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Proc Natl Acad Sci U S A
1990 Dec 01;8723:9153-7. doi: 10.1073/pnas.87.23.9153.
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Recombinant yeast TFIID, a general transcription factor, mediates activation by the gene-specific factor USF in a chromatin assembly assay.
Meisterernst M
,
Horikoshi M
,
Roeder RG
.
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The TATA box-binding transcription factor TFIID from Saccharomyces cerevisiae was tested for its ability to mediate regulatory factor functions both in a cell-free system reconstituted with other general initiation factors (purified from HeLa cells) and in a combined nucleosome assembly-transcription system. In the latter assay recombinant yeast TFIID, expressed in and purified from bacteria, was sufficient to prevent nucleosome assembly-mediated repression and to mediate transcriptional enhancement of the adenovirus major late promoter by the gene-specific activator USF. In contrast, recombinant yeast TFIID was unable to mediate activation by USF in the system reconstituted only with purified general factors. Under the same conditions a partially purified natural yeast TFIID was able to mediate activation by both USF and Sp1 (assayed with the human immunodeficiency virus promoter), but to a lesser extent than observed with a partially purified natural human TFIID. The implications of these findings are discussed with respect to the structure of the yeast and human TATA factors and the possible involvement either of specific TFIID modifications or of coactivators.
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,
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,
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Gross,
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Han,
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Hoffman,
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Horikoshi,
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Horikoshi,
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Pubmed
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Jackson,
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Jones,
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Kao,
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,
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,
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1987,
Pubmed
Nakajima,
Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.
1988,
Pubmed
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Functional domains and upstream activation properties of cloned human TATA binding protein.
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Pubmed
Ptashne,
Activators and targets.
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Pubmed
Pugh,
Mechanism of transcriptional activation by Sp1: evidence for coactivators.
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Pubmed
Rosen,
The location of cis-acting regulatory sequences in the human T cell lymphotropic virus type III (HTLV-III/LAV) long terminal repeat.
1985,
Pubmed
Sawadogo,
Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.
1985,
Pubmed
Sawadogo,
Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
1985,
Pubmed
Stringer,
Direct and selective binding of an acidic transcriptional activation domain to the TATA-box factor TFIID.
1990,
Pubmed
Van Dyke,
Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
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Pubmed
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Assembly and propagation of repressed and depressed chromosomal states.
1985,
Pubmed
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Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly.
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Pubmed
,
Xenbase
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Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
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,
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Workman,
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,
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