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BMC Res Notes
2014 Nov 26;7:850. doi: 10.1186/1756-0500-7-850.
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First-generation linkage map for the European tree frog (Hyla arborea) with utility in congeneric species.
Dufresnes C
,
Brelsford A
,
Perrin N
.
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Western Palearctic tree frogs (Hyla arborea group) represent a strong potential for evolutionary and conservation genetic research, so far underexploited due to limited molecular resources. New microsatellite markers have recently been developed for Hyla arborea, with high cross-species utility across the entire circum-Mediterranean radiation. Here we conduct sibship analyses to map available markers for use in future population genetic applications. We characterized eight linkage groups, including one sex-linked, all showing drastically reduced recombination in males compared to females, as previously documented in this species. Mapping of the new 15 markers to the ~200 My diverged Xenopus tropicalis genome suggests a generally conserved synteny with only one confirmed major chromosome rearrangement. The new microsatellites are representative of several chromosomes of H. arborea that are likely to be conserved across closely-related species. Our linkage map provides an important resource for genetic research in European Hylids, notably for studies of speciation, genome evolution and conservation.
Figure 1.
Male- and female-specific linkage maps for
Hyla arborea. Maps are based on 43 microsatellites and one SNP locus (Fryl), with orders according to the highest log likelihood. For LG7, marker Ha-T64 was not polymorphic in females. Genetic distances are indicated in centiMorgan (cM). Colored frames show the location of homologous loci within the Xenopus tropicalis genome (assembly 7.1, http://xenbase.org), as reported in [8]. For clarity, simplified marker names were used (see Additional file 1 for full identifiers).
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