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Proc Natl Acad Sci U S A
1991 Oct 01;8819:8397-400.
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Coexpression of alpha 1 with putative beta 3 subunits results in functional Na+/K+ pumps in Xenopus oocytes.
Horisberger JD
,
Jaunin P
,
Good PJ
,
Rossier BC
,
Geering K
.
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The active Na+/K+ pump is composed of an alpha and a beta subunit. Until now, three putative isoforms of the beta subunit have been identified that share sequence similarity. We have expressed the beta 1 and beta 3 isoforms of Xenopus laevis Na+/K(+)-ATPase in Xenopus oocytes to compare functional properties of the Na+/K+ pump, including either of these two isoforms. Na+/K+ pump current, estimated as K(+)-induced outward current in voltage-clamped oocytes, was doubled by coexpression of alpha 1 subunits with either isoform of the beta subunit compared to expression of alpha 1 subunits alone. The kinetics of activation by external K+ and the voltage dependence of the electrogenic activity of the Na+/K+ pump were similar with both beta isoforms, indicating that both beta 1 and beta 3 isoforms can support expression at the oocyte surface of an active Na+/K+ pump with similar functional properties.
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,
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Geering,
Subunit assembly and functional maturation of Na,K-ATPase.
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Geering,
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,
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Good,
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Good,
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,
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Structure-function relationship of Na,K-ATPase.
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Horisberger,
Na-K pump current in the Amphiuma collecting tubule.
1989,
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Latorre,
Conduction and selectivity in potassium channels.
1983,
Pubmed
Läuger,
Dynamics of ion transport systems in membranes.
1987,
Pubmed
Martin-Vasallo,
Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression.
1989,
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Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
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Noguchi,
Expression of functional (Na+ + K+)-ATPase from cloned cDNAs.
1987,
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,
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Rakowski,
A negative slope in the current-voltage relationship of the Na+/K+ pump in Xenopus oocytes produced by reduction of external [K+].
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,
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Rakowski,
Voltage dependence of Na/K pump current in Xenopus oocytes.
1988,
Pubmed
,
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Gene expression in the embryonic nervous system of Xenopus laevis.
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,
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Schwarz,
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,
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Shyjan,
The Na,K-ATPase beta 2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity.
1990,
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Verrey,
Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells.
1989,
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,
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