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To examine the role of primary amino acid sequence in the localization of proteins within the nucleus, we studied the nucleolar protein NO38 of amphibian oocytes. We synthesized NO38 transcripts in vitro, injected them into the oocytecytoplasm, and followed the distribution of the translation products. The injected RNA contained a short sequence encoding an epitope derived from the human c-myc protein. We used an mAb against this epitope to detect translation products from injected RNAs by Western blots and by immunofluoresent staining of cytological preparations. When full-length transcripts of NO38 were injected into oocytes, the translation products accumulated efficiently in the germinal vesicle, and a major fraction was localized in the multiple nucleoli. To identify protein domains involved in this nucleolus-specific accumulation, we prepared a series of carboxy-terminal deletions of the cDNA. Oocytes injected with RNA encoding truncated forms of NO38 were examined for altered patterns of protein accumulation. We defined a domain of about 24 amino acids near the carboxy terminus that was essential for nucleolar localization of NO38. This domain is separated by more than 70 amino acids from two putative nuclear localization signals near the middle of the molecule. Hybrid constructs were made which encoded part of Escherichia coli beta-galactosidase or pyruvate kinase fused to a long segment of NO38 containing the essential domain. Injection of RNA from these constructs showed that the essential domain was not sufficient to target the hybrid proteins to the nucleolus. We suggest that nucleolar accumulation of NO38 requires more than a single linear domain.
Benavente,
Karyoskeletal proteins and the organization of the amphibian oocyte nucleus.
1984, Pubmed,
Xenbase
Benavente,
Karyoskeletal proteins and the organization of the amphibian oocyte nucleus.
1984,
Pubmed
,
Xenbase
Bonner,
Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins.
1975,
Pubmed
,
Xenbase
Bürglin,
The nuclear migration signal of Xenopus laevis nucleoplasmin.
1987,
Pubmed
,
Xenbase
Cordingley,
Sequence-specific interaction of Tat protein and Tat peptides with the transactivation-responsive sequence element of human immunodeficiency virus type 1 in vitro.
1990,
Pubmed
Dabauvalle,
Karyophilic proteins: polypeptides synthesized in vitro accumulate in the nucleus on microinjection into the cytoplasm of amphibian oocytes.
1982,
Pubmed
,
Xenbase
Dang,
Nuclear and nucleolar targeting sequences of c-erb-A, c-myb, N-myc, p53, HSP70, and HIV tat proteins.
1989,
Pubmed
De Robertis,
Intracellular migration of nuclear proteins in Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Dingwall,
Protein import into the cell nucleus.
1986,
Pubmed
Dingwall,
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
1988,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Gall,
The sphere organelle contains small nuclear ribonucleoproteins.
1989,
Pubmed
,
Xenbase
Gall,
Histone genes are located at the sphere loci of newt lampbrush chromosomes.
1981,
Pubmed
Hausen,
Urea reactivates antigens in paraffin sections for immunofluorescent staining.
1982,
Pubmed
,
Xenbase
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Melton,
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
1984,
Pubmed
Milarski,
Mutational analysis of the human HSP70 protein: distinct domains for nucleolar localization and adenosine triphosphate binding.
1989,
Pubmed
Munro,
Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70.
1984,
Pubmed
Nosaka,
Nucleolar targeting signal of human T-cell leukemia virus type I rex-encoded protein is essential for cytoplasmic accumulation of unspliced viral mRNA.
1989,
Pubmed
Paine,
Nuclear envelope permeability.
1975,
Pubmed
Paine,
Nucleocytoplasmic exchange of macromolecules.
1972,
Pubmed
Robbins,
Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence.
1991,
Pubmed
,
Xenbase
Roth,
A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle.
1990,
Pubmed
,
Xenbase
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Schmidt-Zachmann,
A constitutive nucleolar protein identified as a member of the nucleoplasmin family.
1987,
Pubmed
,
Xenbase
Short,
Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties.
1988,
Pubmed
Silver,
Nuclear protein transport.
1989,
Pubmed
Siomi,
Sequence requirements for nucleolar localization of human T cell leukemia virus type I pX protein, which regulates viral RNA processing.
1988,
Pubmed
Wallace,
Protein incorporation by isolated amphibian oocytes. 3. Optimum incubation conditions.
1973,
Pubmed
,
Xenbase
Watson,
Compilation of published signal sequences.
1984,
Pubmed
Weeks,
Fragments of the HIV-1 Tat protein specifically bind TAR RNA.
1990,
Pubmed
Welch,
Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.
1984,
Pubmed
Wu,
Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes.
1991,
Pubmed
,
Xenbase
