proteins.
1990,
Pubmed
Parkhurst,
Mis-regulating segmentation gene expression in Drosophila.
1991,
Pubmed
Paroush,
Groucho is required for Drosophila neurogenesis, segmentation, and sex determination and interacts directly with hairy-related bHLH proteins.
1994,
Pubmed
Rushlow,
The Drosophila hairy protein acts in both segmentation and bristle patterning and shows homology to N-myc.
1989,
Pubmed
Sasai,
Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split.
1992,
Pubmed
Stifani,
Human homologs of a Drosophila Enhancer of split gene product define a novel family of nuclear proteins.
1992,
Pubmed
Tautz,
A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback.
1989,
Pubmed
Tietze,
Enhancer of splitD, a dominant mutation of Drosophila, and its use in the study of functional domains of a helix-loop-helix protein.
1992,
Pubmed
Torres,
The scute (T4) gene acts as a numerator element of the X:a signal that determines the state of activity of sex-lethal in Drosophila.
1989,
Pubmed
Van Doren,
The Drosophila extramacrochaetae protein antagonizes sequence-specific DNA binding by daughterless/achaete-scute protein complexes.
1991,
Pubmed
Van Doren,
Negative regulation of proneural gene activity: hairy is a direct transcriptional repressor of achaete.
1994,
Pubmed
Wainwright,
Point mutations in the Drosophila hairy gene demonstrate in vivo requirements for basic, helix-loop-helix, and WRPW domains.
1992,
Pubmed
Weintraub,
The myoD gene family: nodal point during specification of the muscle cell lineage.
1991,
Pubmed
Younger-Shepherd,
deadpan, an essential pan-neural gene encoding an HLH protein, acts as a denominator in Drosophila sex determination.
1992,
Pubmed
