Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Endocrinology
2016 Jan 01;1571:405-16. doi: 10.1210/en.2015-1135.
Show Gene links
Show Anatomy links
Embryonic Poly(A)-Binding Protein (EPAB) Is Required for Granulosa Cell EGF Signaling and Cumulus Expansion in Female Mice.
Yang CR
,
Lowther KM
,
Lalioti MD
,
Seli E
.
???displayArticle.abstract???
Embryonic poly(A)-binding protein (EPAB) is the predominant poly(A)-binding protein in Xenopus, mouse, and human oocytes and early embryos before zygotic genome activation. EPAB is required for translational activation of maternally stored mRNAs in the oocyte and Epab(-/-) female mice are infertile due to impaired oocyte maturation, cumulus expansion, and ovulation. The aim of this study was to characterize the mechanism of follicular somatic cell dysfunction in Epab(-/-) mice. Using a coculture system of oocytectomized cumulus oophorus complexes (OOXs) with denuded oocytes, we found that when wild-type OOXs were cocultured with Epab(-/-) oocytes, or when Epab(-/-) OOXs were cocultured with WT oocytes, cumulus expansion failed to occur in response to epidermal growth factor (EGF). This finding suggests that oocytes and cumulus cells (CCs) from Epab(-/-) mice fail to send and receive the necessary signals required for cumulus expansion. The abnormalities in Epab(-/-) CCs are not due to lower expression of the oocyte-derived factors growth differentiation factor 9 or bone morphogenetic protein 15, because Epab(-/-) oocytes express these proteins at comparable levels with WT. Epab(-/-) granulosa cells (GCs) exhibit decreased levels of phosphorylated MEK1/2, ERK1/2, and p90 ribosomal S6 kinase in response to lutenizing hormone and EGF treatment, as well as decreased phosphorylation of the EGF receptor. In conclusion, EPAB, which is oocyte specific, is required for the ability of CCs and GCs to become responsive to LH and EGF signaling. These results emphasize the importance of oocyte-somatic communication for GC and CC function.
Adashi,
Endocrinology of the ovary.
1994,
Pubmed
Amsterdam,
Distribution of binding sites for human chorionic gonadotropin in the preovulatory follicle of the rat.
1975,
Pubmed
Ashkenazi,
Epidermal growth factor family members: endogenous mediators of the ovulatory response.
2005,
Pubmed
Barnard,
Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation.
2004,
Pubmed
,
Xenbase
Blondin,
Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes.
1995,
Pubmed
Buccione,
FSH-induced expansion of the mouse cumulus oophorus in vitro is dependent upon a specific factor(s) secreted by the oocyte.
1990,
Pubmed
Carlone,
Functional interactions, phosphorylation, and levels of 3',5'-cyclic adenosine monophosphate-regulatory element binding protein and steroidogenic factor-1 mediate hormone-regulated and constitutive expression of aromatase in gonadal cells.
1997,
Pubmed
Chang,
Genetic analysis of the mammalian transforming growth factor-beta superfamily.
2002,
Pubmed
Chen,
Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition.
2011,
Pubmed
,
Xenbase
Chen,
Functional significance of cumulus expansion in the mouse: roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass.
1993,
Pubmed
Collier,
The DAZL family proteins are PABP-binding proteins that regulate translation in germ cells.
2005,
Pubmed
,
Xenbase
Conti,
Role of the epidermal growth factor network in ovarian follicles.
2006,
Pubmed
Davis,
Anovulation in cyclooxygenase-2-deficient mice is restored by prostaglandin E2 and interleukin-1beta.
1999,
Pubmed
Diaz,
The preantral granulosa cell to cumulus cell transition in the mouse ovary: development of competence to undergo expansion.
2006,
Pubmed
Diaz,
Oocytes determine cumulus cell lineage in mouse ovarian follicles.
2007,
Pubmed
Dong,
Growth differentiation factor-9 is required during early ovarian folliculogenesis.
1996,
Pubmed
Downs,
Specificity of epidermal growth factor action on maturation of the murine oocyte and cumulus oophorus in vitro.
1989,
Pubmed
Downs,
EGF-like peptides mediate FSH-induced maturation of cumulus cell-enclosed mouse oocytes.
2008,
Pubmed
Dube,
The bone morphogenetic protein 15 gene is X-linked and expressed in oocytes.
