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Nat Neurosci
2004 Dec 01;712:1381-6. doi: 10.1038/nn1356.
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Light-activated ion channels for remote control of neuronal firing.
Banghart M
,
Borges K
,
Isacoff E
,
Trauner D
,
Kramer RH
.
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Neurons have ion channels that are directly gated by voltage, ligands and temperature but not by light. Using structure-based design, we have developed a new chemical gate that confers light sensitivity to an ion channel. The gate includes a functional group for selective conjugation to an engineered K(+) channel, a pore blocker and a photoisomerizable azobenzene. Long-wavelength light drives the azobenzene moiety into its extended trans configuration, allowing the blocker to reach the pore. Short-wavelength light generates the shorter cis configuration, retracting the blocker and allowing conduction. Exogenous expression of these channels in rat hippocampal neurons, followed by chemical modification with the photoswitchable gate, enables different wavelengths of light to switch action potential firing on and off. These synthetic photoisomerizable azobenzene-regulated K(+) (SPARK) channels allow rapid, precise and reversible control over neuronal firing, with potential applications for dissecting neural circuits and controlling activity downstream from sites of neural damage or degeneration.
Blaustein,
Kinetics of tethering quaternary ammonium compounds to K(+) channels.
2002, Pubmed,
Xenbase
Blaustein,
Kinetics of tethering quaternary ammonium compounds to K(+) channels.
2002,
Pubmed
,
Xenbase
Blaustein,
Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel.
2000,
Pubmed
,
Xenbase
Callaway,
Caged neurotransmitters. Shedding light on neural circuits.
1994,
Pubmed
Choi,
Tetraethylammonium blockade distinguishes two inactivation mechanisms in voltage-activated K+ channels.
1991,
Pubmed
Djurisic,
Optical monitoring of neural activity using voltage-sensitive dyes.
2003,
Pubmed
Doyle,
The structure of the potassium channel: molecular basis of K+ conduction and selectivity.
1998,
Pubmed
Flint,
Using an azobenzene cross-linker to either increase or decrease peptide helix content upon trans-to-cis photoisomerization.
2002,
Pubmed
Griffin,
Specific covalent labeling of recombinant protein molecules inside live cells.
1998,
Pubmed
Gu,
A conserved domain in axonal targeting of Kv1 (Shaker) voltage-gated potassium channels.
2003,
Pubmed
Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
James,
Kinetic characterization of ribonuclease S mutants containing photoisomerizable phenylazophenylalanine residues.
2001,
Pubmed
Jiang,
The open pore conformation of potassium channels.
2002,
Pubmed
Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed
Johns,
Inducible genetic suppression of neuronal excitability.
1999,
Pubmed
Karschin,
K+ channel expression in primary cell cultures mediated by vaccinia virus.
1991,
Pubmed
Katz,
Scanning laser photostimulation: a new approach for analyzing brain circuits.
1994,
Pubmed
Lechner,
A genetic method for selective and quickly reversible silencing of Mammalian neurons.
2002,
Pubmed
Lester,
A covalently bound photoisomerizable agonist: comparison with reversibly bound agonists at Electrophorus electroplaques.
1980,
Pubmed
Lopez,
Hydrophobic substitution mutations in the S4 sequence alter voltage-dependent gating in Shaker K+ channels.
1991,
Pubmed
,
Xenbase
López-Barneo,
Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels.
1993,
Pubmed
,
Xenbase
MacKinnon,
Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels.
1990,
Pubmed
Nerbonne,
Caged compounds: tools for illuminating neuronal responses and connections.
1996,
Pubmed
Nitabach,
Electrical silencing of Drosophila pacemaker neurons stops the free-running circadian clock.
2002,
Pubmed
Sigworth,
Voltage gating of ion channels.
1994,
Pubmed
Slimko,
Selective electrical silencing of mammalian neurons in vitro by the use of invertebrate ligand-gated chloride channels.
2002,
Pubmed
Sutherland,
Overexpression of a Shaker-type potassium channel in mammalian central nervous system dysregulates native potassium channel gene expression.
1999,
Pubmed
White,
Targeted attenuation of electrical activity in Drosophila using a genetically modified K(+) channel.
2001,
Pubmed
Yellen,
The voltage-gated potassium channels and their relatives.
2002,
Pubmed
Zemelman,
Selective photostimulation of genetically chARGed neurons.
2002,
Pubmed
,
Xenbase
