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Mol Biol Cell
2003 Mar 01;143:1125-37. doi: 10.1091/mbc.e02-08-0511.
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Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts.
Beckhelling C
,
Chang P
,
Chevalier S
,
Ford C
,
Houliston E
.
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We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising "annulate lamellae" (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates.
Alfa,
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
1990, Pubmed
Alfa,
Distinct nuclear and spindle pole body population of cyclin-cdc2 in fission yeast.
1990,
Pubmed
Argon,
GRP94, an ER chaperone with protein and peptide binding properties.
1999,
Pubmed
Ashcroft,
RNA-Mediated interference of a cdc25 homolog in Caenorhabditis elegans results in defects in the embryonic cortical membrane, meiosis, and mitosis.
1999,
Pubmed
Audit,
Cyclin B (p56cdc13) localization in the yeast Schizosaccharomyces pombe: an ultrastructural and immunocytochemical study.
1996,
Pubmed
Bagley,
The nuclear pore complex.
2000,
Pubmed
Bailly,
p34cdc2 is located in both nucleus and cytoplasm; part is centrosomally associated at G2/M and enters vesicles at anaphase.
1989,
Pubmed
Bailly,
Cytoplasmic accumulation of cyclin B1 in human cells: association with a detergent-resistant compartment and with the centrosome.
1992,
Pubmed
Bal,
Origin and fate of annulate lamellae in Arbacia punctulata eggs.
1968,
Pubmed
Beaudouin,
Nuclear envelope breakdown proceeds by microtubule-induced tearing of the lamina.
2002,
Pubmed
Beckhelling,
Timing of calcium and protein synthesis requirements for the first mitotic cell cycle in fertilised Xenopus eggs.
1999,
Pubmed
,
Xenbase
Beckhelling,
Maturation promoting factor activation in early amphibian embryos: temporal and spatial control.
1998,
Pubmed
Beckhelling,
Localised MPF regulation in eggs.
2000,
Pubmed
,
Xenbase
Borgne,
Intra-M phase-promoting factor phosphorylation of cyclin B at the prophase/metaphase transition.
1999,
Pubmed
,
Xenbase
Brunati,
GRP94 (endoplasmin) co-purifies with and is phosphorylated by Golgi apparatus casein kinase.
2000,
Pubmed
Charrasse,
The Xenopus XMAP215 and its human homologue TOG proteins interact with cyclin B1 to target p34cdc2 to microtubules during mitosis.
2000,
Pubmed
,
Xenbase
Clute,
Temporal and spatial control of cyclin B1 destruction in metaphase.
1999,
Pubmed
Cordes,
Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior.
1996,
Pubmed
Cyert,
Regulation of MPF activity in vitro.
1988,
Pubmed
,
Xenbase
Dabauvalle,
Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin.
1991,
Pubmed
,
Xenbase
De Souza,
Centrosomal and cytoplasmic Cdc2/cyclin B1 activation precedes nuclear mitotic events.
2000,
Pubmed
DETTLAFF,
THE R OLE OF THE GERMINAL VESICLE IN OOECYTE MATURATION IN ANURANS AS REVEALED BY THE REMOVAL AND TRANSPLANTATION OF NUCLEI.
1964,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Edgar,
Distinct molecular mechanism regulate cell cycle timing at successive stages of Drosophila embryogenesis.
1994,
Pubmed
Felix,
A post-ribosomal supernatant from activated Xenopus eggs that displays post-translationally regulated oscillation of its cdc2+ mitotic kinase activity.
1989,
Pubmed
,
Xenbase
Gerhart,
Cell cycle dynamics of an M-phase-specific cytoplasmic factor in Xenopus laevis oocytes and eggs.
1984,
Pubmed
,
Xenbase
Hagting,
Translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal.
1999,
Pubmed
Hang,
Association of the human SUMO-1 protease SENP2 with the nuclear pore.
2002,
Pubmed
,
Xenbase
Hara,
A cytoplasmic clock with the same period as the division cycle in Xenopus eggs.
1980,
Pubmed
,
Xenbase
Hartley,
In vivo regulation of the early embryonic cell cycle in Xenopus.
1996,
Pubmed
,
Xenbase
Heim,
Improved green fluorescence.
1995,
Pubmed
Imreh,
An integral membrane protein from the nuclear pore complex is also present in the annulate lamellae: implications for annulate lamella formation.
2000,
Pubmed
Iwao,
The egg nucleus regulates the behavior of sperm nuclei as well as cycling of MPF in physiologically polyspermic newt eggs.
1993,
Pubmed
,
Xenbase
Iwao,
Control of sperm nuclear behavior in physiologically polyspermic newt eggs: possible involvement of MPF.
1990,
Pubmed
,
Xenbase
Iwashita,
Essential role of germinal vesicle material in the meiotic cell cycle of Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Jackman,
Human cyclins B1 and B2 are localized to strikingly different structures: B1 to microtubules, B2 primarily to the Golgi apparatus.
1995,
Pubmed
Kallenbach,
'De novo' centrioles originate at sites associated with annulate lamellae in sea-urchin eggs.
1982,
Pubmed
Kessel,
Annulate lamellae: a last frontier in cellular organelles.
1992,
Pubmed
Kimelman,
The events of the midblastula transition in Xenopus are regulated by changes in the cell cycle.
