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Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.
Gregan J
,
Lindner K
,
Brimage L
,
Franklin R
,
Namdar M
,
Hart EA
,
Aves SJ
,
Kearsey SE
.
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Using a cytological assay to monitor the successive chromatin association of replication proteins leading to replication initiation, we have investigated the function of fission yeast Cdc23/Mcm10 in DNA replication. Inactivation of Cdc23 before replication initiation using tight degron mutations has no effect on Mcm2 chromatin association, and thus pre-replicative complex (pre-RC) formation, although Cdc45 chromatin binding is blocked. Inactivating Cdc23 during an S phase block after Cdc45 has bound causes a small reduction in Cdc45 chromatin binding, and replication does not terminate in the absence of Mcm10 function. These observations show that Cdc23/Mcm10 function is conserved between fission yeast and Xenopus, where in vitro analysis has indicated a similar requirement for Cdc45 binding, but apparently not compared with Saccharomyces cerevisiae, where Mcm10 is needed for Mcm2 chromatin binding. However, unlike the situation in Xenopus, where Mcm10 chromatin binding is dependent on Mcm2-7, we show that the fission yeast protein is bound to chromatin throughout the cell cycle in growing cells, and only displaced from chromatin during quiescence. On return to growth, Cdc23 chromatin binding is rapidly reestablished independently from pre-RC formation, suggesting that chromatin association of Cdc23 provides a link between proliferation and competence to execute DNA replication.
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997, Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Aves,
The essential schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene.
1998,
Pubmed
Basi,
TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility.
1993,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Blow,
Replication licensing--defining the proliferative state?
2002,
Pubmed
Diffley,
The chromosome replication cycle.
2002,
Pubmed
Ding,
Global control of meiotic recombination genes by Schizosaccharomyces pombe rec16 (rep1).
1998,
Pubmed
Dohmen,
Heat-inducible degron: a method for constructing temperature-sensitive mutants.
1994,
Pubmed
Donovan,
Cdc6p-dependent loading of Mcm proteins onto pre-replicative chromatin in budding yeast.
1997,
Pubmed
,
Xenbase
Dumas,
New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication.
1982,
Pubmed
Duncker,
An N-terminal domain of Dbf4p mediates interaction with both origin recognition complex (ORC) and Rad53p and can deregulate late origin firing.
2002,
Pubmed
Edwards,
MCM2-7 complexes bind chromatin in a distributed pattern surrounding the origin recognition complex in Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Fisher,
A single fission yeast mitotic cyclin B p34cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins.
1996,
Pubmed
Grallert,
An approach to identify functional homologues and suppressors of genes in fission yeast.
1997,
Pubmed
Grallert,
A fission yeast general translation factor reveals links between protein synthesis and cell cycle controls.
2000,
Pubmed
Hart,
Fission yeast Cdc23 interactions with DNA replication initiation proteins.
2002,
Pubmed
Homesley,
Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins.
2000,
Pubmed
Ishimi,
A DNA helicase activity is associated with an MCM4, -6, and -7 protein complex.
1997,
Pubmed
Izumi,
The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase.
2000,
Pubmed
Izumi,
Cell cycle-dependent proteolysis and phosphorylation of human Mcm10.
2001,
Pubmed
Jares,
Xenopus cdc7 function is dependent on licensing but not on XORC, XCdc6, or CDK activity and is required for XCdc45 loading.
2000,
Pubmed
,
Xenbase
Kamimura,
Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae.
2001,
Pubmed
Kawasaki,
Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae.
2000,
Pubmed
Kearsey,
Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18.
2000,
Pubmed
Lee,
The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.
2003,
Pubmed
Liang,
Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.
2001,
Pubmed
Lindner,
Essential role of MCM proteins in premeiotic DNA replication.
2002,
Pubmed
Lygerou,
The fission yeast origin recognition complex is constitutively associated with chromatin and is differentially modified through the cell cycle.
1999,
Pubmed
Maine,
Mutants of S. cerevisiae defective in the maintenance of minichromosomes.
1984,
Pubmed
Maiorano,
XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis.
2000,
Pubmed
,
Xenbase
Masai,
Cdc7 kinase complex: a key regulator in the initiation of DNA replication.
2002,
Pubmed
McGarry,
Geminin, an inhibitor of DNA replication, is degraded during mitosis.
1998,
Pubmed
,
Xenbase
Méndez,
Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication.
2002,
Pubmed
Merchant,
A lesion in the DNA replication initiation factor Mcm10 induces pausing of elongation forks through chromosomal replication origins in Saccharomyces cerevisiae.
1997,
Pubmed
Mimura,
Central role for cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Mimura,
Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk.
1998,
Pubmed
,
Xenbase
Miyake,
Identification of sna41 gene, which is the suppressor of nda4 mutation and is involved in DNA replication in Schizosaccharomyces pombe.
1998,
Pubmed
Moreno,
Molecular genetic analysis of fission yeast Schizosaccharomyces pombe.
1991,
Pubmed
Nakajima,
SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.
2002,
Pubmed
Nasmyth,
Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe.
1981,
Pubmed
Nguyen,
Cyclin-dependent kinases prevent DNA re-replication through multiple mechanisms.
2001,
Pubmed
Nishitani,
The Cdt1 protein is required to license DNA for replication in fission yeast.
2000,
Pubmed
Pasero,
A role for the Cdc7 kinase regulatory subunit Dbf4p in the formation of initiation-competent origins of replication.
1999,
Pubmed
Pidoux,
Live analysis of lagging chromosomes during anaphase and their effect on spindle elongation rate in fission yeast.
2000,
Pubmed
Ritzi,
Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin.
1998,
Pubmed
Sclafani,
Cdc7p-Dbf4p becomes famous in the cell cycle.
2000,
Pubmed
Solomon,
Genetic and molecular analysis of DNA43 and DNA52: two new cell-cycle genes in Saccharomyces cerevisiae.
1992,
Pubmed
Sun,
Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatin.
2002,
Pubmed
,
Xenbase
Tada,
Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin.
2001,
Pubmed
,
Xenbase
Takeda,
Regulation of initiation of S phase, replication checkpoint signaling, and maintenance of mitotic chromosome structures during S phase by Hsk1 kinase in the fission yeast.
2001,
Pubmed
Takisawa,
Eukaryotic DNA replication: from pre-replication complex to initiation complex.
2000,
Pubmed
Tanaka,
Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs.
1997,
Pubmed
Tanaka,
Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.
1999,
Pubmed
Tercero,
DNA synthesis at individual replication forks requires the essential initiation factor Cdc45p.
2000,
Pubmed
Uchiyama,
Essential role of Sna41/Cdc45 in loading of DNA polymerase alpha onto minichromosome maintenance proteins in fission yeast.
2001,
Pubmed
,
Xenbase
Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase
Weinreich,
Cdc7p-Dbf4p kinase binds to chromatin during S phase and is regulated by both the APC and the RAD53 checkpoint pathway.
1999,
Pubmed
Wohlschlegel,
Inhibition of eukaryotic DNA replication by geminin binding to Cdt1.
2000,
Pubmed
,
Xenbase
Wohlschlegel,
Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45.
2002,
Pubmed
,
Xenbase
Zou,
Assembly of a complex containing Cdc45p, replication protein A, and Mcm2p at replication origins controlled by S-phase cyclin-dependent kinases and Cdc7p-Dbf4p kinase.
2000,
Pubmed
