Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
If RNA editing could be rationally directed to mutated RNA sequences, genetic diseases caused by certain base substitutions could be treated. Here we use a synthetic complementary RNA oligonucleotide to direct the correction of a premature stop codon mutation in dystrophin RNA. The complementary RNA oligonucleotide was hybridized to a premature stop codon and the hybrid was treated with nuclear extracts containing the cellular enzyme double-stranded RNA adenosine deaminase. When the treated RNAs were translated in vitro, a dramatic increase in expression of a downstream luciferase coding region was observed. The cDNA sequence data are consistent with deamination of the adenosine in the UAG stop codon to inosine by double-stranded RNA adenosine deaminase. Injection of oligonucleotide-mRNA hybrids into Xenopus embryos also resulted in an increase in luciferase expression. These experiments demonstrate the principle of therapeutic RNA editing.
Backus,
Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited.
1994, Pubmed
Backus,
Only cytidines 5' of the apolipoprotein B mRNA mooring sequence are edited.
1994,
Pubmed
Bartel,
Isolation of new ribozymes from a large pool of random sequences [see comment].
1993,
Pubmed
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Bass,
An unwinding activity that covalently modifies its double-stranded RNA substrate.
1988,
Pubmed
,
Xenbase
Blum,
A model for RNA editing in kinetoplastid mitochondria: "guide" RNA molecules transcribed from maxicircle DNA provide the edited information.
1990,
Pubmed
Bulman,
Point mutation in the human dystrophin gene: identification through western blot analysis.
1991,
Pubmed
Dai,
Cleavage of an amide bond by a ribozyme.
1995,
Pubmed
Dominski,
Restoration of correct splicing in thalassemic pre-mRNA by antisense oligonucleotides.
1993,
Pubmed
Higuchi,
RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency.
1993,
Pubmed
Lamond,
2'-O-alkyloligoribonucleotides: probes for studying the biochemistry and cell biology of RNA processing.
1993,
Pubmed
Maher,
Comparative hybrid arrest by tandem antisense oligodeoxyribonucleotides or oligodeoxyribonucleoside methylphosphonates in a cell-free system.
1988,
Pubmed
Melton,
Injected anti-sense RNAs specifically block messenger RNA translation in vivo.
1985,
Pubmed
,
Xenbase
Monia,
Selective inhibition of mutant Ha-ras mRNA expression by antisense oligonucleotides.
1992,
Pubmed
Navaratnam,
The p27 catalytic subunit of the apolipoprotein B mRNA editing enzyme is a cytidine deaminase.
1993,
Pubmed
,
Xenbase
Polson,
Preferential selection of adenosines for modification by double-stranded RNA adenosine deaminase.
1994,
Pubmed
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Roberts,
Searching for the 1 in 2,400,000: a review of dystrophin gene point mutations.
1994,
Pubmed
Saccomanno,
The cytoplasm of Xenopus oocytes contains a factor that protects double-stranded RNA from adenosine-to-inosine modification.
1994,
Pubmed
,
Xenbase
Scaringe,
Chemical synthesis of biologically active oligoribonucleotides using beta-cyanoethyl protected ribonucleoside phosphoramidites.
1990,
Pubmed
Simpson,
RNA editing--a novel genetic phenomenon?
1990,
Pubmed
Sullenger,
Ribozyme-mediated repair of defective mRNA by targeted, trans-splicing.
1994,
Pubmed
Wagner,
A double-stranded RNA unwinding activity introduces structural alterations by means of adenosine to inosine conversions in mammalian cells and Xenopus eggs.
1989,
Pubmed
,
Xenbase
