Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Nucleic Acids Res
2001 Feb 01;293:668-76. doi: 10.1093/nar/29.3.668.
Show Gene links
Show Anatomy links
Antisense-mediated decrease in DNA ligase III expression results in reduced mitochondrial DNA integrity.
Lakshmipathy U
,
Campbell C
.
???displayArticle.abstract???
The human DNA ligase III gene encodes both nuclear and mitochondrial proteins. Abundant evidence supports the conclusion that the nuclear DNA ligase III protein plays an essential role in both base excision repair and homologous recombination. However, the role of DNA ligase III protein in mitochondrial genome dynamics has been obscure. Human tumor-derived HT1080 cells were transfected with an antisense DNA ligase III expression vector and clones with diminished levels of DNA ligase III activity identified. Mitochondrial protein extracts prepared from these clones had decreased levels of DNA ligase III relative to extracts from cells transfected with a control vector. Analysis of these clones revealed that the DNA ligase III antisense mRNA-expressing cells had reduced mtDNA content compared to control cells. In addition, the residual mtDNA present in these cells had numerous single-strand nicks that were not detected in mtDNA from control cells. Cells expressing antisense ligase III also had diminished capacity to restore their mtDNA to pre-irradiation levels following exposure to gamma-irradiation. An antisense-mediated reduction in cellular DNA ligase IV had no effect on the copy number or integrity of mtDNA. This observation, coupled with other evidence, suggests that DNA ligase IV is not present in the mitochondria and does not play a role in maintaining mtDNA integrity. We conclude that DNA ligase III is essential for the proper maintenance of mtDNA in cultured mammalian somatic cells.
Aboussekhra,
Mammalian DNA nucleotide excision repair reconstituted with purified protein components.
1995, Pubmed
Aboussekhra,
Mammalian DNA nucleotide excision repair reconstituted with purified protein components.
1995,
Pubmed
Abraham,
Modified diphenylamine reaction for increased sensitivity.
1972,
Pubmed
Anderson,
Sequence and organization of the human mitochondrial genome.
1981,
Pubmed
Applegren,
Further characterization of the human cell multiprotein DNA replication complex.
1995,
Pubmed
Bogenhagen,
Repair of mtDNA in vertebrates.
1999,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brustovetsky,
Dual responses of CNS mitochondria to elevated calcium.
2000,
Pubmed
Bryans,
Absence of DNA ligase IV protein in XR-1 cells: evidence for stabilization by XRCC4.
1999,
Pubmed
Caldecott,
An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.
1994,
Pubmed
,
Xenbase
Chen,
Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination.
1995,
Pubmed
,
Xenbase
Chu,
Double strand break repair.
1997,
Pubmed
Church,
Genomic sequencing.
1984,
Pubmed
Coffey,
Mammalian mitochondrial extracts possess DNA end-binding activity.
1999,
Pubmed
Feinberg,
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
1983,
Pubmed
Frank,
Late embryonic lethality and impaired V(D)J recombination in mice lacking DNA ligase IV.
1998,
Pubmed
Grawunder,
DNA ligase IV is essential for V(D)J recombination and DNA double-strand break repair in human precursor lymphocytes.
1998,
Pubmed
Grawunder,
Requirement for an interaction of XRCC4 with DNA ligase IV for wild-type V(D)J recombination and DNA double-strand break repair in vivo.
1998,
Pubmed
Henderson,
Cells from an immunodeficient patient (46BR) with a defect in DNA ligation are hypomutable but hypersensitive to the induction of sister chromatid exchanges.
1985,
Pubmed
Holt,
Coupled leading- and lagging-strand synthesis of mammalian mitochondrial DNA.
2000,
Pubmed
Hoy,
Recombination and ligation of transfected DNA in CHO mutant EM9, which has high levels of sister chromatid exchange.
1987,
Pubmed
Husain,
Purification and characterization of DNA ligase III from bovine testes. Homology with DNA ligase II and vaccinia DNA ligase.
1995,
Pubmed
,
Xenbase
Jessberger,
Repair of deletions and double-strand gaps by homologous recombination in a mammalian in vitro system.
1991,
Pubmed
Johnson,
The identification and purification of a novel mammalian DNA ligase.
1997,
Pubmed
Keown,
Methods for introducing DNA into mammalian cells.