1998,
Pubmed
Elvin,
Paracrine actions of growth differentiation factor-9 in the mammalian ovary.
1999,
Pubmed
Eppig,
Intercommunication between mammalian oocytes and companion somatic cells.
1991,
Pubmed
Eppig,
Murine oocytes suppress expression of luteinizing hormone receptor messenger ribonucleic acid by granulosa cells.
1997,
Pubmed
Eppig,
Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes.
1994,
Pubmed
Eppig,
Oocyte control of ovarian follicular development and function in mammals.
2001,
Pubmed
Fan,
MAPK3/1 (ERK1/2) in ovarian granulosa cells are essential for female fertility.
2009,
Pubmed
Freimann,
EGF-like factor epiregulin and amphiregulin expression is regulated by gonadotropins/cAMP in human ovarian follicular cells.
2004,
Pubmed
Friend,
Embryonic poly(A)-binding protein (ePAB) phosphorylation is required for Xenopus oocyte maturation.
2012,
Pubmed
,
Xenbase
Fülöp,
Impaired cumulus mucification and female sterility in tumor necrosis factor-induced protein-6 deficient mice.
2003,
Pubmed
Gebauer,
Synthesis and function of Mos: the control switch of vertebrate oocyte meiosis.
1997,
Pubmed
,
Xenbase
Gilchrist,
Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality.
2008,
Pubmed
Groisman,
CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division.
2000,
Pubmed
,
Xenbase
Guéripel,
Oocyte bone morphogenetic protein 15, but not growth differentiation factor 9, is increased during gonadotropin-induced follicular development in the immature mouse and is associated with cumulus oophorus expansion.
2006,
Pubmed
Guzeloglu-Kayisli,
Embryonic poly(A)-binding protein (EPAB) is required for oocyte maturation and female fertility in mice.
2012,
Pubmed
,
Xenbase
Guzeloglu-Kayisli,
Identification and characterization of human embryonic poly(A) binding protein (EPAB).
2008,
Pubmed
,
Xenbase
Guzeloglu-Kayisli,
Human embryonic poly(A)-binding protein (EPAB) alternative splicing is differentially regulated in human oocytes and embryos.
2014,
Pubmed
,
Xenbase
Hake,
Translational regulation of maternal mRNA.
1997,
Pubmed
,
Xenbase
Hess,
Inter-alpha-inhibitor binding to hyaluronan in the cumulus extracellular matrix is required for optimal ovulation and development of mouse oocytes.
1999,
Pubmed
Hsieh,
Genetic dissection of epidermal growth factor receptor signaling during luteinizing hormone-induced oocyte maturation.
2011,
Pubmed
Hsieh,
Epidermal growth factor-like growth factors in the follicular fluid: role in oocyte development and maturation.
2009,
Pubmed
Hsieh,
Luteinizing hormone-dependent activation of the epidermal growth factor network is essential for ovulation.
2007,
Pubmed
Huang,
The human oocyte and cumulus cells relationship: new insights from the cumulus cell transcriptome.
2010,
Pubmed
Hunzicker-Dunn,
FSH signaling pathways in immature granulosa cells that regulate target gene expression: branching out from protein kinase A.
2006,
Pubmed
Hussein,
Oocyte-secreted factors enhance oocyte developmental competence.
2006,
Pubmed
Kawashima,
EGF-like factors induce expansion of the cumulus cell-oocyte complexes by activating calpain-mediated cell movement.
2012,
Pubmed
Kim,
RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB.
2007,
Pubmed
,
Xenbase
Lowther,
Embryonic Poly(A)-Binding Protein Is Required During Early Stages of Mouse Oocyte Development for Chromatin Organization, Transcriptional Silencing, and Meiotic Competence.
2015,
Pubmed
,
Xenbase
Luetteke,
Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development.
1999,
Pubmed
Matova,
Comparative aspects of animal oogenesis.
2001,
Pubmed
,
Xenbase
McGrath,
Oocyte-specific expression of growth/differentiation factor-9.
1995,
Pubmed
McPherron,
GDF-3 and GDF-9: two new members of the transforming growth factor-beta superfamily containing a novel pattern of cysteines.
1993,
Pubmed
,
Xenbase
Mehlmann,
Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation.
2005,
Pubmed
Mendez,
Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.
2000,
Pubmed
,
Xenbase
Ochsner,
Disrupted function of tumor necrosis factor-alpha-stimulated gene 6 blocks cumulus cell-oocyte complex expansion.
