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Proc Natl Acad Sci U S A
2001 Feb 13;984:1555-9. doi: 10.1073/pnas.98.4.1555.
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Electrophysiological characterization of specific interactions between bacterial sensory rhodopsins and their transducers.
Schmies G
,
Engelhard M
,
Wood PG
,
Nagel G
,
Bamberg E
.
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The halobacterial phototaxis receptors sensory rhodopsin I and II (SRI, SRII) enable the bacteria to seek optimal light conditions for ion pumping by bacteriorhodopsin and/or halorhodopsin. The incoming signal is transferred across the plasma membrane by means of receptor-specific transducer proteins that bind tightly to their corresponding photoreceptors. To investigate the receptor/transducer interaction, advantage is taken of the observation that both SRI and SRII can function as proton pumps. SRI from Halobacterium salinarum, which triggers the positive phototaxis, the photophobic receptor SRII from Natronobacterium pharaonis (pSRII), as well as the mutant pSRII-F86D were expressed in Xenopus oocytes. Voltage-clamp studies confirm that SRI and pSRII function as light-driven, outwardly directed proton pumps with a much stronger voltage dependence than the ion pumps bacteriorhodopsin and halorhodopsin. Coexpression of SRI and pSRII-F86D with their corresponding transducers suppresses the proton transport, revealing a tight binding and specific interaction of the two proteins. These latter results may be exploited to further analyze the binding interaction of the photoreceptors with their downstream effectors.
Bogomolni,
The photochemical reactions of bacterial sensory rhodopsin-I. Flash photolysis study in the one microsecond to eight second time window.
1987, Pubmed
Bogomolni,
The photochemical reactions of bacterial sensory rhodopsin-I. Flash photolysis study in the one microsecond to eight second time window.
1987,
Pubmed
Bogomolni,
Removal of transducer HtrI allows electrogenic proton translocation by sensory rhodopsin I.
1994,
Pubmed
Chizhov,
The photophobic receptor from Natronobacterium pharaonis: temperature and pH dependencies of the photocycle of sensory rhodopsin II.
1998,
Pubmed
Dancsházy,
Kinetics of the blue light-induced inhibition of photoelectric activity of bacteriorhodopsin.
1978,
Pubmed
Gloor,
A vector for the synthesis of cRNAs encoding Myc epitope-tagged proteins in Xenopus laevis oocytes.
1995,
Pubmed
,
Xenbase
Grygorczyk,
Measurement of erythroid band 3 protein-mediated anion transport in mRNA-injected oocytes of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Haupts,
Different modes of proton translocation by sensory rhodopsin I.
1996,
Pubmed
Haupts,
The photoreceptor sensory rhodopsin I as a two-photon-driven proton pump.
1995,
Pubmed
Jung,
Suppressor mutation analysis of the sensory rhodopsin I-transducer complex: insights into the color-sensing mechanism.
1998,
Pubmed
Michel,
Electrochemical proton gradient across the cell membrane of Halobacterium halobium: comparison of the light-induced increase with the increase of intracellular adenosine triphosphate under steady-state illumination.
1980,
Pubmed
Nagel,
Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H+ pumping.
1995,
Pubmed
,
Xenbase
Nagel,
Voltage dependence of proton pumping by bacteriorhodopsin is regulated by the voltage-sensitive ratio of M1 to M2.
1998,
Pubmed
,
Xenbase
Olson,
Removal of the transducer protein from sensory rhodopsin I exposes sites of proton release and uptake during the receptor photocycle.
1993,
Pubmed
Rath,
The Schiff base counterion of bacteriorhodopsin is protonated in sensory rhodopsin I: spectroscopic and functional characterization of the mutated proteins D76N and D76A.
1994,
Pubmed
Sasaki,
Proton circulation during the photocycle of sensory rhodopsin II.
1999,
Pubmed
Schmies,
Sensory rhodopsin II from the haloalkaliphilic natronobacterium pharaonis: light-activated proton transfer reactions.
2000,
Pubmed
Schmies,
Functional expression of His-tagged sensory rhodopsin I in Escherichia coli.
2000,
Pubmed
Spudich,
Variations on a molecular switch: transport and sensory signalling by archaeal rhodopsins.
1998,
Pubmed
Subramaniam,
Replacement of leucine-93 by alanine or threonine slows down the decay of the N and O intermediates in the photocycle of bacteriorhodopsin: implications for proton uptake and 13-cis-retinal----all-trans-retinal reisomerization.
1991,
Pubmed
Tittor,
Inversion of proton translocation in bacteriorhodopsin mutants D85N, D85T, and D85,96N.
1994,
Pubmed
Wegener,
Time-resolved detection of transient movement of helix F in spin-labelled pharaonis sensory rhodopsin II.
2000,
Pubmed
Zhang,
The specificity of interaction of archaeal transducers with their cognate sensory rhodopsins is determined by their transmembrane helices.
1999,
Pubmed
Zhang,
HtrI is a dimer whose interface is sensitive to receptor photoactivation and His-166 replacements in sensory rhodopsin I.
1998,
Pubmed
