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In Vitro Cell Dev Biol Anim
2014 Apr 01;504:340-9. doi: 10.1007/s11626-013-9710-5.
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Knockdown of Pex11β reveals its pivotal role in regulating peroxisomal genes, numbers, and ROS levels in Xenopus laevis A6 cells.
Fox MA
,
Nieuwesteeg MA
,
Willson JA
,
Cepeda M
,
Damjanovski S
.
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Peroxisomes are organelles that are ubiquitously found in all eukaryotic cells. Enzymes within their lumen are responsible for a variety of processes including the metabolism of fatty acids and eradication (neutralization) of free radicals. Peroxisomes are dynamic organelles, able to alter their numbers in response to a variety of different metabolic and cell-specific cues. Changes in peroxisome numbers can occur through division of preexisting peroxisomes or through de novo biogenesis from the ER. Proteins such as the Pex11 family of peroxins have been implicated as regulatory factors involved in peroxisome division. Division of peroxisomes involves elongation and membrane constriction followed by fission, which requires Pex11β. The regulation of peroxisome numbers in different cell types and tissues is variable and poorly understood. Here, we examine how knockdown of Pex11β affects peroxisomal genes, proteins, and peroxisome numbers in A6 kidney epithelial cells derived from Xenopus laevis. Pex11β morpholino use subsequently decreased mRNA levels of Pex1, PMP70, and PPARγ. Moreover, the Pex11β morpholino decreased PMP70 protein levels and PMP70-positive structures. Furthermore, the marker GFP-SKL revealed fewer peroxisome-like structures. These decreases resulted in increased levels of H2O2 and cellular and mitochondrial reactive oxygen species as measured by Amplex Red, DCFDA, and MitoTracker assays, respectively.
Ahlemeyer,
Deletion of a single allele of the Pex11β gene is sufficient to cause oxidative stress, delayed differentiation and neuronal death in mouse brain.
2012, Pubmed
Ahlemeyer,
Deletion of a single allele of the Pex11β gene is sufficient to cause oxidative stress, delayed differentiation and neuronal death in mouse brain.
2012,
Pubmed
Bonekamp,
Reactive oxygen species and peroxisomes: struggling for balance.
2009,
Pubmed
Bonekamp,
Self-interaction of human Pex11pβ during peroxisomal growth and division.
2013,
Pubmed
Campbell,
A role for peroxisome proliferator-activated receptor alpha (PPARalpha ) in the control of cardiac malonyl-CoA levels: reduced fatty acid oxidation rates and increased glucose oxidation rates in the hearts of mice lacking PPARalpha are associated with higher concentrations of malonyl-CoA and reduced expression of malonyl-CoA decarboxylase.
2002,
Pubmed
Ciolek,
The effect of clofibrate on amphibian hepatic peroxisomes.
1991,
Pubmed
,
Xenbase
Delille,
Pex11pβ-mediated maturation of peroxisomes.
2011,
Pubmed
Diano,
Peroxisome proliferation-associated control of reactive oxygen species sets melanocortin tone and feeding in diet-induced obesity.
2011,
Pubmed
Fox,
PEX11β induces peroxisomal gene expression and alters peroxisome number during early Xenopus laevis development.
2011,
Pubmed
,
Xenbase
Fransen,
Role of peroxisomes in ROS/RNS-metabolism: implications for human disease.
2012,
Pubmed
Fujiki,
New insights into dynamic and functional assembly of the AAA peroxins, Pex1p and Pex6p, and their membrane receptor Pex26p in shuttling of PTS1-receptor Pex5p during peroxisome biogenesis.
2012,
Pubmed
Goto,
Activation of peroxisome proliferator-activated receptor-alpha stimulates both differentiation and fatty acid oxidation in adipocytes.
2011,
Pubmed
Imanaka,
The 70-kDa peroxisomal membrane protein (PMP70), an ATP-binding cassette transporter.
2000,
Pubmed
Islinger,
Be different--the diversity of peroxisomes in the animal kingdom.
2010,
Pubmed
Kobayashi,
Fis1, DLP1, and Pex11p coordinately regulate peroxisome morphogenesis.
2007,
Pubmed
Koch,
Dynamin-like protein 1 is involved in peroxisomal fission.
2003,
Pubmed
Koch,
PEX11 proteins attract Mff and human Fis1 to coordinate peroxisomal fission.
2012,
Pubmed
Koch,
Membrane elongation factors in organelle maintenance: the case of peroxisome proliferation.
2011,
Pubmed
Krause,
Identification of novel mutations in PEX2, PEX6, PEX10, PEX12, and PEX13 in Zellweger spectrum patients.
2006,
Pubmed
Legakis,
Peroxisome senescence in human fibroblasts.
2002,
Pubmed
Li,
The dynamin-like GTPase DLP1 is essential for peroxisome division and is recruited to peroxisomes in part by PEX11.
2003,
Pubmed
Lopez-Huertas,
Stress induces peroxisome biogenesis genes.
2000,
Pubmed
Ma,
Peroxisome matrix and membrane protein biogenesis.
2009,
Pubmed
Ma,
Peroxisome assembly: matrix and membrane protein biogenesis.
2011,
Pubmed
Opaliński,
Membrane curvature during peroxisome fission requires Pex11.
2011,
Pubmed
Orth,
The PEROXIN11 protein family controls peroxisome proliferation in Arabidopsis.
2007,
Pubmed
Reuber,
Mutations in PEX1 are the most common cause of peroxisome biogenesis disorders.
1997,
Pubmed
Rucktäschel,
De novo synthesis of peroxisomes upon mitochondrial targeting of Pex3p.
2010,
Pubmed
Schrader,
Peroxisomes and oxidative stress.
2006,
Pubmed
Shiozawa,
Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain.
2004,
Pubmed
Thoms,
Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation.
2005,
Pubmed
Titorenko,
Peroxisome metabolism and cellular aging.
2011,
Pubmed
Waterham,
Genetics and molecular basis of human peroxisome biogenesis disorders.
2012,
Pubmed
