Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Different types and patterns of spontaneous electrical activity drive many aspects of neuronal differentiation. Neurons in the developing Xenopus spinal cord exhibit calcium spikes, which regulate gene transcription and neurotransmitter specification. The ionic currents necessary for spike production have been described. However, the mechanisms that generate the onset of this activity and the basis of its regulation remain unclear. Although signaling molecules appear to act on plasma membrane receptors to trigger calcium spike activity, other mechanisms for spontaneous calcium spike regulation may exist as well. Here, we analyze the developmental expression of the Na(+), K(+)-ATPase beta3 subunit in Xenopus tropicalis embryos and show that its levels are downregulated at a time during embryonic development that coincides with the onset of prominent calcium spike activity in spinal neurons. Inhibition of an earlier increase in beta3 expression leads to more depolarized resting membrane potentials and results in later reduction of spike activity. This suppression of beta3 levels also reduces expression of the store-operated calcium channel subunit, Orai1. These findings suggest that the Na(+), K(+)-ATPase plays a role in initiating calcium spike activity and regulating calcium homeostasis.
Antonicek,
Biochemical and functional characterization of a novel neuron-glia adhesion molecule that is involved in neuronal migration.
1987, Pubmed
Antonicek,
Biochemical and functional characterization of a novel neuron-glia adhesion molecule that is involved in neuronal migration.
1987,
Pubmed
Béguin,
The gamma subunit is a specific component of the Na,K-ATPase and modulates its transport function.
1997,
Pubmed
,
Xenbase
Berridge,
Neuronal calcium signaling.
1998,
Pubmed
Blackshaw,
Alterations in resting membrane properties during neural plate stages of development of the nervous system.
1976,
Pubmed
Borodinsky,
Activity-dependent homeostatic specification of transmitter expression in embryonic neurons.
2004,
Pubmed
,
Xenbase
Bouron,
Activation of a capacitative Ca(2+) entry pathway by store depletion in cultured hippocampal neurones.
2000,
Pubmed
Breckenridge,
Intracellular sodium and the differentiation of amphibian embryonic neurones.
1982,
Pubmed
,
Xenbase
Bronchain,
The olig family: phylogenetic analysis and early gene expression in Xenopus tropicalis.
2007,
Pubmed
,
Xenbase
Chemin,
Neuronal T-type alpha 1H calcium channels induce neuritogenesis and expression of high-voltage-activated calcium channels in the NG108-15 cell line.
2002,
Pubmed
Conklin,
Local calcium transients contribute to disappearance of pFAK, focal complex removal and deadhesion of neuronal growth cones and fibroblasts.
2005,
Pubmed
,
Xenbase
Davies,
Primary sequence and developmental expression pattern of mRNAs and protein for an alpha1 subunit of the sodium pump cloned from the neural plate of Xenopus laevis.
1996,
Pubmed
,
Xenbase
Desarmenien,
In vivo development of voltage-dependent ionic currents in embryonic Xenopus spinal neurons.
1993,
Pubmed
,
Xenbase
Eid,
Xenopus Na,K-ATPase: primary sequence of the beta2 subunit and in situ localization of alpha1, beta1, and gamma expression during pronephric kidney development.
2001,
Pubmed
,
Xenbase
Emptage,
Calcium stores in hippocampal synaptic boutons mediate short-term plasticity, store-operated Ca2+ entry, and spontaneous transmitter release.
2001,
Pubmed
Feske,
A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function.
2006,
Pubmed
Filippi,
The basic helix-loop-helix olig3 establishes the neural plate boundary of the trunk and is necessary for development of the dorsal spinal cord.
2005,
Pubmed
Geering,
The functional role of beta subunits in oligomeric P-type ATPases.
2001,
Pubmed
Gloor,
The adhesion molecule on glia (AMOG) is a homologue of the beta subunit of the Na,K-ATPase.
1990,
Pubmed
Gomez,
Filopodial calcium transients promote substrate-dependent growth cone turning.
2001,
Pubmed
,
Xenbase
Gomez,
In vivo regulation of axon extension and pathfinding by growth-cone calcium transients.
1999,
Pubmed
,
Xenbase
Good,
A nervous system-specific isotype of the beta subunit of Na+,K(+)-ATPase expressed during early development of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Gottmann,
Development of inward currents in chick sensory and autonomic neuronal precursor cells in culture.
1988,
Pubmed
Gu,
Distinct aspects of neuronal differentiation encoded by frequency of spontaneous Ca2+ transients.
1995,
Pubmed
,
Xenbase
Gu,
Spontaneous neuronal calcium spikes and waves during early differentiation.
1994,
Pubmed
,
Xenbase
Gu,
Low-threshold Ca2+ current and its role in spontaneous elevations of intracellular Ca2+ in developing Xenopus neurons.
1993,
Pubmed
,
Xenbase
Hayes,
Synaptic junction development in the spinal cord of an amphibian embryo: an electron microscope study.
1973,
Pubmed
,
Xenbase
Holliday,
Spontaneous calcium influx and its roles in differentiation of spinal neurons in culture.
1990,
Pubmed
,
Xenbase
Holliday,
Calcium-induced release of calcium regulates differentiation of cultured spinal neurons.
1991,
Pubmed
Ikeda,
Malfunction of respiratory-related neuronal activity in Na+, K+-ATPase alpha2 subunit-deficient mice is attributable to abnormal Cl- homeostasis in brainstem neurons.
