Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
2005 Jul 12;10228:9954-9. doi: 10.1073/pnas.0504332102.
Show Gene links
Show Anatomy links
Phosphorylation-regulated endoplasmic reticulum retention signal in the renal outer-medullary K+ channel (ROMK).
O'Connell AD
,
Leng Q
,
Dong K
,
MacGregor GG
,
Giebisch G
,
Hebert SC
.
???displayArticle.abstract???
The renal outer-medullary K+ channel (ROMK; Kir1.1) mediates K+ secretion in the renal mammalian nephron that is critical to both sodium and potassium homeostasis. The posttranscriptional expression of ROMK in the plasma membrane of cells is regulated by delivery of protein from endoplasmic reticulum (ER) to the cell surface and by retrieval by dynamin-dependent endocytic mechanisms in clathrin-coated pits. The S44 in the NH(2) terminus of ROMK1 can be phosphorylated by PKA and serum- and glucocorticoid-inducible kinase-1, and this process increases surface expression of functional channels. We present evidence that phosphorylation of S44 modulates channel expression by increasing its cell surface delivery consequent to suppression of a COOH-terminal ER retention signal. This phosphorylation switch of the ER retention signal could provide a pool of mature and properly folded channels for rapid delivery to the plasma membrane. The x-ray crystal structures of inward rectifier K+ channels have shown a close apposition of the NH(2) terminus with the distal COOH terminus of the adjacent subunit in the channel homotetramer, which is important to channel gating. Thus, NH(2)-terminal phosphorylation modifying a COOH-terminal ER retention signal in ROMK1 could serve as a checkpoint for proper subunit folding critical to channel gating.
Ali,
The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels.
1998, Pubmed,
Xenbase
Ali,
The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels.
1998,
Pubmed
,
Xenbase
Boim,
ROMK inwardly rectifying ATP-sensitive K+ channel. II. Cloning and distribution of alternative forms.
1995,
Pubmed
,
Xenbase
Cabrera,
The retrieval function of the KDEL receptor requires PKA phosphorylation of its C-terminus.
2003,
Pubmed
Damke,
Induction of mutant dynamin specifically blocks endocytic coated vesicle formation.
1994,
Pubmed
Doyle,
Structural themes in ion channels.
2004,
Pubmed
Fujiwara,
Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum.
1988,
Pubmed
Giebisch,
Renal tubule potassium channels: function, regulation and structure.
2000,
Pubmed
Hebert,
Molecular diversity and regulation of renal potassium channels.
2005,
Pubmed
Ho,
Cloning and expression of an inwardly rectifying ATP-regulated potassium channel.
1993,
Pubmed
,
Xenbase
Hoover,
N-Glycosylation at two sites critically alters thiazide binding and activity of the rat thiazide-sensitive Na(+):Cl(-) cotransporter.
2003,
Pubmed
,
Xenbase
Huang,
Impaired regulation of renal K+ elimination in the sgk1-knockout mouse.
2004,
Pubmed
Kahle,
WNK4 regulates the balance between renal NaCl reabsorption and K+ secretion.
2003,
Pubmed
,
Xenbase
Kuo,
Crystal structure of the potassium channel KirBac1.1 in the closed state.
2003,
Pubmed
Lin,
ROMK1 channel activity is regulated by monoubiquitination.
2005,
Pubmed
,
Xenbase
Lin,
Protein kinase C (PKC)-induced phosphorylation of ROMK1 is essential for the surface expression of ROMK1 channels.
2002,
Pubmed
,
Xenbase
Lippincott-Schwartz,
Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic.
1991,
Pubmed
Lorenz,
Impaired renal NaCl absorption in mice lacking the ROMK potassium channel, a model for type II Bartter's syndrome.
2002,
Pubmed
Lu,
Absence of small conductance K+ channel (SK) activity in apical membranes of thick ascending limb and cortical collecting duct in ROMK (Bartter's) knockout mice.
2002,
Pubmed
Ma,
Role of ER export signals in controlling surface potassium channel numbers.
2001,
Pubmed
,
Xenbase
MacGregor,
Partially active channels produced by PKA site mutation of the cloned renal K+ channel, ROMK2 (kir1.2).
1998,
Pubmed
,
Xenbase
Malinow,
AMPA receptor trafficking and long-term potentiation.
2003,
Pubmed
Margeta-Mitrovic,
A trafficking checkpoint controls GABA(B) receptor heterodimerization.
2000,
Pubmed
,
Xenbase
Meade,
cAMP-dependent activation of the renal-specific Na+-K+-2Cl- cotransporter is mediated by regulation of cotransporter trafficking.
2003,
Pubmed
,
Xenbase
Palmada,
Negative charge at the consensus sequence for the serum- and glucocorticoid-inducible kinase, SGK1, determines pH sensitivity of the renal outer medullary K+ channel, ROMK1.
2003,
Pubmed
,
Xenbase
Palmada,
Molecular requirements for the regulation of the renal outer medullary K(+) channel ROMK1 by the serum- and glucocorticoid-inducible kinase SGK1.
2003,
Pubmed
,
Xenbase
Ren,
Cell surface expression of GluR5 kainate receptors is regulated by an endoplasmic reticulum retention signal.
2003,
Pubmed
Renault,
Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor.
2003,
Pubmed
Robinson,
The role of clathrin, adaptors and dynamin in endocytosis.
1994,
Pubmed
Scott,
An NMDA receptor ER retention signal regulated by phosphorylation and alternative splicing.
2001,
Pubmed
Scott,
Coordinated PKA and PKC phosphorylation suppresses RXR-mediated ER retention and regulates the surface delivery of NMDA receptors.
2003,
Pubmed
Shenolikar,
Regulation of ion transport by the NHERF family of PDZ proteins.
2004,
Pubmed
Sterling,
PKC expression is regulated by dietary K intake and mediates internalization of SK channels in the CCD.
2004,
Pubmed
van der Bliek,
Mutations in human dynamin block an intermediate stage in coated vesicle formation.
1993,
Pubmed
Wenthold,
Trafficking of NMDA receptors.
2003,
Pubmed
Xu,
Phosphorylation of the ATP-sensitive, inwardly rectifying K+ channel, ROMK, by cyclic AMP-dependent protein kinase.
1996,
Pubmed
,
Xenbase
Yoo,
Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A.
2003,
Pubmed
,
Xenbase
Yoo,
Assembly and trafficking of a multiprotein ROMK (Kir 1.1) channel complex by PDZ interactions.
2004,
Pubmed
,
Xenbase
Yun,
The serum and glucocorticoid-inducible kinase SGK1 and the Na+/H+ exchange regulating factor NHERF2 synergize to stimulate the renal outer medullary K+ channel ROMK1.
2002,
Pubmed
,
Xenbase
Zeng,
Evidence for endocytosis of ROMK potassium channel via clathrin-coated vesicles.
2002,
Pubmed
,
Xenbase
Zerangue,
A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
1999,
Pubmed
,
Xenbase
Zhou,
Phosphorylation and putative ER retention signals are required for protein kinase A-mediated potentiation of cardiac sodium current.
2002,
Pubmed
,
Xenbase
Zhou,
Primary structure and functional properties of an epithelial K channel.
1994,
Pubmed
,
Xenbase