2003,
Pubmed
Oh,
Timely translation during the mouse oocyte-to-embryo transition.
2000,
Pubmed
,
Xenbase
Otsuka,
Bone morphogenetic protein-15. Identification of target cells and biological functions.
2000,
Pubmed
Otsuka,
Bone morphogenetic protein-15 inhibits follicle-stimulating hormone (FSH) action by suppressing FSH receptor expression.
2001,
Pubmed
Padmanabhan,
Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation.
2006,
Pubmed
,
Xenbase
Panigone,
Luteinizing hormone signaling in preovulatory follicles involves early activation of the epidermal growth factor receptor pathway.
2008,
Pubmed
Park,
EGF-like growth factors as mediators of LH action in the ovulatory follicle.
2004,
Pubmed
Peng,
Localization of luteinizing hormone receptor messenger ribonucleic acid expression in ovarian cell types during follicle development and ovulation.
1991,
Pubmed
Piqué,
A combinatorial code for CPE-mediated translational control.
2008,
Pubmed
,
Xenbase
Radford,
Translational control by cytoplasmic polyadenylation in Xenopus oocytes.
2008,
Pubmed
,
Xenbase
Reizel,
Sustained activity of the EGF receptor is an absolute requisite for LH-induced oocyte maturation and cumulus expansion.
2010,
Pubmed
Richards,
Ovulation: new dimensions and new regulators of the inflammatory-like response.
2002,
Pubmed
Sayasith,
Human chorionic gonadotropin-dependent up-regulation of epiregulin and amphiregulin in equine and bovine follicles during the ovulatory process.
2013,
Pubmed
Schneider,
NIH Image to ImageJ: 25 years of image analysis.
2012,
Pubmed
Seger,
The ERK signaling cascade inhibits gonadotropin-stimulated steroidogenesis.
2001,
Pubmed
Sekiguchi,
Expression of epiregulin and amphiregulin in the rat ovary.
2004,
Pubmed
Seli,
An embryonic poly(A)-binding protein (ePAB) is expressed in mouse oocytes and early preimplantation embryos.
2005,
Pubmed
,
Xenbase
Stutz,
Masking, unmasking, and regulated polyadenylation cooperate in the translational control of a dormant mRNA in mouse oocytes.
1998,
Pubmed
Su,
Synergistic roles of BMP15 and GDF9 in the development and function of the oocyte-cumulus cell complex in mice: genetic evidence for an oocyte-granulosa cell regulatory loop.
2004,
Pubmed
Su,
Mouse oocyte control of granulosa cell development and function: paracrine regulation of cumulus cell metabolism.
2009,
Pubmed
Sugiura,
Estrogen promotes the development of mouse cumulus cells in coordination with oocyte-derived GDF9 and BMP15.
2010,
Pubmed
Vanderhyden,
Developmental pattern of the secretion of cumulus expansion-enabling factor by mouse oocytes and the role of oocytes in promoting granulosa cell differentiation.
1990,
Pubmed
Vasudevan,
Metazoan oocyte and early embryo development program: a progression through translation regulatory cascades.
2006,
Pubmed
,
Xenbase
Vitt,
Growth differentiation factor-9 stimulates proliferation but suppresses the follicle-stimulating hormone-induced differentiation of cultured granulosa cells from small antral and preovulatory rat follicles.
2000,
Pubmed
Voeltz,
A novel embryonic poly(A) binding protein, ePAB, regulates mRNA deadenylation in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Wayne,
Follicle-stimulating hormone induces multiple signaling cascades: evidence that activation of Rous sarcoma oncogene, RAS, and the epidermal growth factor receptor are critical for granulosa cell differentiation.
2007,
Pubmed
Wigglesworth,
Bidirectional communication between oocytes and ovarian follicular somatic cells is required for meiotic arrest of mammalian oocytes.
2013,
Pubmed
Wilkie,
Embryonic poly(A)-binding protein stimulates translation in germ cells.
2005,
Pubmed
,
Xenbase
Yan,
Synergistic roles of bone morphogenetic protein 15 and growth differentiation factor 9 in ovarian function.
2001,
Pubmed
Yoshino,
A unique preovulatory expression pattern plays a key role in the physiological functions of BMP-15 in the mouse.
2006,
Pubmed
