Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Neurosci
2005 Mar 02;259:2338-47. doi: 10.1523/JNEUROSCI.4889-04.2005.
Show Gene links
Show Anatomy links
Ca2+-dependent regulation of rho GTPases triggers turning of nerve growth cones.
Jin M
,
Guan CB
,
Jiang YA
,
Chen G
,
Zhao CT
,
Cui K
,
Song YQ
,
Wu CP
,
Poo MM
,
Yuan XB
.
???displayArticle.abstract???
Cytoplasmic Ca2+ elevation and changes in Rho GTPase activity are both known to mediate axon guidance by extracellular factors, but the causal relationship between these two events has been unclear. Here we show that direct elevation of cytoplasmic Ca2+ by extracellular application of a low concentration of ryanodine, which activated Ca2+ release from intracellular stores, upregulated Cdc42/Rac, but downregulated RhoA, in cultured cerebellar granule cells and human embryonic kidney 293T cells. Chemoattractive turning of the growth cone triggered by a gradient of ryanodine was blocked by overexpression of mutant forms of Cdc42 but not of RhoA in Xenopus spinal cord neurons. Furthermore, Ca2+-induced GTPase activity correlated with activation of protein kinase C and required a basal activity of Ca2+/calmodulin-dependent protein kinase II. Thus, Rho GTPases may mediate axon guidance by linking upstream Ca2+ signals triggered by guidance factors to downstream cytoskeletal rearrangements.
Akasaki,
Phosphoinositide 3-kinase-dependent and -independent activation of the small GTPase Rac2 in human neutrophils.
1999, Pubmed
Akasaki,
Phosphoinositide 3-kinase-dependent and -independent activation of the small GTPase Rac2 in human neutrophils.
1999,
Pubmed
Alder,
Overexpression of synaptophysin enhances neurotransmitter secretion at Xenopus neuromuscular synapses.
1995,
Pubmed
,
Xenbase
Aurandt,
The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG.
2002,
Pubmed
Cau,
A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization.
2001,
Pubmed
,
Xenbase
Chadborn,
Direct measurement of local raised subplasmalemmal calcium concentrations in growth cones advancing on an N-cadherin substrate.
2002,
Pubmed
Costello,
The Rho-family GTP exchange factor Vav is a critical transducer of T cell receptor signals to the calcium, ERK, and NF-kappaB pathways.
1999,
Pubmed
Daub,
Rac/Cdc42 and p65PAK regulate the microtubule-destabilizing protein stathmin through phosphorylation at serine 16.
2001,
Pubmed
Dickson,
Molecular mechanisms of axon guidance.
2002,
Pubmed
Dickson,
Rho GTPases in growth cone guidance.
2001,
Pubmed
Djouder,
Inhibition of calcium release-activated calcium current by Rac/Cdc42-inactivating clostridial cytotoxins in RBL cells.
2000,
Pubmed
Etienne-Manneville,
Rho GTPases in cell biology.
2002,
Pubmed
Fleming,
Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation.
1999,
Pubmed
Fleming,
Phospholipase C-gamma, protein kinase C and Ca2+/calmodulin-dependent protein kinase II are involved in platelet-derived growth factor-induced phosphorylation of Tiam1.
1998,
Pubmed
Fu,
Turning of nerve growth cones induced by the activation of protein kinase C.
1997,
Pubmed
,
Xenbase
Gomez,
Filopodial calcium transients promote substrate-dependent growth cone turning.
2001,
Pubmed
,
Xenbase
Henley,
Guiding neuronal growth cones using Ca2+ signals.
2004,
Pubmed
Hong,
Calcium signalling in the guidance of nerve growth by netrin-1.
2000,
Pubmed
,
Xenbase
Kater,
Filopodia as detectors of environmental cues: signal integration through changes in growth cone calcium levels.
1994,
Pubmed
Levchenko,
Models of eukaryotic gradient sensing: application to chemotaxis of amoebae and neutrophils.