2004,
Pubmed
Ikeda,
Degeneration of the amygdala/piriform cortex and enhanced fear/anxiety behaviors in sodium pump alpha2 subunit (Atp1a2)-deficient mice.
2003,
Pubmed
James,
Identification of a specific role for the Na,K-ATPase alpha 2 isoform as a regulator of calcium in the heart.
1999,
Pubmed
Kaplan,
Biochemistry of Na,K-ATPase.
2002,
Pubmed
Kessaris,
Ventral neurogenesis and the neuron-glial switch.
2001,
Pubmed
Koizumi,
RNA interference screen to identify genes required for Drosophila embryonic nervous system development.
2007,
Pubmed
Lavoie,
The molar ratios of alpha and beta subunits of the Na+-K+-ATPase differ in distinct subcellular membranes from rat skeletal muscle.
1997,
Pubmed
Lescale-Matys,
Na(+)-K(+)-ATPase alpha 1- and beta 1-subunit degradation: evidence for multiple subunit specific rates.
1993,
Pubmed
Lewis,
The molecular choreography of a store-operated calcium channel.
2007,
Pubmed
Lockery,
Reconstruction of action potential development from whole-cell currents of differentiating spinal neurons.
1992,
Pubmed
Louvi,
Notch signalling in vertebrate neural development.
2006,
Pubmed
Lu,
Common developmental requirement for Olig function indicates a motor neuron/oligodendrocyte connection.
2002,
Pubmed
Madan,
Na/K-ATPase beta1 subunit expression is required for blastocyst formation and normal assembly of trophectoderm tight junction-associated proteins.
2007,
Pubmed
Magyar,
Degeneration of neural cells in the central nervous system of mice deficient in the gene for the adhesion molecule on Glia, the beta 2 subunit of murine Na,K-ATPase.
1994,
Pubmed
McCobb,
Development alters the expression of calcium currents in chick limb motoneurons.
1989,
Pubmed
McEwen,
Heterophilic interactions of sodium channel beta1 subunits with axonal and glial cell adhesion molecules.
2004,
Pubmed
Messenger,
The function of the sodium pump during differentiation of amphibian embryonic neurones.
1979,
Pubmed
,
Xenbase
Messenger,
Primary neuronal differentiation in Xenopus embryos is linked to the beta(3) subunit of the sodium pump.
2000,
Pubmed
,
Xenbase
Mircheff,
Synthesis and translocation of Na(+)-K(+)-ATPase alpha- and beta-subunits to plasma membrane in MDCK cells.
1992,
Pubmed
Moseley,
The Na,K-ATPase alpha 2 isoform is expressed in neurons, and its absence disrupts neuronal activity in newborn mice.
2003,
Pubmed
Müller-Husmann,
Functional characterization of beta isoforms of murine Na,K-ATPase. The adhesion molecule on glia (AMOG/beta 2), but not beta 1, promotes neurite outgrowth.
1993,
Pubmed
O'Dowd,
Development of voltage-dependent calcium, sodium, and potassium currents in Xenopus spinal neurons.
1988,
Pubmed
,
Xenbase
Orlowski,
Tissue-specific and developmental regulation of rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs.
1988,
Pubmed
Prakriya,
Orai1 is an essential pore subunit of the CRAC channel.
2006,
Pubmed
Pu,
Distinct regulatory effects of the Na,K-ATPase gamma subunit.
2002,
Pubmed
Root,
Embryonically expressed GABA and glutamate drive electrical activity regulating neurotransmitter specification.
2008,
Pubmed
,
Xenbase
Rowe,
The role of noradrenaline in the differentiation of amphibian embryonic neurons.
1993,
Pubmed
,
Xenbase
Rowitch,
Glial specification in the vertebrate neural tube.
2004,
Pubmed
Sann,
Neurite outgrowth and in vivo sensory innervation mediated by a Ca(V)2.2-laminin beta 2 stop signal.
2008,
Pubmed
,
Xenbase
Schubiger,
A mutation of the Drosophila sodium pump alpha subunit gene results in bang-sensitive paralysis.
1994,
Pubmed
Shimojo,
Oscillations in notch signaling regulate maintenance of neural progenitors.
2008,
Pubmed
Shoshani,
The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells.
2005,
Pubmed
Soboloff,
Orai1 and STIM reconstitute store-operated calcium channel function.
2006,
Pubmed
Spitzer,
Electrical activity in early neuronal development.
2006,
Pubmed
Srinivasan,
Interaction of voltage-gated sodium channels with the extracellular matrix molecules tenascin-C and tenascin-R.
1998,
Pubmed
Therien,
Tissue-specific distribution and modulatory role of the gamma subunit of the Na,K-ATPase.
1997,
Pubmed
Tobin,
A store-operated current (SOC) mediates oxytocin autocontrol in the developing rat hypothalamus.
2006,
Pubmed
Vagin,
The role of the beta1 subunit of the Na,K-ATPase and its glycosylation in cell-cell adhesion.
2006,
Pubmed
Vig,
CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry.
2006,
Pubmed
Yaari,
Development of two types of calcium channels in cultured mammalian hippocampal neurons.
1987,
Pubmed
Yeromin,
Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai.
2006,
Pubmed
Zhou,
The bHLH transcription factor Olig2 promotes oligodendrocyte differentiation in collaboration with Nkx2.2.
2001,
Pubmed
Zhou,
The bHLH transcription factors OLIG2 and OLIG1 couple neuronal and glial subtype specification.
2002,
Pubmed
