Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Plant Physiol
2002 Feb 01;1282:564-77. doi: 10.1104/pp.010529.
Show Gene links
Show Anatomy links
A grapevine gene encoding a guard cell K(+) channel displays developmental regulation in the grapevine berry.
Pratelli R
,
Lacombe B
,
Torregrosa L
,
Gaymard F
,
Romieu C
,
Thibaud JB
,
Sentenac H
.
???displayArticle.abstract???
SIRK is a K(+) channel identified in grapevine (Vitis vinifera), belonging to the so-called Shaker family. The highest sequence similarities it shares with the members of this family are found with channels of the KAT type, although SIRK displays a small ankyrin domain. This atypical feature provides a key to understand the evolution of the plant Shaker family. Expression in Xenopus laevis oocytes indicated that SIRK is an inwardly rectifying channel displaying functional properties very similar to those of KAT2. The activity of SIRK promoter region fused to the GUS reporter gene was analyzed in both grapevine and Arabidopsis. Like other KAT-like channels, SIRK is expressed in guard cells. In Arabidopsis, the construct is also expressed in xylem parenchyma. Semiquantitative reverse transcriptase-polymerase chain reaction experiments indicated that SIRK transcript was present at low levels in the berry, during the first stages of berry growth. After veraison, the period of berry development that corresponds to the inception of ripening and that is associated with large biochemical and structural modifications, such as evolution of stomata in nonfunctional lenticels and degeneration of xylem vasculature, the transcript was no longer detected. The whole set of data suggests that in the berries SIRK is expressed in guard cells and, possibly, in xylem tissues. The encoded channel polypeptide could therefore play a role in the regulation of transpiration and water fluxes in grapevine fruits.
,
,
Pubmed
,
,
Pubmed
Ache,
GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K(+)-selective, K(+)-sensing ion channel.
2000,
Pubmed
,
Xenbase
Anderson,
Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae.
1992,
Pubmed
Becker,
Changes in voltage activation, Cs+ sensitivity, and ion permeability in H5 mutants of the plant K+ channel KAT1.
1996,
Pubmed
,
Xenbase
Bezanilla,
The voltage sensor in voltage-dependent ion channels.
2000,
Pubmed
Brüggemann,
Ether-à-go-go encodes a voltage-gated channel permeable to K+ and Ca2+ and modulated by cAMP.
1993,
Pubmed
,
Xenbase
Chérel,
Plant K+ channels: structure, activity and function.
1996,
Pubmed
Church,
Genomic sequencing.
1984,
Pubmed
Clough,
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
1998,
Pubmed
Daram,
Tetramerization of the AKT1 plant potassium channel involves its C-terminal cytoplasmic domain.
1997,
Pubmed
Davies,
Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins.
2000,
Pubmed
Davies,
Sugar accumulation in grape berries. Cloning of two putative vacuolar invertase cDNAs and their expression in grapevine tissues.
1996,
Pubmed
Fillion,
Cloning and expression of a hexose transporter gene expressed during the ripening of grape berry.
1999,
Pubmed
Gaymard,
Identification and disruption of a plant shaker-like outward channel involved in K+ release into the xylem sap.
1998,
Pubmed
,
Xenbase
Gonnet,
Exhaustive matching of the entire protein sequence database.
1992,
Pubmed
Hanley,
Plant intron sequences: evidence for distinct groups of introns.
1988,
Pubmed
Hawkins,
A survey on intron and exon lengths.
1988,
Pubmed
Hirsch,
A role for the AKT1 potassium channel in plant nutrition.
1998,
Pubmed
Hoffman,
New role found for a common protein "motif".
1991,
Pubmed
Jan,
Cloned potassium channels from eukaryotes and prokaryotes.
1997,
Pubmed
Jefferson,
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
1987,
Pubmed
Lacombe,
A shaker-like K(+) channel with weak rectification is expressed in both source and sink phloem tissues of Arabidopsis.
2000,
Pubmed
,
Xenbase
Lacombe,
pH control of the plant outwardly-rectifying potassium channel SKOR.
2000,
Pubmed
,
Xenbase
Lacombe,
Evidence for a multi-ion pore behavior in the plant potassium channel KAT1.
1998,
Pubmed
,
Xenbase
Lagarde,
Tissue-specific expression of Arabidopsis AKT1 gene is consistent with a role in K+ nutrition.
1996,
Pubmed
Li,
Factors influencing Agrobacterium-mediated transient expression of gusA in rice.
1992,
Pubmed
Lodhi,
Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae.
1995,
Pubmed
Lux,
Analysis of cDNA for human erythrocyte ankyrin indicates a repeated structure with homology to tissue-differentiation and cell-cycle control proteins.
1990,
Pubmed
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
Marck,
'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers.
1988,
Pubmed
Marten,
AKT3, a phloem-localized K+ channel, is blocked by protons.
1999,
Pubmed
,
Xenbase
Morsomme,
The plant plasma membrane H(+)-ATPase: structure, function and regulation.
2000,
Pubmed
Mouline,
Pollen tube development and competitive ability are impaired by disruption of a Shaker K(+) channel in Arabidopsis.
2002,
Pubmed
Müller-Röber,
Cloning and electrophysiological analysis of KST1, an inward rectifying K+ channel expressed in potato guard cells.
1995,
Pubmed
,
Xenbase
Nakamura,
Expression of an Arabidopsis potassium channel gene in guard cells.
1995,
Pubmed
Pilot,
Guard cell inward K+ channel activity in arabidopsis involves expression of the twin channel subunits KAT1 and KAT2.
2001,
Pubmed
,
Xenbase
Robinson,
A class IV chitinase is highly expressed in grape berries during ripening.
1997,
Pubmed
Ros,
The plant inorganic pyrophosphatase does not transport K+ in vacuole membrane vesicles multilabeled with fluorescent probes for H+, K+, and membrane potential.
1995,
Pubmed
Schachtman,
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
1992,
Pubmed
,
Xenbase
Sentenac,
Cloning and expression in yeast of a plant potassium ion transport system.
1992,
Pubmed
Spalding,
Potassium uptake supporting plant growth in the absence of AKT1 channel activity: Inhibition by ammonium and stimulation by sodium.
1999,
Pubmed
Szyroki,
KAT1 is not essential for stomatal opening.
2001,
Pubmed
Tattersall,
Identification and characterization of a fruit-specific, thaumatin-like protein that accumulates at very high levels in conjunction with the onset of sugar accumulation and berry softening in grapes.
1997,
Pubmed
Terrier,
Changes in acidity and in proton transport at the tonoplast of grape berries during development.
2001,
Pubmed
Uozumi,
Determination of transmembrane topology of an inward-rectifying potassium channel from Arabidopsis thaliana based on functional expression in Escherichia coli.
1998,
Pubmed
Urbach,
Biochemical characterization of the Arabidopsis K+ channels KAT1 and AKT1 expressed or co-expressed in insect cells.
2000,
Pubmed
,
Xenbase
Véry,
Expression of a cloned plant K+ channel in Xenopus oocytes: analysis of macroscopic currents.
1995,
Pubmed
,
Xenbase
Véry,
Level of expression in Xenopus oocytes affects some characteristics of a plant inward-rectifying voltage-gated K+ channel.
1994,
Pubmed
,
Xenbase
Zei,
Voltage-dependent gating of single wild-type and S4 mutant KAT1 inward rectifier potassium channels.
1998,
Pubmed
,
Xenbase
Zimmermann,
Plant ion channels: from molecular structures to physiological functions.
1999,
Pubmed
