Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
We have identified a double-stranded (ds)RNA-binding domain in each of two proteins: the product of the Drosophila gene staufen, which is required for the localization of maternal mRNAs, and a protein of unknown function, Xlrbpa, from Xenopus. The amino acid sequences of the binding domains are similar to each other and to additional domains in each protein. Database searches identified similar domains in several other proteins known or thought to bind dsRNA, including human dsRNA-activated inhibitor (DAI), human trans-activating region (TAR)-binding protein, and Escherichia coli RNase III. By analyzing in detail one domain in staufen and one in Xlrbpa, we delimited the minimal region that binds dsRNA. On the basis of the binding studies and computer analysis, we have derived a consensus sequence that defines a 65- to 68-amino acid dsRNA-binding domain.
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986, Pubmed
Adam,
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
1986,
Pubmed
Ahn,
Identification of rpo30, a vaccinia virus RNA polymerase gene with structural similarity to a eucaryotic transcription elongation factor.
1990,
Pubmed
Akusjärvi,
Structure of genes for virus-associated RNAI and RNAII of adenovirus type 2.
1980,
Pubmed
Bandziulis,
RNA-binding proteins as developmental regulators.
1989,
Pubmed
Broyles,
Vaccinia virus gene encoding a 30-kilodalton subunit of the viral DNA-dependent RNA polymerase.
1990,
Pubmed
Chang,
The E3L gene of vaccinia virus encodes an inhibitor of the interferon-induced, double-stranded RNA-dependent protein kinase.
1992,
Pubmed
Collins,
The significance of protein sequence similarities.
1988,
Pubmed
Devereux,
A comprehensive set of sequence analysis programs for the VAX.
1984,
Pubmed
Ephrussi,
Oskar organizes the germ plasm and directs localization of the posterior determinant nanos.
1991,
Pubmed
Feng,
Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.
1992,
Pubmed
Gallegos,
Characterization of rotavirus replication intermediates: a model for the assembly of single-shelled particles.
1989,
Pubmed
Gatignol,
Characterization of a human TAR RNA-binding protein that activates the HIV-1 LTR.
1991,
Pubmed
Gribskov,
Profile analysis: detection of distantly related proteins.
1987,
Pubmed
Hamm,
Functional analysis of mutant Xenopus U2 snRNAs.
1989,
Pubmed
,
Xenbase
Hovanessian,
The double stranded RNA-activated protein kinase induced by interferon: dsRNA-PK.
1989,
Pubmed
Icely,
TIK, a novel serine/threonine kinase, is recognized by antibodies directed against phosphotyrosine.
1991,
Pubmed
Iino,
S. pombe pac1+, whose overexpression inhibits sexual development, encodes a ribonuclease III-like RNase.
1991,
Pubmed
Kenan,
RNA recognition: towards identifying determinants of specificity.
1991,
Pubmed
Kim-Ha,
oskar mRNA is localized to the posterior pole of the Drosophila oocyte.
1991,
Pubmed
Levin,
Characterization of double-stranded-RNA-activated kinase that phosphorylates alpha subunit of eukaryotic initiation factor 2 (eIF-2 alpha) in reticulocyte lysates.
1980,
Pubmed
Macdonald,
cis-acting sequences responsible for anterior localization of bicoid mRNA in Drosophila embryos.
1988,
Pubmed
MacDonald,
bicoid mRNA localization signal: phylogenetic conservation of function and RNA secondary structure.
1990,
Pubmed
March,
The DNA sequence of the gene (rnc) encoding ribonuclease III of Escherichia coli.
1985,
Pubmed
McCormack,
Mechanism of interferon action: identification of a RNA binding domain within the N-terminal region of the human RNA-dependent P1/eIF-2 alpha protein kinase.
1992,
Pubmed
Meurs,
Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon.
1990,
Pubmed
Pearson,
Improved tools for biological sequence comparison.
1988,
Pubmed
Qian,
Molecular analysis of the gene 6 from a porcine group C rotavirus that encodes the NS34 equivalent of group A rotaviruses.
1991,
Pubmed
Robertson,
Purification and properties of ribonuclease III from Escherichia coli.
1968,
Pubmed
Saito,
Processing of mRNA by ribonuclease III regulates expression of gene 1.2 of bacteriophage T7.
1981,
Pubmed
Smith,
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
1988,
Pubmed
St Johnston,
Staufen, a gene required to localize maternal RNAs in the Drosophila egg.
1991,
Pubmed
St Johnston,
Multiple steps in the localization of bicoid RNA to the anterior pole of the Drosophila oocyte.
1989,
Pubmed
Tafuri,
Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation.
1990,
Pubmed
,
Xenbase
Thomis,
Mechanism of interferon action: cDNA structure, expression, and regulation of the interferon-induced, RNA-dependent P1/eIF-2 alpha protein kinase from human cells.
1992,
Pubmed
Vinson,
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
1988,
Pubmed
Watson,
Characterization of a vaccinia virus-encoded double-stranded RNA-binding protein that may be involved in inhibition of the double-stranded RNA-dependent protein kinase.
1991,
Pubmed
