Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2009 Jun 19;28425:16716-22. doi: 10.1074/jbc.M109.011288.
Show Gene links
Show Anatomy links
Determinants of histone H4 N-terminal domain function during nucleosomal array oligomerization: roles of amino acid sequence, domain length, and charge density.
McBryant SJ
,
Klonoski J
,
Sorensen TC
,
Norskog SS
,
Williams S
,
Resch MG
,
Toombs JA
,
Hobdey SE
,
Hansen JC
.
???displayArticle.abstract???
Mg(2+)-dependent oligomerization of nucleosomal arrays is correlated with higher order folding transitions that stabilize chromosome structure beyond the 30-nm diameter fiber. In the present studies, we have employed a novel mutagenesis-based approach to identify the macromolecular determinants that control H4 N-terminal domain (NTD) function during oligomerization. Core histones were engineered in which 1) the H2A, H2B, and H3 NTDs were swapped onto the H4 histone fold; 2) the length of the H4 NTD and the H2A NTD on the H4 histone fold, were increased; 3) the charge density of the NTDs on the H4 histone fold was increased or decreased; and 4) the H4 NTD was placed on the H2B histone fold. Model nucleosomal arrays were assembled from wild type and mutant core histone octamers, and Mg(2+)-dependent oligomerization was characterized. The results demonstrated that the H2B and H3 NTDs could replace the H4 NTD, as could the H2A NTD if it was duplicated to the length of the native H4 NTD. Arrays oligomerized at lower salt concentrations as the length of the NTD on the H4 histone fold was increased. Mutations that decreased the NTD charge density required more Mg(2+) to oligomerize, whereas mutants that increased the charge density required less salt. Finally, the H4 NTD functioned differently when attached to the H2B histone fold than the H4 histone fold. These studies have revealed new insights into the biochemical basis for H4 NTD effects on genome architecture as well as the protein chemistry that underlies the function of the intrinsically disordered H4 NTD.
Aaronson,
Organization in the cell nucleus: divalent cations modulate the distribution of condensed and diffuse chromatin.
1981, Pubmed
Aaronson,
Organization in the cell nucleus: divalent cations modulate the distribution of condensed and diffuse chromatin.
1981,
Pubmed
Arya,
Flexible histone tails in a new mesoscopic oligonucleosome model.
2006,
Pubmed
Bártová,
Histone modifications and nuclear architecture: a review.
2008,
Pubmed
Baxevanis,
Histone Sequence Database: a compilation of highly-conserved nucleoprotein sequences.
1996,
Pubmed
Belmont,
Large-scale chromatin structural domains within mitotic and interphase chromosomes in vivo and in vitro.
1989,
Pubmed
Belmont,
Visualization of G1 chromosomes: a folded, twisted, supercoiled chromonema model of interphase chromatid structure.
1994,
Pubmed
Carruthers,
Sedimentation velocity analysis of macromolecular assemblies.
2000,
Pubmed
Carruthers,
The core histone N termini function independently of linker histones during chromatin condensation.
2000,
Pubmed
Chodaparambil,
A charged and contoured surface on the nucleosome regulates chromatin compaction.
2007,
Pubmed
Clark,
Electrostatic mechanism of chromatin folding.
1990,
Pubmed
Cosgrove,
How does the histone code work?
2005,
Pubmed
Demeler,
Sedimentation velocity analysis of highly heterogeneous systems.
2004,
Pubmed
Dixon,
The effects of sodium and magnesium-ion interactions on chromatin structure and solubility.
1985,
Pubmed
Dorigo,
Chromatin fiber folding: requirement for the histone H4 N-terminal tail.
2003,
Pubmed
,
Xenbase
Dunker,
Intrinsic disorder and protein function.
2002,
Pubmed
Dunker,
Intrinsically disordered protein.
2001,
Pubmed
Dyson,
Intrinsically unstructured proteins and their functions.
2005,
Pubmed
Fan,
The essential histone variant H2A.Z regulates the equilibrium between different chromatin conformational states.
2002,
Pubmed
,
Xenbase
Felsenfeld,
Controlling the double helix.
2003,
Pubmed
Fletcher,
The nucleosomal array: structure/function relationships.
1996,
Pubmed
Fletcher,
Core histone tail domains mediate oligonucleosome folding and nucleosomal DNA organization through distinct molecular mechanisms.
1995,
Pubmed
Fuxreiter,
Malleable machines take shape in eukaryotic transcriptional regulation.
2008,
Pubmed
Garcia-Ramirez,
Role of the histone "tails" in the folding of oligonucleosomes depleted of histone H1.
1992,
Pubmed
Garcia-Ramirez,
Modulation of chromatin folding by histone acetylation.
1995,
Pubmed
Gordon,
The core histone N-terminal tail domains function independently and additively during salt-dependent oligomerization of nucleosomal arrays.
