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Release of acetylcholine by Xenopus oocytes injected with mRNAs from cholinergic neurons.
Cavalli A
,
Eder-Colli L
,
Dunant Y
,
Loctin F
,
Morel N
.
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Xenopus laevis oocytes were injected with poly(A)+ mRNAs extracted from the electric lobes of Torpedo marmorata. The electric lobes contain the perikarya of approximately 120,000 cholinergic neurons that innervate the electric organs and are homologous to motor neurons. The injected oocytes accumulated acetylcholine and were able to synthesize [14C]acetylcholine from 1-[14C]acetate. With KCl depolarization and upon treatment with a Ca2+ ionophore, they released their endogenous as well as the radiolabelled neurotransmitter in a Ca(2+)-dependent manner. No synthesis or release were obtained from control oocytes. With respect to their dependency upon Ca2+ concentration, the oocytes injected with Torpedo electric lobe mRNAs released acetylcholine in a manner which closely resembled that found in the native synapses. In contrast to the controls, primed oocytes were also able to release [14C]acetylcholine that was injected a few hours prior to the release trial. Immunoblot analysis demonstrated that the 15 kd proteolipid antigen of the purified mediatophore, a 200 kd presynaptic protein able to translocate acetylcholine, was expressed in the ACh-releasing oocytes but not in the controls. The present observation may provide a useful approach for investigating the proteins involved in the release of acetylcholine and of other neurotransmitter substances.
Birman,
Solubilization and partial purification of a presynaptic membrane protein ensuring calcium-dependent acetylcholine release from proteoliposomes.
1986, Pubmed
Birman,
Solubilization and partial purification of a presynaptic membrane protein ensuring calcium-dependent acetylcholine release from proteoliposomes.
1986,
Pubmed
Ceccarelli,
Vesicle hypothesis of the release of quanta of acetylcholine.
1980,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Dunant,
Acetylcholine release evoked by single or a few nerve impulses in the electric organ of Torpedo.
1980,
Pubmed
Fonnum,
Choline acetyltransferase binding to and release from membranes.
1968,
Pubmed
Gaudry-Talarmain,
Effect of cetiedil analogs on acetylcholine and choline fluxes in synaptosomes and vesicles.
1989,
Pubmed
Gundersen,
Choline acetyltransferase and acetylcholine in Xenopus oocytes injected with mRNA from the electric lobe of Torpedo.
1985,
Pubmed
,
Xenbase
Israël,
Continuous determination by a chemiluminescent method of acetylcholine release and compartmentation in Torpedo electric organ synaptosomes.
1981,
Pubmed
Israël,
Purification of a presynaptic membrane protein that mediates a calcium-dependent translocation of acetylcholine.
1986,
Pubmed
Israël,
Utilization of acetate and pyruvate for the synthesis of 'total', 'bound' and 'free' acetylcholine in the electric organ of Torpedo.
1974,
Pubmed
Israël,
Reconstitution of a functional synaptosomal membrane possessing the protein constituents involved in acetylcholine translocation.
1984,
Pubmed
Jones,
Cytochrome c linked nicotinic acid hydroxylase in Pseudomonas ovalis Chester.
1973,
Pubmed
Morel,
Detection with monoclonal antibodies of a 15-kDa proteolipid in both presynaptic plasma membranes and synaptic vesicles in Torpedo electric organ.
1991,
Pubmed
Muller,
Brief occurrence of a population of presynaptic intramembrane particles coincides with transmission of a nerve impulse.
1987,
Pubmed
Muller,
Inhibition of evoked acetylcholine release: two different mechanisms in the Torpedo electric organ.
1987,
Pubmed
