Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Improved detection of electrical activity with a voltage probe based on a voltage-sensing phosphatase.
Tsutsui H
,
Jinno Y
,
Tomita A
,
Niino Y
,
Yamada Y
,
Mikoshiba K
,
Miyawaki A
,
Okamura Y
.
???displayArticle.abstract???
One of the most awaited techniques in modern physiology is the sensitive detection of spatiotemporal electrical activity in a complex network of excitable cells. The use of genetically encoded voltage probes has been expected to enable such analysis. However, in spite of recent progress, existing probes still suffer from low signal amplitude and/or kinetics too slow to detect fast electrical activity. Here, we have developed an improved voltage probe named Mermaid2, which is based on the voltage-sensor domain of the voltage-sensing phosphatase from Ciona intestinalis and Förster energy transfer between a pair of fluorescent proteins. In mammalian cells, Mermaid2 permits ratiometric readouts of fractional changes of more than 50% over a physiologically relevant voltage range with fast kinetics, and it was used to follow a train of action potentials at frequencies of up to 150 Hz. Mermaid2 was also able to detect single action potentials and subthreshold voltage responses in hippocampal neurons in vitro, in addition to cortical electrical activity evoked by sound stimuli in single trials in living mice.
Akemann,
Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins.
2010, Pubmed
Akemann,
Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins.
2010,
Pubmed
Akemann,
Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein.
2012,
Pubmed
,
Xenbase
Ataka,
A genetically targetable fluorescent probe of channel gating with rapid kinetics.
2002,
Pubmed
Baker,
Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells.
2007,
Pubmed
Baker,
Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics.
2012,
Pubmed
Barnett,
A fluorescent, genetically-encoded voltage probe capable of resolving action potentials.
2012,
Pubmed
Dimitrov,
Engineering and characterization of an enhanced fluorescent protein voltage sensor.
2007,
Pubmed
Drummond,
Reporting ethical matters in the Journal of Physiology: standards and advice.
2009,
Pubmed
Goldin,
Maintenance of Xenopus laevis and oocyte injection.
1992,
Pubmed
,
Xenbase
Gray,
Rapid redistribution of synaptic PSD-95 in the neocortex in vivo.
2006,
Pubmed
Grinvald,
VSDI: a new era in functional imaging of cortical dynamics.
2004,
Pubmed
Jin,
Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe.
2012,
Pubmed
Katayama,
GFP-like proteins stably accumulate in lysosomes.
2008,
Pubmed
Kohout,
Subunit organization and functional transitions in Ci-VSP.
2008,
Pubmed
Kondo,
A high GluR1 : GluR2 expression ratio is correlated with expression of Ca2+-binding proteins in rat forebrain neurons.
2000,
Pubmed
Kralj,
Optical recording of action potentials in mammalian neurons using a microbial rhodopsin.
2011,
Pubmed
Lam,
Improving FRET dynamic range with bright green and red fluorescent proteins.
2012,
Pubmed
Matsuda,
Direct measurement of protein dynamics inside cells using a rationally designed photoconvertible protein.
2008,
Pubmed
Murata,
Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor.
2005,
Pubmed
,
Xenbase
Ormö,
Crystal structure of the Aequorea victoria green fluorescent protein.
1996,
Pubmed
Perron,
Red-shifted voltage-sensitive fluorescent proteins.
2009,
Pubmed
Saito,
In vivo electroporation in the embryonic mouse central nervous system.
2006,
Pubmed
Saito,
Efficient gene transfer into the embryonic mouse brain using in vivo electroporation.
2001,
Pubmed
Sakai,
Design and characterization of a DNA-encoded, voltage-sensitive fluorescent protein.
2001,
Pubmed
Sawano,
Directed evolution of green fluorescent protein by a new versatile PCR strategy for site-directed and semi-random mutagenesis.
2000,
Pubmed
Shimogori,
Gene application with in utero electroporation in mouse embryonic brain.
2008,
Pubmed
Siegel,
A genetically encoded optical probe of membrane voltage.
1997,
Pubmed
,
Xenbase
Takahashi,
Transcranial fluorescence imaging of auditory cortical plasticity regulated by acoustic environments in mice.
2006,
Pubmed
Tanaka,
Construction of Epstein-Barr virus-based expression vector containing mini-oriP.
1999,
Pubmed
Tsutsui,
Visualizing voltage dynamics in zebrafish heart.
2010,
Pubmed
Tsutsui,
Optically detected structural change in the N-terminal region of the voltage-sensor domain.
2013,
Pubmed
Tsutsui,
Improving membrane voltage measurements using FRET with new fluorescent proteins.
2008,
Pubmed
Wall,
The structural basis for red fluorescence in the tetrameric GFP homolog DsRed.
2000,
Pubmed
Wilt,
Photon shot noise limits on optical detection of neuronal spikes and estimation of spike timing.
2013,
Pubmed
Xia,
Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism.
1996,
Pubmed
