Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Inhibition by ethanol of rat P2X(4) receptors expressed in Xenopus oocytes.
Xiong K
,
Li C
,
Weight FF
.
???displayArticle.abstract???
1. The effect of ethanol on the function of P2X(4) receptors expressed in Xenopus oocytes was studied using two-electrode voltage-clamp recording. 2. The amplitude of current activated by 1 microM ATP was decreased by ethanol in a concentration-dependent manner over the concentration range 1 - 500 mM. The concentration of ethanol that produced 50% inhibition (IC(50)) of current activated by 1 microM ATP was 58 mM. 3. Ethanol inhibition of ATP-activated current was not dependent on membrane potential from -60 to +20 mV, and ethanol did not change the reversal potential of ATP-activated current. 4. Ethanol, 50 mM, shifted the ATP concentration-response curve to the right, increasing the EC(50) for ATP from 9.1 to 16.0 microM, but did not reduce the maximal response to ATP. 5. The results suggest that ethanol may inhibit P2X(4) receptors by decreasing the apparent affinity of the binding site for ATP. 6. Since the P2X(4) receptor is the most abundant P2X subunit in the brain, these receptors could be important effectors of ethanol action in the central nervous system.
Bardoni,
ATP P2X receptors mediate fast synaptic transmission in the dorsal horn of the rat spinal cord.
1997, Pubmed
Bardoni,
ATP P2X receptors mediate fast synaptic transmission in the dorsal horn of the rat spinal cord.
1997,
Pubmed
Bean,
ATP-activated channels in rat and bullfrog sensory neurons: concentration dependence and kinetics.
1990,
Pubmed
Bo,
A P2X purinoceptor cDNA conferring a novel pharmacological profile.
1995,
Pubmed
Buell,
P2X receptors: an emerging channel family.
1996,
Pubmed
Buell,
An antagonist-insensitive P2X receptor expressed in epithelia and brain.
1996,
Pubmed
Cloues,
Zn2+ potentiates ATP-activated currents in rat sympathetic neurons.
1993,
Pubmed
Collo,
Cloning OF P2X5 and P2X6 receptors and the distribution and properties of an extended family of ATP-gated ion channels.
1996,
Pubmed
DeLean,
Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves.
1978,
Pubmed
Dhulipala,
The human P2X4 receptor gene is alternatively spliced.
1998,
Pubmed
,
Xenbase
Edwards,
ATP receptor-mediated synaptic currents in the central nervous system.
1992,
Pubmed
Edwards,
Properties of ATP receptor-mediated synaptic transmission in the rat medial habenula.
1997,
Pubmed
Evans,
ATP mediates fast synaptic transmission in mammalian neurons.
1992,
Pubmed
Fieber,
Adenosine triphosphate-evoked currents in cultured neurones dissociated from rat parasympathetic cardiac ganglia.
1991,
Pubmed
Galligan,
ATP mediates fast synaptic potentials in enteric neurons.
1994,
Pubmed
Garcia-Guzman,
Characterization of recombinant human P2X4 receptor reveals pharmacological differences to the rat homologue.
1997,
Pubmed
,
Xenbase
Khakh,
Electrophysiological properties of P2X-purinoceptors in rat superior cervical, nodose and guinea-pig coeliac neurones.
1995,
Pubmed
Krishtal,
Receptor for ATP in the membrane of mammalian sensory neurones.
1983,
Pubmed
Lê,
Central P2X4 and P2X6 channel subunits coassemble into a novel heteromeric ATP receptor.
1998,
Pubmed
,
Xenbase
Li,
Ethanol-induced inhibition of a neuronal P2X purinoceptor by an allosteric mechanism.
1998,
Pubmed
Li,
Inhibition of ATP-activated current by zinc in dorsal root ganglion neurones of bullfrog.
1997,
Pubmed
Li,
Ethanol inhibits a neuronal ATP-gated ion channel.
1993,
Pubmed
Li,
Zn2+ potentiates excitatory action of ATP on mammalian neurons.
1993,
Pubmed
MacKenzie,
Functional and molecular diversity of purinergic ion channel receptors.
1999,
Pubmed
Magleby,
The effect of voltage on the time course of end-plate currents.
1972,
Pubmed
Nakazawa,
ATP-activated current and its interaction with acetylcholine-activated current in rat sympathetic neurons.
1994,
Pubmed
Nieber,
Role of ATP in fast excitatory synaptic potentials in locus coeruleus neurones of the rat.
1997,
Pubmed
Pankratov,
A purinergic component of the excitatory postsynaptic current mediated by P2X receptors in the CA1 neurons of the rat hippocampus.
1998,
Pubmed
Peoples,
Ethanol inhibition of N-methyl-D-aspartate-activated current in mouse hippocampal neurones: whole-cell patch-clamp analysis.
1997,
Pubmed
Séguéla,
A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain.
1996,
Pubmed
,
Xenbase
Shen,
Excitation of rat locus coeruleus neurons by adenosine 5'-triphosphate: ionic mechanism and receptor characterization.
1993,
Pubmed
Silinsky,
ATP mediates excitatory synaptic transmission in mammalian neurones.
1992,
Pubmed
Soto,
P2X4: an ATP-activated ionotropic receptor cloned from rat brain.
1996,
Pubmed
,
Xenbase
Townsend-Nicholson,
Molecular cloning, functional characterization and possible cooperativity between the murine P2X4 and P2X4a receptors.
1999,
Pubmed
,
Xenbase
Tschöpl,
Excitatory effects of adenosine 5'-triphosphate on rat locus coeruleus neurones.
1992,
Pubmed
Ueno,
ATP-gated current in dissociated rat nucleus solitarii neurons.
1992,
Pubmed
Wang,
Cloning and pharmacological characterization of a fourth P2X receptor subtype widely expressed in brain and peripheral tissues including various endocrine tissues.
1996,
Pubmed
,
Xenbase
Weight,
Alcohol action on membrane ion channels gated by extracellular ATP (P2X receptors).
1999,
Pubmed
Xiong,
Differential modulation by copper and zinc of P2X2 and P2X4 receptor function.
1999,
Pubmed
,
Xenbase
Zhong,
Pharmacological and molecular characterization of P2X receptors in rat pelvic ganglion neurons.
1998,
Pubmed
