Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Molecular determinants of inactivation within the I-II linker of alpha1E (CaV2.3) calcium channels.
Berrou L
,
Bernatchez G
,
Parent L
.
???displayArticle.abstract???
Voltage-dependent inactivation of CaV2.3 channels was investigated using point mutations in the beta-subunit-binding site (AID) of the I-II linker. The quintuple mutant alpha1E N381K + R384L + A385D + D388T + K389Q (NRADK-KLDTQ) inactivated like the wild-type alpha1E. In contrast, mutations of alpha1E at position R378 (position 5 of AID) into negatively charged residues Glu (E) or Asp (D) significantly slowed inactivation kinetics and shifted the voltage dependence of inactivation to more positive voltages. When co-injected with beta3, R378E inactivated with tau(inact) = 538 +/- 54 ms (n = 14) as compared with 74 +/- 4 ms (n = 21) for alpha1E (p < 0.001) with a mid-potential of inactivation E(0.5) = -44 +/- 2 mV (n = 10) for R378E as compared with E(0.5) = -64 +/- 3 mV (n = 9) for alpha1E. A series of mutations at position R378 suggest that positively charged residues could promote voltage-dependent inactivation. R378K behaved like the wild-type alpha1E whereas R378Q displayed intermediate inactivation kinetics. The reverse mutation E462R in the L-type alpha1C (CaV1.2) produced channels with inactivation properties comparable to alpha1E R378E. Hence, position 5 of the AID motif in the I-II linker could play a significant role in the inactivation of Ca(V)1.2 and CaV2.3 channels.
Adams,
Structural regions of the cardiac Ca channel alpha subunit involved in Ca-dependent inactivation.
1997, Pubmed
Adams,
Structural regions of the cardiac Ca channel alpha subunit involved in Ca-dependent inactivation.
1997,
Pubmed
Armstrong,
Inactivation of the sodium channel. II. Gating current experiments.
1977,
Pubmed
Bean,
Sodium channel inactivation in the crayfish giant axon. Must channels open before inactivating?
1981,
Pubmed
Bernatchez,
Mutations in the EF-hand motif impair the inactivation of barium currents of the cardiac alpha1C channel.
1998,
Pubmed
,
Xenbase
Bichet,
The I-II loop of the Ca2+ channel alpha1 subunit contains an endoplasmic reticulum retention signal antagonized by the beta subunit.
2000,
Pubmed
,
Xenbase
Bourinet,
Splicing of alpha 1A subunit gene generates phenotypic variants of P- and Q-type calcium channels.
1999,
Pubmed
,
Xenbase
Bourinet,
Determinants of the G protein-dependent opioid modulation of neuronal calcium channels.
1996,
Pubmed
,
Xenbase
Campbell,
Inhibition of the interaction of G protein G(o) with calcium channels by the calcium channel beta-subunit in rat neurones.
1995,
Pubmed
Castellano,
Cloning and expression of a third calcium channel beta subunit.
1993,
Pubmed
,
Xenbase
Cens,
Voltage and calcium use the same molecular determinants to inactivate calcium channels.
1999,
Pubmed
Cribbs,
Molecular cloning and functional expression of Ca(v)3.1c, a T-type calcium channel from human brain.
2000,
Pubmed
Cribbs,
Cloning and characterization of alpha1H from human heart, a member of the T-type Ca2+ channel gene family.
1998,
Pubmed
de Leon,
Essential Ca(2+)-binding motif for Ca(2+)-sensitive inactivation of L-type Ca2+ channels.
1995,
Pubmed
Demo,
The inactivation gate of the Shaker K+ channel behaves like an open-channel blocker.
1991,
Pubmed
Deng,
Site-directed mutagenesis of virtually any plasmid by eliminating a unique site.
1992,
Pubmed
De Waard,
Identification of critical amino acids involved in alpha1-beta interaction in voltage-dependent Ca2+ channels.
1996,
Pubmed
,
Xenbase
De Waard,
Direct binding of G-protein betagamma complex to voltage-dependent calcium channels.
1997,
Pubmed
,
Xenbase
De Waard,
Subunit regulation of the neuronal alpha 1A Ca2+ channel expressed in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Dolphin,
Mechanisms of modulation of voltage-dependent calcium channels by G proteins.
1998,
Pubmed
Ellinor,
Functional expression of a rapidly inactivating neuronal calcium channel.
1993,
Pubmed
,
Xenbase
Ertel,
Nomenclature of voltage-gated calcium channels.
2000,
Pubmed
Gerster,
Current modulation and membrane targeting of the calcium channel alpha1C subunit are independent functions of the beta subunit.
1999,
Pubmed
Herlitze,
Molecular determinants of inactivation and G protein modulation in the intracellular loop connecting domains I and II of the calcium channel alpha1A subunit.
1997,
Pubmed
Hoshi,
Two types of inactivation in Shaker K+ channels: effects of alterations in the carboxy-terminal region.
1991,
Pubmed
,
Xenbase
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Kellenberger,
Molecular analysis of the putative inactivation particle in the inactivation gate of brain type IIA Na+ channels.
1997,
Pubmed
Kuo,
Na+ channels must deactivate to recover from inactivation.
1994,
Pubmed
Lacerda,
T-type and N-type calcium channels of Xenopus oocytes: evidence for specific interactions with beta subunits.
