Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Biophys J
2014 Feb 18;1064:875-82. doi: 10.1016/j.bpj.2014.01.005.
Show Gene links
Show Anatomy links
CENP-A arrays are more condensed than canonical arrays at low ionic strength.
Geiss CP
,
Keramisanou D
,
Sekulic N
,
Scheffer MP
,
Black BE
,
Frangakis AS
.
???displayArticle.abstract???
The centromeric histone H3 variant centromeric protein A (CENP-A), whose sequence is the least conserved among all histone variants, is responsible for specifying the location of the centromere. Here, we present a comprehensive study of CENP-A nucleosome arrays by cryo-electron tomography. We see that CENP-A arrays have different biophysical properties than canonical ones under low ionic conditions, as they are more condensed with a 20% smaller average nearest-neighbor distance and a 30% higher nucleosome density. We find that CENP-A nucleosomes have a predominantly crossed DNA entry/exit site that is narrowed on average by 8°, and they have a propensity to stack face to face. We therefore propose that CENP-A induces geometric constraints at the nucleosome DNA entry/exit site to bring neighboring nucleosomes into close proximity. This specific property of CENP-A may be responsible for generating a fundamental process that contributes to increased chromatin fiber compaction that is propagated under physiological conditions to form centromeric chromatin.
Allshire,
Epigenetic regulation of centromeric chromatin: old dogs, new tricks?
2008, Pubmed
Allshire,
Epigenetic regulation of centromeric chromatin: old dogs, new tricks?
2008,
Pubmed
Aravamudhan,
The budding yeast point centromere associates with two Cse4 molecules during mitosis.
2013,
Pubmed
Bednar,
Chromatin conformation and salt-induced compaction: three-dimensional structural information from cryoelectron microscopy.
1995,
Pubmed
Black,
Structural determinants for generating centromeric chromatin.
2004,
Pubmed
Blower,
Conserved organization of centromeric chromatin in flies and humans.
2002,
Pubmed
Cleveland,
Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
2003,
Pubmed
Dalal,
Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells.
2007,
Pubmed
Dechassa,
Structure and Scm3-mediated assembly of budding yeast centromeric nucleosomes.
2011,
Pubmed
Dimitriadis,
Tetrameric organization of vertebrate centromeric nucleosomes.
2010,
Pubmed
Dyer,
Reconstitution of nucleosome core particles from recombinant histones and DNA.
2004,
Pubmed
Förster,
Classification of cryo-electron sub-tomograms using constrained correlation.
2008,
Pubmed
Frank,
Story in a sample-the potential (and limitations) of cryo-electron microscopy applied to molecular machines.
2013,
Pubmed
Furuyama,
Centromeric nucleosomes induce positive DNA supercoils.
2009,
Pubmed
Grigoryev,
Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions.
2009,
Pubmed
Guse,
In vitro centromere and kinetochore assembly on defined chromatin templates.
2011,
Pubmed
,
Xenbase
Hall,
High-resolution dynamic mapping of histone-DNA interactions in a nucleosome.
2009,
Pubmed
Hasson,
The octamer is the major form of CENP-A nucleosomes at human centromeres.
2013,
Pubmed
Henikoff,
The centromere paradox: stable inheritance with rapidly evolving DNA.
2001,
Pubmed
Huynh,
A method for the in vitro reconstitution of a defined "30 nm" chromatin fibre containing stoichiometric amounts of the linker histone.
2005,
Pubmed
Kingston,
Biophysical characterization of the centromere-specific nucleosome from budding yeast.
2011,
Pubmed
Leuba,
Linker histone tails and N-tails of histone H3 are redundant: scanning force microscopy studies of reconstituted fibers.
1998,
Pubmed
Lowary,
New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning.
1998,
Pubmed
Mangenot,
Salt-induced conformation and interaction changes of nucleosome core particles.
2002,
Pubmed
Miell,
CENP-A confers a reduction in height on octameric nucleosomes.
2013,
Pubmed
Padeganeh,
Octameric CENP-A nucleosomes are present at human centromeres throughout the cell cycle.
2013,
Pubmed
Panchenko,
Replacement of histone H3 with CENP-A directs global nucleosome array condensation and loosening of nucleosome superhelical termini.
2011,
Pubmed
Pruggnaller,
A visualization and segmentation toolbox for electron microscopy.
2008,
Pubmed
Scheffer,
Evidence for short-range helical order in the 30-nm chromatin fibers of erythrocyte nuclei.
2011,
Pubmed
Scheffer,
Nucleosomes stacked with aligned dyad axes are found in native compact chromatin in vitro.
2012,
Pubmed
Schroeder-Reiter,
Current SEM techniques for de- and re-construction of centromeres to determine 3D CENH3 distribution in barley mitotic chromosomes.
2012,
Pubmed
Schwarz,
Reversible oligonucleosome self-association: dependence on divalent cations and core histone tail domains.
1996,
Pubmed
Sekulic,
Molecular underpinnings of centromere identity and maintenance.
2012,
Pubmed
Sekulic,
The structure of (CENP-A-H4)(2) reveals physical features that mark centromeres.
2010,
Pubmed
Sullivan,
Centromeric chromatin exhibits a histone modification pattern that is distinct from both euchromatin and heterochromatin.
2004,
Pubmed
Tachiwana,
Crystal structure of the human centromeric nucleosome containing CENP-A.
2011,
Pubmed
Zheng,
Salt-dependent intra- and internucleosomal interactions of the H3 tail domain in a model oligonucleosomal array.
2005,
Pubmed
,
Xenbase
