Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The N-terminus of the K channel KAT1 controls its voltage-dependent gating by altering the membrane electric field.
Marten I
,
Hoshi T
.
???displayArticle.abstract???
Functional roles of different domains (pore region, S4 segment, N-terminus) of the KAT1 potassium channel in its voltage-dependent gating were electrophysiologically studied in Xenopus oocytes. The KAT1 properties did not depend on the extracellular K+ concentration or on residue H267, equivalent to one of the residues known to be important in C-type inactivation in Shaker channels, indicating that the hyperpolarization-induced KAT1 inward currents are related to the channel activation rather than to recovery from inactivation. Neutralization of a positively charged amino acid in the S4 domain (R176S) reduced the gating charge movement, suggesting that it acts as a voltage-sensing residue in KAT1. N-terminal deletions alone (e.g., delta20-34) did not affect the gating charge movement. However, the deletions paradoxically increased the voltage sensitivity of the R176S mutant channel, but not that of the wild-type channel. We propose a simple model in which the N-terminus determines the KAT1 voltage sensitivity by contributing to the electric field sensed by the voltage sensor.
Aldrich,
Potassium channels. New channel subunits are a turn-off.
1994, Pubmed,
Xenbase
Aldrich,
Potassium channels. New channel subunits are a turn-off.
1994,
Pubmed
,
Xenbase
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Auld,
A neutral amino acid change in segment IIS4 dramatically alters the gating properties of the voltage-dependent sodium channel.
1990,
Pubmed
,
Xenbase
Choi,
Tetraethylammonium blockade distinguishes two inactivation mechanisms in voltage-activated K+ channels.
1991,
Pubmed
Curran,
A molecular basis for cardiac arrhythmia: HERG mutations cause long QT syndrome.
1995,
Pubmed
Daram,
Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain.
1997,
Pubmed
Dreyer,
Plant K+ channel alpha-subunits assemble indiscriminately.
1997,
Pubmed
,
Xenbase
García,
Role of S4 segments and the leucine heptad motif in the activation of an L-type calcium channel.
1997,
Pubmed
Hoshi,
Regulation of voltage dependence of the KAT1 channel by intracellular factors.
1995,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Hoshi,
Two types of inactivation in Shaker K+ channels: effects of alterations in the carboxy-terminal region.
1991,
Pubmed
,
Xenbase
Jan,
Cloned potassium channels from eukaryotes and prokaryotes.
1997,
Pubmed
Larsson,
Transmembrane movement of the shaker K+ channel S4.
1996,
Pubmed
,
Xenbase
Levy,
Recovery from C-type inactivation is modulated by extracellular potassium.
1996,
Pubmed
Li,
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel.
1992,
Pubmed
López-Barneo,
Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels.
1993,
Pubmed
,
Xenbase
Mannuzzu,
Direct physical measure of conformational rearrangement underlying potassium channel gating.
1996,
Pubmed
,
Xenbase
Marten,
Voltage-dependent gating characteristics of the K+ channel KAT1 depend on the N and C termini.
1997,
Pubmed
McCormack,
A role for hydrophobic residues in the voltage-dependent gating of Shaker K+ channels.
1991,
Pubmed
,
Xenbase
Miller,
Conversion of a delayed rectifier K+ channel to a voltage-gated inward rectifier K+ channel by three amino acid substitutions.
1996,
Pubmed
Ogielska,
Cooperative subunit interactions in C-type inactivation of K channels.
1995,
Pubmed
,
Xenbase
Papazian,
Electrostatic interactions of S4 voltage sensor in Shaker K+ channel.
1995,
Pubmed
,
Xenbase
Pardo,
Extracellular K+ specifically modulates a rat brain K+ channel.
1992,
Pubmed
,
Xenbase
Pearson,
Protein kinase phosphorylation site sequences and consensus specificity motifs: tabulations.
1991,
Pubmed
Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase
Sanguinetti,
A mechanistic link between an inherited and an acquired cardiac arrhythmia: HERG encodes the IKr potassium channel.
1995,
Pubmed
,
Xenbase
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Schlief,
Modification of C-type inactivating Shaker potassium channels by chloramine-T.
1996,
Pubmed
,
Xenbase
Schönherr,
Molecular determinants for activation and inactivation of HERG, a human inward rectifier potassium channel.
1996,
Pubmed
,
Xenbase
Seoh,
Voltage-sensing residues in the S2 and S4 segments of the Shaker K+ channel.
1996,
Pubmed
,
Xenbase
Sigworth,
Voltage gating of ion channels.
1994,
Pubmed
Smith,
The inward rectification mechanism of the HERG cardiac potassium channel.
1996,
Pubmed
Spector,
Fast inactivation causes rectification of the IKr channel.
1996,
Pubmed
,
Xenbase
Terlau,
Amino terminal-dependent gating of the potassium channel rat eag is compensated by a mutation in the S4 segment.
1997,
Pubmed
,
Xenbase
Tiwari-Woodruff,
Electrostatic interactions between transmembrane segments mediate folding of Shaker K+ channel subunits.
1997,
Pubmed
,
Xenbase
Trudeau,
HERG, a human inward rectifier in the voltage-gated potassium channel family.
1995,
Pubmed
Véry,
Expression of a cloned plant K+ channel in Xenopus oocytes: analysis of macroscopic currents.
1995,
Pubmed
,
Xenbase
Warmke,
A family of potassium channel genes related to eag in Drosophila and mammals.
1994,
Pubmed
Zagotta,
Shaker potassium channel gating. II: Transitions in the activation pathway.
1994,
Pubmed
,
Xenbase
