Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-13558
Science 1999 Feb 05;2835403:851-4.
Show Gene links Show Anatomy links

Requirement of Cdk2-cyclin E activity for repeated centrosome reproduction in Xenopus egg extracts.

Hinchcliffe EH , Li C , Thompson EA , Maller JL , Sluder G .


???displayArticle.abstract???
The abnormally high number of centrosomes found in many human tumor cells can lead directly to aneuploidy and genomic instability through the formation of multipolar mitotic spindles. To facilitate investigation of the mechanisms that control centrosome reproduction, a frog egg extract arrested in S phase of the cell cycle that supported repeated assembly of daughter centrosomes was developed. Multiple rounds of centrosome reproduction were blocked by selective inactivation of cyclin-dependent kinase 2-cyclin E (Cdk2-E) and were restored by addition of purified Cdk2-E. Confocal immunomicroscopy revealed that cyclin E was localized at the centrosome. These results demonstrate that Cdk2-E activity is required for centrosome duplication during S phase and suggest a mechanism that could coordinate centrosome reproduction with cycles of DNA synthesis and mitosis.

???displayArticle.pubmedLink??? 9933170
???displayArticle.link??? Science


Species referenced: Xenopus
Genes referenced: cdk2

References :
Pennisi, Trigger for centrosome replication found. 1999, Pubmed, Xenbase