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Cell Mol Neurobiol
1994 Jun 01;143:245-57. doi: 10.1007/bf02088323.
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Evidence that the ability to respond to a calcium stimulus in exocytosis is determined by the secretory granule membrane: comparison of exocytosis of injected bovine chromaffin granule membranes and endogenous cortical granules in Xenopus laevis oocytes.
Scheuner D
,
Holz RW
.
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1. To understand better the mechanisms which govern the sensitivity of secretory vesicles to a calcium stimulus, we compared the abilities of injected chromaffin granule membranes and of endogenous cortical granules to undergo exocytosis in Xenopus laevis oocytes and eggs in response to cytosolic Ca2+. Exocytosis of chromaffin granule membranes was detected by the appearance of dopamine-beta-hydroxylase of the chromaffin granule membrane in the oocyte or eggplasma membrane. Cortical granule exocytosis was detected by release of cortical granule lectin, a soluble constituent of cortical granules, from individual cells. 2. Injected chromaffin granule membranes undergo exocytosis equally well in frog oocytes and eggs in response to a rise in cytosolic Ca2+ induced by incubation with ionomycin. 3. Elevated Ca2+ triggered cortical granule exocytosis in eggs but not in oocytes. 4. Injected chromaffin granule membranes do not contribute factors to the oocyte that allow calcium-dependent exocytosis of the endogenous cortical granules. 5. Protein kinase C activation by phorbol esters stimulates cortical granule exocytosis in both Xenopus laevis oocytes and X. laevis eggs (Bement, W. M., and Capco, D. G., J. Cell Biol. 108, 885-892, 1989). Activation of protein kinase C by phorbol ester also stimulated chromaffin granule membrane exocytosis in oocytes, indicating that although cortical granules and chromaffin granule membranes differ in calcium responsiveness, PKC activation is an effective secretory stimulus for both. 6. These results suggest that structural or biochemical characteristics of the chromaffin granule membrane result in its ability to respond to a Ca2+ stimulus. In the oocytes, cortical granule components necessary for Ca(2+)-dependent exocytosis may be missing, nonfunctional, or unable to couple to the Ca2+ stimulus and downstream events.
Ali,
A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells.
1989, Pubmed
Ali,
A role for calpactin in calcium-dependent exocytosis in adrenal chromaffin cells.
1989,
Pubmed
Augustine,
Calcium action in synaptic transmitter release.
1987,
Pubmed
Bement,
Signal transduction by calcium and protein kinase C during egg activation.
1992,
Pubmed
Bement,
Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
1989,
Pubmed
,
Xenbase
Bement,
Transformation of the amphibian oocyte into the egg: structural and biochemical events.
1990,
Pubmed
,
Xenbase
Bittner,
Kinetic analysis of secretion from permeabilized adrenal chromaffin cells reveals distinct components.
1992,
Pubmed
Bommert,
Inhibition of neurotransmitter release by C2-domain peptides implicates synaptotagmin in exocytosis.
1993,
Pubmed
Brose,
Synaptotagmin: a calcium sensor on the synaptic vesicle surface.
1992,
Pubmed
,
Xenbase
Busa,
Activation of frog (Xenopus laevis) eggs by inositol trisphosphate. I. Characterization of Ca2+ release from intracellular stores.
1985,
Pubmed
,
Xenbase
Busa,
An elevated free cytosolic Ca2+ wave follows fertilization in eggs of the frog, Xenopus laevis.
1985,
Pubmed
,
Xenbase
Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Childs,
The steady-state kinetics of peroxidase with 2,2'-azino-di-(3-ethyl-benzthiazoline-6-sulphonic acid) as chromogen.
1975,
Pubmed
Dunn,
Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells.
1983,
Pubmed
Elferink,
A role for synaptotagmin (p65) in regulated exocytosis.
1993,
Pubmed
Elinson,
Fertilization in amphibians: the ancestry of the block to polyspermy.
1986,
Pubmed
Engvall,
Enzyme immunoassay ELISA and EMIT.
1980,
Pubmed
Grey,
Formation and structure of the fertilization envelope in Xenopus laevis.
1974,
Pubmed
,
Xenbase
Kishida,
Rab3A GTPase-activating protein-inhibiting activity of Rabphilin-3A, a putative Rab3A target protein.
1993,
Pubmed
Kubota,
Free calcium wave upon activation in Xenopus eggs.
1987,
Pubmed
,
Xenbase
Mäkinen,
Observations on the use of guaiacol and 2,2'-azino-di(3-ethylbenzthiazoline-6-sulfonic acid) as peroxidase substrates.
1982,
Pubmed
McKiernan,
The Rab3A GTPase interacts with multiple factors through the same effector domain. Mutational analysis of cross-linking of Rab3A to a putative target protein.
1993,
Pubmed
Nishihara,
Isolation and characterization of a lectin from the cortical granules of Xenopus laevis eggs.
1986,
Pubmed
,
Xenbase
Nishizuka,
Studies and perspectives of protein kinase C.
1986,
Pubmed
Ozawa,
Ca(2+)-dependent and Ca(2+)-independent isozymes of protein kinase C mediate exocytosis in antigen-stimulated rat basophilic RBL-2H3 cells. Reconstitution of secretory responses with Ca2+ and purified isozymes in washed permeabilized cells.
1993,
Pubmed
Sarafian,
The participation of annexin II (calpactin I) in calcium-evoked exocytosis requires protein kinase C.
1991,
Pubmed
Scheuner,
Bovine chromaffin granule membranes undergo Ca(2+)-regulated exocytosis in frog oocytes.
1992,
Pubmed
,
Xenbase
Shindler,
Peroxidase from human cervical mucus. The isolation and characterisation.
1976,
Pubmed
Shirataki,
Rabphilin-3A, a putative target protein for smg p25A/rab3A p25 small GTP-binding protein related to synaptotagmin.
1993,
Pubmed
Terbush,
Activation of protein kinase C is not required for exocytosis from bovine adrenal chromaffin cells. The effects of protein kinase C(19-31), Ca/CaM kinase II(291-317), and staurosporine.
1990,
Pubmed