2005,
Pubmed
,
Xenbase
Hansen,
Conformational dynamics of the chromatin fiber in solution: determinants, mechanisms, and functions.
2002,
Pubmed
Hansen,
Linking genome structure and function through specific histone acetylation.
2006,
Pubmed
Hansen,
Intrinsic protein disorder, amino acid composition, and histone terminal domains.
2006,
Pubmed
Hansen,
Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1.
1989,
Pubmed
Hansen,
Assembly and structural properties of subsaturated chromatin arrays.
1993,
Pubmed
Hansen,
Structure and function of the core histone N-termini: more than meets the eye.
1998,
Pubmed
Kan,
The H3 tail domain participates in multiple interactions during folding and self-association of nucleosome arrays.
2007,
Pubmed
,
Xenbase
Kan,
The H4 tail domain participates in intra- and internucleosome interactions with protein and DNA during folding and oligomerization of nucleosome arrays.
2009,
Pubmed
Krebs,
Moving marks: dynamic histone modifications in yeast.
2007,
Pubmed
Lu,
Chromatin condensing functions of the linker histone C-terminal domain are mediated by specific amino acid composition and intrinsic protein disorder.
2009,
Pubmed
Lu,
In vitro chromatin self-association and its relevance to genome architecture.
2006,
Pubmed
Lu,
The effect of H3K79 dimethylation and H4K20 trimethylation on nucleosome and chromatin structure.
2008,
Pubmed
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Preparation of nucleosome core particle from recombinant histones.
1999,
Pubmed
,
Xenbase
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Luger,
DNA binding within the nucleosome core.
1998,
Pubmed
Luger,
The histone tails of the nucleosome.
1998,
Pubmed
Luger,
Characterization of nucleosome core particles containing histone proteins made in bacteria.
1997,
Pubmed
,
Xenbase
Mészáros,
Molecular principles of the interactions of disordered proteins.
2007,
Pubmed
Moore,
Major role of the histones H3-H4 in the folding of the chromatin fiber.
1997,
Pubmed
Olins,
Physical studies of isolated eucaryotic nuclei.
1972,
Pubmed
Peterson,
Histones and histone modifications.
2004,
Pubmed
Pollard,
Functional interaction between GCN5 and polyamines: a new role for core histone acetylation.
1999,
Pubmed
Robinson,
30 nm chromatin fibre decompaction requires both H4-K16 acetylation and linker histone eviction.
2008,
Pubmed
,
Xenbase
Ross,
Scrambled prion domains form prions and amyloid.
2004,
Pubmed
Schwarz,
Reversible oligonucleosome self-association: dependence on divalent cations and core histone tail domains.
1996,
Pubmed
Schwarz,
Formation and stability of higher order chromatin structures. Contributions of the histone octamer.
1994,
Pubmed
Sharma,
Multiscale modeling of nucleosome dynamics.
2007,
Pubmed
Shilatifard,
Molecular implementation and physiological roles for histone H3 lysine 4 (H3K4) methylation.
2008,
Pubmed
Shoemaker,
Speeding molecular recognition by using the folding funnel: the fly-casting mechanism.
2000,
Pubmed
Shogren-Knaak,
Switching on chromatin: mechanistic role of histone H4-K16 acetylation.
2006,
Pubmed
Shogren-Knaak,
Histone H4-K16 acetylation controls chromatin structure and protein interactions.
2006,
Pubmed
Taverna,
How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
2007,
Pubmed
Tompa,
Fuzzy complexes: polymorphism and structural disorder in protein-protein interactions.
2008,
Pubmed
Tompa,
Intrinsically unstructured proteins.
2002,
Pubmed
Tremethick,
Higher-order structures of chromatin: the elusive 30 nm fiber.
2007,
Pubmed
Tse,
Hybrid trypsinized nucleosomal arrays: identification of multiple functional roles of the H2A/H2B and H3/H4 N-termini in chromatin fiber compaction.
1997,
Pubmed
Tse,
Disruption of higher-order folding by core histone acetylation dramatically enhances transcription of nucleosomal arrays by RNA polymerase III.
1998,
Pubmed
,
Xenbase
Turner,
Defining an epigenetic code.
2007,
Pubmed
Villar-Garea,
The analysis of histone modifications.
2006,
Pubmed
Wang,
Acetylation mimics within individual core histone tail domains indicate distinct roles in regulating the stability of higher-order chromatin structure.
2008,
Pubmed
,
Xenbase
Weith,
Mg2+-dependent compactness of heterochromatic chromosome segments.
1983,
Pubmed
Widom,
Structure, dynamics, and function of chromatin in vitro.
1998,
Pubmed
Zheng,
Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
2003,
Pubmed
,
Xenbase
Zhou,
The nucleosome surface regulates chromatin compaction and couples it with transcriptional repression.
2007,
Pubmed
