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Proc Natl Acad Sci U S A
1993 May 15;9010:4758-62.
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Gating currents of the cloned delayed-rectifier K+ channel DRK1.
Taglialatela M
,
Stefani E
.
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Gating currents of the cloned delayed-rectifier K+ channel DRK1 expressed in Xenopus oocytes were measured with the open-oocyte Vaseline-gap voltage-clamp technique. DRK1 gating charge had the following salient properties: (i) gating-charge amplitude correlated positively with size of the expressed ionic K+ currents; (ii) the time integral of ON and OFF gating currents was similar, indicating charge conservation and lack of charge immobilization; (iii) the gating-charge activation curve was shallower and had a half-activation potential 15 mV more negative than the activation curve for K+ conductance; (iv) effective valence for the gating current was about two electronic charges per gating subunit; (v) for large depolarizations (to > 0 mV) prominent rising phases were observed during the ON and OFF gating charge, which appeared as shoulders in unsubtracted traces; (vi) for small depolarizing pulses (to < 0 mV) ionic-current activation and deactivation had time constants similar to ON and OFF gating-current decay, respectively; (vii) negative prepulses made more prominent the ON rising phase and delayed ionic and gating currents. The results are consistent with a model for K+ channel activation that has an early slow and/or weakly voltage-dependent transition between early closed states followed by more voltage-dependent transitions between later closed states and a final voltage-independent closed-open transition.
Almers,
Gating currents and charge movements in excitable membranes.
1978, Pubmed
Almers,
Gating currents and charge movements in excitable membranes.
1978,
Pubmed
Almers,
Survival of K+ permeability and gating currents in squid axons perfused with K+-free media.
1980,
Pubmed
Armstrong,
Currents related to movement of the gating particles of the sodium channels.
1973,
Pubmed
Armstrong,
Inactivation of the sodium channel. II. Gating current experiments.
1977,
Pubmed
Armstrong,
Time course of TEA(+)-induced anomalous rectification in squid giant axons.
1966,
Pubmed
Bezanilla,
Kinetic properties and inactivation of the gating currents of sodium channels in squid axon.
1975,
Pubmed
Bezanilla,
Molecular basis of gating charge immobilization in Shaker potassium channels.
1991,
Pubmed
,
Xenbase
Frech,
A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning.
1989,
Pubmed
,
Xenbase
Hartmann,
Exchange of conduction pathways between two related K+ channels.
1991,
Pubmed
,
Xenbase
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
HODGKIN,
The effect of sodium ions on the electrical activity of giant axon of the squid.
1949,
Pubmed
Kirsch,
Differences between the deep pores of K+ channels determined by an interacting pair of nonpolar amino acids.
1992,
Pubmed
,
Xenbase
Kirsch,
Internal and external TEA block in single cloned K+ channels.
1991,
Pubmed
,
Xenbase
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
Meves,
Slow recovery of sodium current and 'gating current' from inactivation.
1977,
Pubmed
Perozo,
Gating currents in Shaker K+ channels. Implications for activation and inactivation models.
1992,
Pubmed
,
Xenbase
Schoppa,
The size of gating charge in wild-type and mutant Shaker potassium channels.
1992,
Pubmed
Spires,
Pharmacological and kinetic analysis of K channel gating currents.
1989,
Pubmed
Stühmer,
Gating currents of inactivating and non-inactivating potassium channels expressed in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Taglialatela,
Patterns of internal and external tetraethylammonium block in four homologous K+ channels.
1991,
Pubmed
,
Xenbase
Taglialatela,
Novel voltage clamp to record small, fast currents from ion channels expressed in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
TAYLOR,
Analysis of certain errors in squid axon voltage clamp measurements.
1960,
Pubmed
Tytgat,
Evidence for cooperative interactions in potassium channel gating.
1992,
Pubmed
,
Xenbase
VanDongen,
Alteration and restoration of K+ channel function by deletions at the N- and C-termini.
1990,
Pubmed
White,
Activation of squid axon K+ channels. Ionic and gating current studies.
1985,
Pubmed
Yellen,
Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel.
1991,
Pubmed
Zagotta,
Voltage-dependent gating of Shaker A-type potassium channels in Drosophila muscle.
1990,
Pubmed