2002,
Pubmed
Li,
Rac1 and Cdc42 but not RhoA or Rho kinase activities are required for neurite outgrowth induced by the Netrin-1 receptor DCC (deleted in colorectal cancer) in N1E-115 neuroblastoma cells.
2002,
Pubmed
Lohof,
Asymmetric modulation of cytosolic cAMP activity induces growth cone turning.
1992,
Pubmed
,
Xenbase
Mackay,
Rho GTPases.
1998,
Pubmed
Ming,
Electrical activity modulates growth cone guidance by diffusible factors.
2001,
Pubmed
,
Xenbase
Ng,
Rac GTPases control axon growth, guidance and branching.
2002,
Pubmed
Perrot,
Plexin B regulates Rho through the guanine nucleotide exchange factors leukemia-associated Rho GEF (LARG) and PDZ-RhoGEF.
2002,
Pubmed
Powell,
Protein kinase C mediates neurite guidance at an astrocyte boundary.
2001,
Pubmed
Price,
Calcium signaling regulates translocation and activation of Rac.
2003,
Pubmed
Ren,
Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton.
1999,
Pubmed
Servant,
Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.
2000,
Pubmed
Shen,
Growth-associated protein-43 is required for commissural axon guidance in the developing vertebrate nervous system.
2002,
Pubmed
Singleton,
CD44v10 interaction with Rho-kinase (ROK) activates inositol 1,4,5-triphosphate (IP3) receptor-mediated Ca2+ signaling during hyaluronan (HA)-induced endothelial cell migration.
2002,
Pubmed
Song,
Signal transduction underlying growth cone guidance by diffusible factors.
1999,
Pubmed
Song,
The cell biology of neuronal navigation.
2001,
Pubmed
Steven,
UNC-73 activates the Rac GTPase and is required for cell and growth cone migrations in C. elegans.
1998,
Pubmed
Swiercz,
Plexin-B1 directly interacts with PDZ-RhoGEF/LARG to regulate RhoA and growth cone morphology.
2002,
Pubmed
Wahl,
Ephrin-A5 induces collapse of growth cones by activating Rho and Rho kinase.
2000,
Pubmed
Wang,
P75 interacts with the Nogo receptor as a co-receptor for Nogo, MAG and OMgp.
2002,
Pubmed
Wang,
Localized synaptic actions of neurotrophin-4.
1998,
Pubmed
,
Xenbase
Wen,
A CaMKII/calcineurin switch controls the direction of Ca(2+)-dependent growth cone guidance.
2004,
Pubmed
,
Xenbase
Whitford,
Plexin signaling via off-track and rho family GTPases.
2001,
Pubmed
Wilk-Blaszczak,
The monomeric G-proteins Rac1 and/or Cdc42 are required for the inhibition of voltage-dependent calcium current by bradykinin.
1997,
Pubmed
Wong,
Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.
2001,
Pubmed
Wong,
A p75(NTR) and Nogo receptor complex mediates repulsive signaling by myelin-associated glycoprotein.
2002,
Pubmed
,
Xenbase
Yuan,
Signalling and crosstalk of Rho GTPases in mediating axon guidance.
2003,
Pubmed
,
Xenbase
Zhang,
GAP-43 mediates retinal axon interaction with lateral diencephalon cells during optic tract formation.
2000,
Pubmed
Zheng,
Turning of nerve growth cones induced by neurotransmitters.
1994,
Pubmed
,
Xenbase
Zheng,
Turning of nerve growth cones induced by localized increases in intracellular calcium ions.
2000,
Pubmed
,
Xenbase
Zheng,
Essential role of filopodia in chemotropic turning of nerve growth cone induced by a glutamate gradient.
1996,
Pubmed
,
Xenbase
Zucchi,
The sarcoplasmic reticulum Ca2+ channel/ryanodine receptor: modulation by endogenous effectors, drugs and disease states.
1997,
Pubmed
