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The interactions with Ro60 and La differentially affect nuclear export of hY1 RNA.
Simons FH
,
Rutjes SA
,
van Venrooij WJ
,
Pruijn GJ
.
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Ro RNPs are evolutionarily conserved ribonucleoprotein particles that consist of a small RNA, known as Y RNA, associated with several proteins, such as La, Ro60, and Ro52. The Y RNAs (Y1-Y5), which are transcribed by RNA polymerase III, have been shown to reside almost exclusively in the cytoplasm as Ro RNPs. To obtain more insight into the nuclear export pathway of Y RNAs, hY1 RNA export was studied in Xenopus laevis oocytes. Injection of various hY1 RNA mutants showed that an intact Ro60 binding site is a prerequisite for nuclear export, whereas the presence of an intact La binding site resulted in strong nuclear retention of hY1 RNA. Competition studies with various classes of RNAs indicated that, in addition to Ro60, another titratable factor was necessary for nuclear export of hY1 RNA. This factor appears also to be involved in nuclear export of tRNA. Because export of hY1 RNA could not be blocked by a synthetic peptide containing the recently identified nuclear export signal of the HIV-1 Rev protein, nuclear export of hY1 RNA does not seem to be dependent on a Rev-like nuclear export signal.
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995, Pubmed,
Xenbase
Boelens,
Nuclear retention of RNA as a mechanism for localization.
1995,
Pubmed
,
Xenbase
Boire,
Human Ro ribonucleoprotein particles: characterization of native structure and stable association with the La polypeptide.
1990,
Pubmed
Chow,
Delineation of structural domains in eukaryotic 5S rRNA with a rhodium probe.
1992,
Pubmed
,
Xenbase
Eckner,
Mature mRNA 3' end formation stimulates RNA export from the nucleus.
1991,
Pubmed
Farris,
Y3 is the most conserved small RNA component of Ro ribonucleoprotein complexes in vertebrate species.
1995,
Pubmed
,
Xenbase
Fischer,
The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs.
1995,
Pubmed
Fischer,
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
1994,
Pubmed
,
Xenbase
Gerace,
Nuclear export signals and the fast track to the cytoplasm.
1995,
Pubmed
Guddat,
Protein-mediated nuclear export of RNA: 5S rRNA containing small RNPs in xenopus oocytes.
1990,
Pubmed
,
Xenbase
Hamm,
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Hamm,
In vitro assembly of U1 snRNPs.
1987,
Pubmed
,
Xenbase
Hamm,
Monomethylated cap structures facilitate RNA export from the nucleus.
1990,
Pubmed
,
Xenbase
He,
Nuclear export of signal recognition particle RNA is a facilitated process that involves the Alu sequence domain.
1994,
Pubmed
,
Xenbase
Hendrick,
Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleoproteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells.
1981,
Pubmed
Izaurralde,
A nuclear cap binding protein complex involved in pre-mRNA splicing.
1994,
Pubmed
,
Xenbase
Izaurralde,
RNA export.
1995,
Pubmed
Jarmolowski,
Nuclear export of different classes of RNA is mediated by specific factors.
1994,
Pubmed
,
Xenbase
Mathews,
La antigen recognizes and binds to the 3'-oligouridylate tail of a small RNA.
1984,
Pubmed
O'Brien,
Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins.
1993,
Pubmed
,
Xenbase
Peek,
Subcellular distribution of Ro ribonucleoprotein complexes and their constituents.
1993,
Pubmed
Piñol-Roma,
hnRNP proteins: localization and transport between the nucleus and the cytoplasm.
1993,
Pubmed
Piñol-Roma,
Transcription-dependent and transcription-independent nuclear transport of hnRNP proteins.
1991,
Pubmed
Piñol-Roma,
Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm.
1992,
Pubmed
,
Xenbase
Pruijn,
Analysis of protein--RNA interactions within Ro ribonucleoprotein complexes.
1991,
Pubmed
Pruijn,
Anti-La monoclonal antibodies recognizing epitopes within the RNA-binding domain of the La protein show differential capacities to immunoprecipitate RNA-associated La protein.
1995,
Pubmed
,
Xenbase
Pruijn,
Ro RNP associated Y RNAs are highly conserved among mammals.
1993,
Pubmed
Simons,
Analysis of the intracellular localization and assembly of Ro ribonucleoprotein particles by microinjection into Xenopus laevis oocytes.
1994,
Pubmed
,
Xenbase
Slobbe,
Ro ribonucleoprotein assembly in vitro. Identification of RNA-protein and protein-protein interactions.
1992,
Pubmed
Stefano,
Purified lupus antigen La recognizes an oligouridylate stretch common to the 3' termini of RNA polymerase III transcripts.
1984,
Pubmed
Tobian,
tRNA nuclear transport: defining the critical regions of human tRNAimet by point mutagenesis.
1985,
Pubmed
,
Xenbase
Van Horn,
Caenorhabditis elegans embryos contain only one major species of Ro RNP.
1995,
Pubmed
,
Xenbase
van Venrooij,
Structure and function of La and Ro RNPs.
1993,
Pubmed
Veldhoven,
Characterization of murine monoclonal antibodies against 60-kD Ro/SS-A and La/SS-B autoantigens.
1995,
Pubmed
Wolin,
Genes for two small cytoplasmic Ro RNAs are adjacent and appear to be single-copy in the human genome.
1983,
Pubmed
Wolin,
The Ro small cytoplasmic ribonucleoproteins: identification of the antigenic protein and its binding site on the Ro RNAs.
1984,
Pubmed
Zasloff,
tRNA transport from the nucleus in a eukaryotic cell: carrier-mediated translocation process.
1983,
Pubmed
,
Xenbase