1994,
Pubmed
,
Xenbase
Lee,
Ca2+/calmodulin binds to and modulates P/Q-type calcium channels.
1999,
Pubmed
Lee,
Cloning and expression of a novel member of the low voltage-activated T-type calcium channel family.
1999,
Pubmed
,
Xenbase
Li,
Functional modulation of brain sodium channels by cAMP-dependent phosphorylation.
1992,
Pubmed
Mehrke,
Receptor-mediated modulation of recombinant neuronal class E calcium channels.
1997,
Pubmed
Monteil,
Molecular and functional properties of the human alpha(1G) subunit that forms T-type calcium channels.
2000,
Pubmed
Page,
Identification of the amino terminus of neuronal Ca2+ channel alpha1 subunits alpha1B and alpha1E as an essential determinant of G-protein modulation.
1998,
Pubmed
,
Xenbase
Page,
The intracellular loop between domains I and II of the B-type calcium channel confers aspects of G-protein sensitivity to the E-type calcium channel.
1997,
Pubmed
Parent,
Voltage-dependent inactivation in a cardiac-skeletal chimeric calcium channel.
1995,
Pubmed
,
Xenbase
Parent,
Glutamate substitution in repeat IV alters divalent and monovalent cation permeation in the heart Ca2+ channel.
1995,
Pubmed
,
Xenbase
Parent,
Subunit regulation of the human brain alpha 1E calcium channel.
1997,
Pubmed
,
Xenbase
Patil,
Preferential closed-state inactivation of neuronal calcium channels.
1998,
Pubmed
Patton,
Amino acid residues required for fast Na(+)-channel inactivation: charge neutralizations and deletions in the III-IV linker.
1992,
Pubmed
Perez-Reyes,
Molecular characterization of a neuronal low-voltage-activated T-type calcium channel.
1998,
Pubmed
,
Xenbase
Peterson,
Critical determinants of Ca(2+)-dependent inactivation within an EF-hand motif of L-type Ca(2+) channels.
2000,
Pubmed
Peterson,
Calmodulin is the Ca2+ sensor for Ca2+ -dependent inactivation of L-type calcium channels.
1999,
Pubmed
Piedras-Rentería,
Antisense oligonucleotides against alpha1E reduce R-type calcium currents in cerebellar granule cells.
1998,
Pubmed
Pragnell,
Calcium channel beta-subunit binds to a conserved motif in the I-II cytoplasmic linker of the alpha 1-subunit.
1994,
Pubmed
Qin,
Ca2+-induced inhibition of the cardiac Ca2+ channel depends on calmodulin.
1999,
Pubmed
,
Xenbase
Randall,
Contrasting biophysical and pharmacological properties of T-type and R-type calcium channels.
1997,
Pubmed
Saegusa,
Altered pain responses in mice lacking alpha 1E subunit of the voltage-dependent Ca2+ channel.
2000,
Pubmed
Sokolov,
Inactivation determinant in the I-II loop of the Ca2+ channel alpha1-subunit and beta-subunit interaction affect sensitivity for the phenylalkylamine (-)gallopamil.
1999,
Pubmed
,
Xenbase
Stephens,
The alpha1B Ca2+ channel amino terminus contributes determinants for beta subunit-mediated voltage-dependent inactivation properties.
2000,
Pubmed
Stotz,
Fast inactivation of voltage-dependent calcium channels. A hinged-lid mechanism?
2000,
Pubmed
Tareilus,
A Xenopus oocyte beta subunit: evidence for a role in the assembly/expression of voltage-gated calcium channels that is separate from its role as a regulatory subunit.
1997,
Pubmed
,
Xenbase
Toth,
Selective G-protein regulation of neuronal calcium channels.
1996,
Pubmed
Walker,
A new beta subtype-specific interaction in alpha1A subunit controls P/Q-type Ca2+ channel activation.
1999,
Pubmed
,
Xenbase
Walker,
Subunit interaction sites in voltage-dependent Ca2+ channels: role in channel function.
1998,
Pubmed
West,
A cluster of hydrophobic amino acid residues required for fast Na(+)-channel inactivation.
1992,
Pubmed
,
Xenbase
Williams,
Structure and functional expression of alpha 1, alpha 2, and beta subunits of a novel human neuronal calcium channel subtype.
1992,
Pubmed
,
Xenbase
Yamaguchi,
Multiple modulation pathways of calcium channel activity by a beta subunit. Direct evidence of beta subunit participation in membrane trafficking of the alpha1C subunit.
1998,
Pubmed
,
Xenbase
Yamakura,
Amino acid volume and hydropathy of a transmembrane site determine glycine and anesthetic sensitivity of glycine receptors.
1999,
Pubmed
,
Xenbase
Zamponi,
Crosstalk between G proteins and protein kinase C mediated by the calcium channel alpha1 subunit.
1997,
Pubmed
,
Xenbase
Zhang,
Multiple structural elements in voltage-dependent Ca2+ channels support their inhibition by G proteins.
1996,
Pubmed
,
Xenbase
Zhou,
Feedback inhibition of Ca2+ channels by Ca2+ depends on a short sequence of the C terminus that does not include the Ca2+ -binding function of a motif with similarity to Ca2+ -binding domains.
1997,
Pubmed
,
Xenbase
Zühlke,
Calmodulin supports both inactivation and facilitation of L-type calcium channels.
1999,
Pubmed
,
Xenbase
