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We have raised a monoclonal antibody, 4G6, against gut manually isolated from stage 42Xenopus laevis embryos. It is specific for endoderm and recognises an epitope that is first expressed at stage 19 and which persists throughout subsequent development. The antibody maintains gut specificity through metamorphosis and into adulthood. The epitope is conserved in the mouse, where it is also found in the gut. Isolated vegetal poles fromXenopus blastula stage embryos express the epitope autonomously after culturing to the appropriate stage. This shows that certain aspects of endoderm differentiation do not require germ layer interactions. Animal cap cells from stage 9 blastulae cultured in the presence of the mesodermal growth factors FGF, XTC-MIF and PIF form both endodermal and mesodermal tissues, assessed by the binding of tissue-specific monoclonal antibodies. Endoderm is typically found in those caps which form intermediate and ventral forms of mesoderm, that is muscle and lateral plate.
Albano,
A mesoderm-inducing factor produced by WEHI-3 murine myelomonocytic leukemia cells is activin A.
1990, Pubmed,
Xenbase
Albano,
A mesoderm-inducing factor produced by WEHI-3 murine myelomonocytic leukemia cells is activin A.
1990,
Pubmed
,
Xenbase
Amaya,
Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Asashima,
The vegetalizing factor from chicken embryos: its EDF (activin A)-like activity.
1991,
Pubmed
Dale,
Fate map for the 32-cell stage of Xenopus laevis.
1987,
Pubmed
,
Xenbase
Gillespie,
Analysis of competence: receptors for fibroblast growth factor in early Xenopus embryos.
1989,
Pubmed
,
Xenbase
Green,
The biological effects of XTC-MIF: quantitative comparison with Xenopus bFGF.
1990,
Pubmed
,
Xenbase
Grunz,
Induction of mesodermal tissues by acidic and basic heparin binding growth factors.
1988,
Pubmed
,
Xenbase
Grunz,
Change in the differentiation pattern ofXenopus laevis ectoderm by variation of the incubation time and concentration of vegetalizing factor.
1983,
Pubmed
,
Xenbase
Grunz,
[Relationship between competence and protein synthesis in amphibian ectoderm].
1970,
Pubmed
Hausen,
The use of polyacrylamide as an embedding medium for immunohistochemical studies of embryonic tissues.
1981,
Pubmed
Jones,
Spatial aspects of neural induction in Xenopus laevis.
1989,
Pubmed
,
Xenbase
Kimelman,
Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
1987,
Pubmed
,
Xenbase
Knöchel,
Mesoderm-inducing factors. Their possible relationship to heparin-binding growth factors and transforming growth factor-beta.
1987,
Pubmed
Kocher-Becker,
Induction of mesodermal and endodermal structures and primordial germ cells in Triturus ectoderm by a vegetalizing factor from chick embryos.
1971,
Pubmed
Köhler,
Continuous cultures of fused cells secreting antibody of predefined specificity.
1975,
Pubmed
Minuth,
The formation of mesodermal derivatives after induction with vegetalizing factor depends on secondary cell interactions.
1980,
Pubmed
Moody,
Fates of the blastomeres of the 32-cell-stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Nieuwkoop,
The formation of the mesoderm in urodelean amphibians : I. Induction by the endoderm.
1969,
Pubmed
Nieuwkoop,
Origin and establishment of embryonic polar axes in amphibian development.
1977,
Pubmed
Rosa,
Mesoderm induction in amphibians: the role of TGF-beta 2-like factors.
1988,
Pubmed
,
Xenbase
Slack,
Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
,
Pubmed
,
Xenbase
Slack,
An interaction between dorsal and ventral regions of the marginal zone in early amphibian embryos.
1980,
Pubmed
,
Xenbase
Smith,
Mesoderm induction and mesoderm-inducing factors in early amphibian development.
1989,
Pubmed
Smith,
A mesoderm-inducing factor is produced by Xenopus cell line.
1987,
Pubmed
,
Xenbase
Smith,
Purification, partial characterization and biological effects of the XTC mesoderm-inducing factor.
1988,
Pubmed
,
Xenbase
Smith,
Identification of a potent Xenopus mesoderm-inducing factor as a homologue of activin A.
1990,
Pubmed
,
Xenbase
Thomsen,
Activins are expressed early in Xenopus embryogenesis and can induce axial mesoderm and anterior structures.
1990,
Pubmed
,
Xenbase
Tiedemann,
The vegetalizing factor. A member of the evolutionarily highly conserved activin family.
1992,
Pubmed
,
Xenbase
Wylie,
Vegetal pole cells and commitment to form endoderm in Xenopus laevis.
1987,
Pubmed
,
Xenbase
