XB-ART-36566
Nat Methods
2007 Sep 01;49:741-6. doi: 10.1038/nmeth1083.
Show Gene links
Show Anatomy links
Bright far-red fluorescent protein for whole-body imaging.
???displayArticle.abstract???
For deep imaging of animal tissues, the optical window favorable for light penetration is in near-infrared wavelengths, which requires proteins with emission spectra in the far-red wavelengths. Here we report a far-red fluorescent protein, named Katushka, which is seven- to tenfold brighter compared to the spectrally close HcRed or mPlum, and is characterized by fast maturation as well as a high pH-stability and photostability. These unique characteristics make Katushka the protein of choice for visualization in living tissues. We demonstrate superiority of Katushka for whole-body imaging by direct comparison with other red and far-red fluorescent proteins. We also describe a monomeric version of Katushka, named mKate, which is characterized by high brightness and photostability, and should be an excellent fluorescent label for protein tagging in the far-red part of the spectrum.
???displayArticle.pubmedLink??? 17721542
???displayArticle.link??? Nat Methods
???displayArticle.grants???
Species referenced: Xenopus laevis