1987,
Pubmed
,
Xenbase
Kobayashi,
On the synthesis and destruction of A- and B-type cyclins during oogenesis and meiotic maturation in Xenopus laevis.
1991,
Pubmed
,
Xenbase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Leiss,
Association of cyclin-bound p34cdc2 with subcellular structures in xenopus eggs.
1992,
Pubmed
,
Xenbase
Li,
Nuclear localization of cyclin B1 mediates its biological activity and is regulated by phosphorylation.
1997,
Pubmed
,
Xenbase
Lindsay,
Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts.
1995,
Pubmed
,
Xenbase
Liu,
The human Myt1 kinase preferentially phosphorylates Cdc2 on threonine 14 and localizes to the endoplasmic reticulum and Golgi complex.
1997,
Pubmed
,
Xenbase
Masui,
Cytoplasmic control of nuclear behavior during meiotic maturation of frog oocytes.
1971,
Pubmed
Meier,
Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation.
1995,
Pubmed
,
Xenbase
Moreno,
Substrates for p34cdc2: in vivo veritas?
1990,
Pubmed
Morgan,
Regulation of the APC and the exit from mitosis.
1999,
Pubmed
Morgan,
Cyclin-dependent kinases: engines, clocks, and microprocessors.
1997,
Pubmed
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
The role of cyclin synthesis and degradation in the control of maturation promoting factor activity.
1989,
Pubmed
,
Xenbase
Newport,
Regulation of the cell cycle during early Xenopus development.
1984,
Pubmed
,
Xenbase
Nigg,
Cyclin-dependent protein kinases: key regulators of the eukaryotic cell cycle.
1995,
Pubmed
Norbury,
Animal cell cycles and their control.
1992,
Pubmed
Novak,
Numerical analysis of a comprehensive model of M-phase control in Xenopus oocyte extracts and intact embryos.
1993,
Pubmed
,
Xenbase
Nurse,
Universal control mechanism regulating onset of M-phase.
1990,
Pubmed
O'Farrell,
Triggering the all-or-nothing switch into mitosis.
2001,
Pubmed
Ohi,
Regulating the onset of mitosis.
1999,
Pubmed
,
Xenbase
Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed
Panté,
Interactions and three-dimensional localization of a group of nuclear pore complex proteins.
1994,
Pubmed
,
Xenbase
Pérez-Mongiovi,
A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus.
1998,
Pubmed
,
Xenbase
Pérez-Mongiovi,
Nuclei and microtubule asters stimulate maturation/M phase promoting factor (MPF) activation in Xenopus eggs and egg cytoplasmic extracts.
2000,
Pubmed
,
Xenbase
Pines,
Four-dimensional control of the cell cycle.
1999,
Pubmed
Pitt,
P granules in the germ cells of Caenorhabditis elegans adults are associated with clusters of nuclear pores and contain RNA.
2000,
Pubmed
Pockwinse,
Cell cycle independent interaction of CDC2 with the centrosome, which is associated with the nuclear matrix-intermediate filament scaffold.
1997,
Pubmed
Raff,
Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos.
1990,
Pubmed
Rankin,
The surface contraction waves of Xenopus eggs reflect the metachronous cell-cycle state of the cytoplasm.
1997,
Pubmed
,
Xenbase
Reinsch,
Movement of nuclei along microtubules in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Rieder,
Intranuclear membranes and the formation of the first meiotic spindle in Xenos peckii (Acroschismus wheeleri) oocytes.
1983,
Pubmed
Roegiers,
Phases of cytoplasmic and cortical reorganizations of the ascidian zygote between fertilization and first division.
1999,
Pubmed
Sakamoto,
Changes in cyclin B during oocyte maturation and early embryonic cell cycle in the newt, Cynops pyrrhogaster: requirement of germinal vesicle for MPF activation.
1998,
Pubmed
Salina,
Cytoplasmic dynein as a facilitator of nuclear envelope breakdown.
2002,
Pubmed
Shinagawa,
Nuclear Involvement in Localization of the Initiation Site of Surface Contraction Waves in Xenopus Eggs: (surface contraction waves/Xenopus laevis/nucleus/colchicine/cleavage).
1989,
Pubmed
,
Xenbase
Shinagawa,
The interval of the cytoplasmic cycle observed in non-nucleate egg fragments is longer than that of the cleavage cycle in normal eggs of Xenopus laevis.
1983,
Pubmed
,
Xenbase
Shinagawa,
Relative Timing of Stiffening with Various Combinations of Nucleate and Enucleated Egg Fragments of Xenopus laevis: (egg stiffening/Xenopus laevis/cell cycle/timing system).
1992,
Pubmed
,
Xenbase
Solomon,
Cyclin activation of p34cdc2.
1990,
Pubmed
,
Xenbase
Takahashi,
Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins.
2000,
Pubmed
Takizawa,
Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C.
2000,
Pubmed
,
Xenbase
Terasaki,
Changes in organization of the endoplasmic reticulum during Xenopus oocyte maturation and activation.
2001,
Pubmed
,
Xenbase
Wakefield,
Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos.
2000,
Pubmed
Xu,
Mouse VDAC isoforms expressed in yeast: channel properties and their roles in mitochondrial outer membrane permeability.
1999,
Pubmed
Yang,
Control of cyclin B1 localization through regulated binding of the nuclear export factor CRM1.
1998,
Pubmed
,
Xenbase
Zernicka-Goetz,
An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
1996,
Pubmed
,
Xenbase
