Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract??? XUBF is a Xenopus ribosomal transcription factor of the HMG-box family which contains five tandemly disposed homologies to the HMG1 & 2 DNA binding domains. XUBF has been isolated as a protein doublet and two cDNAs encoding the two molecular weight variants have been characterised. The major two forms of xUBF identified differ by the presence or absence of a 22 amino acid segment lying between HMG-boxes 3 and 4. Here we show that the mRNAs for these two forms of xUBF are regulated during development and differentiation over a range of nearly 20 fold. By isolating two of the xUBF genes, it was possible to show that both encoded the variable 22 amino acid segment in exon 12. Oocyte splicing assays and the sequencing of PCR-generated cDNA fragments, demonstrated that the transcripts from one of these genes were differentially spliced in a developmentally regulated manner. Transcripts from the second gene were found to be predominantly or exclusively spliced to produce the lower molecular weight form of xUBF. Expression of a high molecular weight form from yet a third gene was also detected. Although the intron-exon structures of the Xenopus and mouse UBF genes were found to be essentially identical, the differential splicing of exon 8 found in mammals, was not detected in Xenopus.
Bachvarov,
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
1991, Pubmed,
Xenbase
Bachvarov,
The RNA polymerase I transcription factor xUBF contains 5 tandemly repeated HMG homology boxes.
1991,
Pubmed
,
Xenbase
Bachvarov,
Heterogeneity in the Xenopus ribosomal transcription factor xUBF has a molecular basis distinct from that in mammals.
1991,
Pubmed
,
Xenbase
Bell,
Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.
1990,
Pubmed
Bell,
Molecular mechanisms governing species-specific transcription of ribosomal RNA.
1989,
Pubmed
,
Xenbase
Cathala,
A method for isolation of intact, translationally active ribonucleic acid.
1983,
Pubmed
Comai,
The TATA-binding protein and associated factors are integral components of the RNA polymerase I transcription factor, SL1.
1992,
Pubmed
Del Sal,
The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing.
1989,
Pubmed
De Winter,
Spacer promoters are essential for efficient enhancement of X. laevis ribosomal transcription.
1986,
Pubmed
,
Xenbase
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Gubbay,
A gene mapping to the sex-determining region of the mouse Y chromosome is a member of a novel family of embryonically expressed genes.
1990,
Pubmed
Hisatake,
Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF.
1991,
Pubmed
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Kawasaki,
Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro.
1988,
Pubmed
Kolodrubetz,
Duplicated NHP6 genes of Saccharomyces cerevisiae encode proteins homologous to bovine high mobility group protein 1.
1990,
Pubmed
Kolodrubetz,
Consensus sequence for HMG1-like DNA binding domains.
1990,
Pubmed
Krieg,
Improved synthesis of full-length RNA probe at reduced incubation temperatures.
1990,
Pubmed
Krieg,
In vitro RNA synthesis with SP6 RNA polymerase.
1987,
Pubmed
McStay,
xUBF and Rib 1 are both required for formation of a stable polymerase I promoter complex in X. laevis.
1991,
Pubmed
,
Xenbase
Mishima,
Fractionation and reconstitution of factors required for accurate transcription of mammalian ribosomal RNA genes: identification of a species-dependent initiation factor.
1982,
Pubmed
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
O'Mahony,
Identification of two forms of the RNA polymerase I transcription factor UBF.
1991,
Pubmed
Parisi,
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
1991,
Pubmed
Pikaard,
rUBF, an RNA polymerase I transcription factor from rats, produces DNase I footprints identical to those produced by xUBF, its homolog from frogs.
1990,
Pubmed
,
Xenbase
Pikaard,
The Xenopus ribosomal gene enhancers bind an essential polymerase I transcription factor, xUBF.
1989,
Pubmed
,
Xenbase
Rappolee,
Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos.
1988,
Pubmed
Rappolee,
Wound macrophages express TGF-alpha and other growth factors in vivo: analysis by mRNA phenotyping.
1988,
Pubmed
Reeves,
The A.T-DNA-binding domain of mammalian high mobility group I chromosomal proteins. A novel peptide motif for recognizing DNA structure.
1990,
Pubmed
Riley,
Very rapid nucleotide sequence analysis of improved, double-stranded minipreps.
1989,
Pubmed
Schnapp,
Trans-acting factors involved in species-specificity and control of mouse ribosomal gene transcription.
,
Pubmed
Schnapp,
Isolation and functional characterization of TIF-IB, a factor that confers promoter specificity to mouse RNA polymerase I.
1990,
Pubmed
Sinclair,
A gene from the human sex-determining region encodes a protein with homology to a conserved DNA-binding motif.
1990,
Pubmed
Smith,
Characterization of factors that direct transcription of rat ribosomal DNA.
1990,
Pubmed
Tower,
Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.
1986,
Pubmed
Travis,
LEF-1, a gene encoding a lymphoid-specific protein with an HMG domain, regulates T-cell receptor alpha enhancer function [corrected].
1991,
Pubmed