1990,
Pubmed
King,
Respiratory enzymes and mitochondrial morphology of HeLa and L cells treated with chloramphenicol and ethidium bromide.
1972,
Pubmed
King,
Injection of mitochondria into human cells leads to a rapid replacement of the endogenous mitochondrial DNA.
1988,
Pubmed
King,
Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation.
1989,
Pubmed
Kubota,
Reconstitution of DNA base excision-repair with purified human proteins: interaction between DNA polymerase beta and the XRCC1 protein.
1996,
Pubmed
Labhart,
Nonhomologous DNA end joining in cell-free systems.
1999,
Pubmed
,
Xenbase
Lakshmipathy,
The human DNA ligase III gene encodes nuclear and mitochondrial proteins.
1999,
Pubmed
,
Xenbase
Lakshmipathy,
Double strand break rejoining by mammalian mitochondrial extracts.
1999,
Pubmed
LeDoux,
Repair of mitochondrial DNA after various types of DNA damage in Chinese hamster ovary cells.
1992,
Pubmed
Lehmann,
Relation between the human fibroblast strain 46BR and cell lines representative of Bloom's syndrome.
1988,
Pubmed
Levin,
A DNA ligase from mitochondria of rat liver.
1976,
Pubmed
Lönn,
Altered formation of DNA replication intermediates in human 46 BR fibroblast cells hypersensitive to 3-aminobenzamide.
1989,
Pubmed
Montecucco,
Late induction of human DNA ligase I after UV-C irradiation.
1995,
Pubmed
Pinz,
Efficient repair of abasic sites in DNA by mitochondrial enzymes.
1998,
Pubmed
,
Xenbase
Prigent,
Aberrant DNA repair and DNA replication due to an inherited enzymatic defect in human DNA ligase I.
1994,
Pubmed
Rasheed,
Characterization of a newly derived human sarcoma cell line (HT-1080).
1974,
Pubmed
Riballo,
Identification of a defect in DNA ligase IV in a radiosensitive leukaemia patient.
1999,
Pubmed
Richter,
Normal oxidative damage to mitochondrial and nuclear DNA is extensive.
1988,
Pubmed
Roberts,
Different active sites of mammalian DNA ligases I and II.
1994,
Pubmed
,
Xenbase
Schär,
A newly identified DNA ligase of Saccharomyces cerevisiae involved in RAD52-independent repair of DNA double-strand breaks.
1997,
Pubmed
Shigenaga,
Oxidative damage and mitochondrial decay in aging.
1994,
Pubmed
Skowronek,
A fluorescence-microscopic and flow-cytometric study of HeLa cells with an experimentally induced respiratory deficiency.
1992,
Pubmed
Teo,
Identification of Saccharomyces cerevisiae DNA ligase IV: involvement in DNA double-strand break repair.
1997,
Pubmed
Thacker,
The use of integrating DNA vectors to analyse the molecular defects in ionising radiation-sensitive mutants of mammalian cells including ataxia telangiectasia.
1989,
Pubmed
Tomkinson,
Location of the active site for enzyme-adenylate formation in DNA ligases.
1991,
Pubmed
Tomkinson,
Mammalian DNA ligases.
1997,
Pubmed
Turchi,
Enzymatic completion of mammalian lagging-strand DNA replication.
1994,
Pubmed
Waga,
Reconstitution of complete SV40 DNA replication with purified replication factors.
1994,
Pubmed
Wang,
Mammalian DNA ligase II is highly homologous with vaccinia DNA ligase. Identification of the DNA ligase II active site for enzyme-adenylate formation.
1994,
Pubmed
Webster,
Growth retardation and immunodeficiency in a patient with mutations in the DNA ligase I gene.
1992,
Pubmed
Wei,
Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.
1995,
Pubmed
,
Xenbase
Willer,
The yeast CDC9 gene encodes both a nuclear and a mitochondrial form of DNA ligase I.
1999,
Pubmed
Wilson,
Yeast DNA ligase IV mediates non-homologous DNA end joining.
1997,
Pubmed
Wiseman,
Reversible tenfod reduction in mitochondria DNA content of human cells treated with ethidium bromide.
1978,
Pubmed
Yakes,
Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA damage in human cells following oxidative stress.
1997,
Pubmed
