XB-ART-2929
Science
2004 Oct 01;3065693:117-20. doi: 10.1126/science.1100946.
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Ubistatins inhibit proteasome-dependent degradation by binding the ubiquitin chain.
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To identify previously unknown small molecules that inhibit cell cycle machinery, we performed a chemical genetic screen in Xenopus extracts. One class of inhibitors, termed ubistatins, blocked cell cycle progression by inhibiting cyclin B proteolysis and inhibited degradation of ubiquitinated Sic1 by purified proteasomes. Ubistatins blocked the binding of ubiquitinated substrates to the proteasome by targeting the ubiquitin-ubiquitin interface of Lys(48)-linked chains. The same interface is recognized by ubiquitin-chain receptors of the proteasome, indicating that ubistatins act by disrupting a critical protein-protein interaction in the ubiquitin-proteasome system.
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Species referenced: Xenopus laevis
Genes referenced: ccnb1.2
References :