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Biochemistry
2010 Feb 02;494:653-5. doi: 10.1021/bi902155t.
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Functional delivery of a membrane protein into oocyte membranes using bicelles.
Kang C
,
Vanoye CG
,
Welch RC
,
Van Horn WD
,
Sanders CR
.
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Voltage-gated potassium channel modulatory membrane protein KCNE3 was overexpressed and purified into both micelles and bicelles. Remarkably, microinjection of KCNE3 in bicelles into Xenopus oocytes resulted in functional co-assembly with the human KCNQ1 channel expressed therein. Microinjection of LMPC micelles containing KCNE3 did not result in channel modulation, indicating that bicelles sometimes succeed at delivering a membrane protein into a cellular membrane when classical micelles fail. Backbone NMR resonance assignments were completed for KCNE3 in both bicelles and LMPC, indicating that the secondary structure distribution in KCNE3's N-terminus and transmembrane domains exhibits only modest differences from that of KCNE1, even though these KCNE family members have very different effects on KCNQ1 channel function.
Abbott,
Phosphorylation and protonation of neighboring MiRP2 sites: function and pathophysiology of MiRP2-Kv3.4 potassium channels in periodic paralysis.
2006, Pubmed
Abbott,
Phosphorylation and protonation of neighboring MiRP2 sites: function and pathophysiology of MiRP2-Kv3.4 potassium channels in periodic paralysis.
2006,
Pubmed
Anantharam,
RNA interference reveals that endogenous Xenopus MinK-related peptides govern mammalian K+ channel function in oocyte expression studies.
2003,
Pubmed
,
Xenbase
Chen,
Functional interactions between KCNE1 C-terminus and the KCNQ1 channel.
2009,
Pubmed
Chen,
Charybdotoxin binding in the I(Ks) pore demonstrates two MinK subunits in each channel complex.
2003,
Pubmed
,
Xenbase
Choi,
The MiRP2-Kv3.4 potassium channel: muscling in on Alzheimer's disease.
2007,
Pubmed
Delpón,
Functional effects of KCNE3 mutation and its role in the development of Brugada syndrome.
2008,
Pubmed
Gage,
KCNE3 truncation mutants reveal a bipartite modulation of KCNQ1 K+ channels.
2004,
Pubmed
,
Xenbase
Goncharuk,
Bacterial synthesis, purification, and solubilization of membrane protein KCNE3, a regulator of voltage-gated potassium channels.
2009,
Pubmed
Grahammer,
The cardiac K+ channel KCNQ1 is essential for gastric acid secretion.
2001,
Pubmed
Grahammer,
The small conductance K+ channel, KCNQ1: expression, function, and subunit composition in murine trachea.
2001,
Pubmed
Heitzmann,
KCNE beta subunits determine pH sensitivity of KCNQ1 potassium channels.
2007,
Pubmed
Jespersen,
The KCNQ1 potassium channel: from gene to physiological function.
2005,
Pubmed
Kang,
Structure of KCNE1 and implications for how it modulates the KCNQ1 potassium channel.
2008,
Pubmed
Lundby,
KCNE3 mutation V17M identified in a patient with lone atrial fibrillation.
2008,
Pubmed
,
Xenbase
McCrossan,
The MinK-related peptides.
2004,
Pubmed
,
Xenbase
Melman,
Structural determinants of KvLQT1 control by the KCNE family of proteins.
2001,
Pubmed
Melman,
A single transmembrane site in the KCNE-encoded proteins controls the specificity of KvLQT1 channel gating.
2002,
Pubmed
Morin,
Counting membrane-embedded KCNE beta-subunits in functioning K+ channel complexes.
2008,
Pubmed
,
Xenbase
Ohno,
Novel KCNE3 mutation reduces repolarizing potassium current and associated with long QT syndrome.
2009,
Pubmed
Peretz,
Modulation of homomeric and heteromeric KCNQ1 channels by external acidification.
2002,
Pubmed
,
Xenbase
Prosser,
Current applications of bicelles in NMR studies of membrane-associated amphiphiles and proteins.
2006,
Pubmed
Rocheleau,
Secondary structure of a KCNE cytoplasmic domain.
2006,
Pubmed
,
Xenbase
Sanders,
Bicelles: a model membrane system for all seasons?
1998,
Pubmed
Sanders,
French swimwear for membrane proteins.
2004,
Pubmed
Schroeder,
A constitutively open potassium channel formed by KCNQ1 and KCNE3.
2000,
Pubmed
,
Xenbase
Shen,
TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts.
2009,
Pubmed
Splawski,
Mutations in the hminK gene cause long QT syndrome and suppress IKs function.
1997,
Pubmed
,
Xenbase
Tapper,
MinK subdomains that mediate modulation of and association with KvLQT1.
2000,
Pubmed
,
Xenbase
Tian,
Preparation, functional characterization, and NMR studies of human KCNE1, a voltage-gated potassium channel accessory subunit associated with deafness and long QT syndrome.
2007,
Pubmed
,
Xenbase
