Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2010 Oct 22;28543:33281-33293. doi: 10.1074/jbc.M110.102368.
Show Gene links
Show Anatomy links
Requirement for lamin B receptor and its regulation by importin {beta} and phosphorylation in nuclear envelope assembly during mitotic exit.
Lu X
,
Shi Y
,
Lu Q
,
Ma Y
,
Luo J
,
Wang Q
,
Ji J
,
Jiang Q
,
Zhang C
.
???displayArticle.abstract???
Lamin B receptor (LBR), a chromatin and lamin B-binding protein in the inner nuclear membrane, has been proposed to target the membrane precursor vesicles to chromatin mediated by importin β during the nuclear envelope (NE) assembly. However, the mechanisms for the binding of LBR with importin β and the membrane targeting by LBR in NE assembly remain largely unknown. In this report, we show that the amino acids (aa) 69-90 of LBR sequences are required to bind with importin β at aa 45-462, and the binding is essential for the NE membrane precursor vesicle targeting to the chromatin during the NE assembly at the end of mitosis. We also show that this binding is cell cycle-regulated and dependent on the phosphorylation of LBR Ser-71 by p34(cdc2) kinase. RNAi knockdown of LBR causes the NE assembly failure and abnormal chromatin decondensation of the daughter cell nuclei, leading to the daughter cell death at early G(1) phase by apoptosis. Perturbation of the interaction of LBR with importin β by deleting the LBR N-terminal spanning region or aa 69-73 also induces the NE assembly failure, the abnormal chromatin decondensation, and the daughter cell death. The first transmembrane domain of LBR promotes the NE production and expansion, because overexpressing this domain is sufficient to induce membrane overproduction of the NE. Thus, these results demonstrate that LBR targets the membrane precursor vesicles to chromatin by interacting with importin β in a LBR phosphorylation-dependent manner during the NE assembly at the end of mitosis and that the first transmembrane domain of LBR promotes the LBR-bearing membrane production and the NE expansion in interphase.
Appelbaum,
In vivo phosphorylation of the lamin B receptor. Binding of lamin B to its nuclear membrane receptor is affected by phosphorylation.
1990, Pubmed
Appelbaum,
In vivo phosphorylation of the lamin B receptor. Binding of lamin B to its nuclear membrane receptor is affected by phosphorylation.
1990,
Pubmed
Askjaer,
Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.
2002,
Pubmed
Bayliss,
GLFG and FxFG nucleoporins bind to overlapping sites on importin-beta.
2002,
Pubmed
Best,
Lamin B-receptor mutations in Pelger-Huët anomaly.
2003,
Pubmed
Chook,
Karyopherins and nuclear import.
2001,
Pubmed
Ciciarello,
Importin beta is transported to spindle poles during mitosis and regulates Ran-dependent spindle assembly factors in mammalian cells.
2004,
Pubmed
Clarke,
Ran GTPase: a master regulator of nuclear structure and function during the eukaryotic cell division cycle?
2001,
Pubmed
Clarke,
Spatial and temporal coordination of mitosis by Ran GTPase.
2008,
Pubmed
Cohen,
Functions of the nuclear envelope and lamina in development and disease.
2008,
Pubmed
Collas,
Targeting of membranes to sea urchin sperm chromatin is mediated by a lamin B receptor-like integral membrane protein.
1996,
Pubmed
Courvalin,
The lamin B receptor of the inner nuclear membrane undergoes mitosis-specific phosphorylation and is a substrate for p34cdc2-type protein kinase.
1992,
Pubmed
Delmar,
Xenopus importin beta validates human importin beta as a cell cycle negative regulator.
2008,
Pubmed
,
Xenbase
Dreger,
The nuclear envelope proteome.
2004,
Pubmed
Duband-Goulet,
LBR, a chromatin and lamin binding protein from the inner nuclear membrane, is proteolyzed at late stages of apoptosis.
1998,
Pubmed
Duband-Goulet,
Inner nuclear membrane protein LBR preferentially interacts with DNA secondary structures and nucleosomal linker.
2000,
Pubmed
Ellenberg,
Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis.
1997,
Pubmed
Ellenberg,
Dynamics and mobility of nuclear envelope proteins in interphase and mitotic cells revealed by green fluorescent protein chimeras.
1999,
Pubmed
Foisner,
Cell cycle dynamics of the nuclear envelope.
2003,
Pubmed
Forwood,
Nuclear import of Creb and AP-1 transcription factors requires importin-beta 1 and Ran but is independent of importin-alpha.
2001,
Pubmed
Gaines,
Mouse neutrophils lacking lamin B-receptor expression exhibit aberrant development and lack critical functional responses.
2008,
Pubmed
Gajewski,
Subcellular distribution of the Xenopus p58/lamin B receptor in oocytes and eggs.
1999,
Pubmed
,
Xenbase
Gant,
Nuclear assembly.
1997,
Pubmed
Gerace,
Functional organization of the nuclear envelope.
1988,
Pubmed
Goldberg,
Import and export at the nuclear envelope.
2004,
Pubmed
Goldfarb,
Importin alpha: a multipurpose nuclear-transport receptor.
2004,
Pubmed
Görlich,
Transport between the cell nucleus and the cytoplasm.
1999,
Pubmed
Guarda,
Interaction between the inner nuclear membrane lamin B receptor and the heterochromatic methyl binding protein, MeCP2.
2009,
Pubmed
Haraguchi,
Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes.
2000,
Pubmed
Harel,
Importin beta: conducting a much larger cellular symphony.
2004,
Pubmed
Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Hartl,
Nuclear assembly with lambda DNA in fractionated Xenopus egg extracts: an unexpected role for glycogen in formation of a higher order chromatin intermediate.
1994,
Pubmed
,
Xenbase
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Hetzer,
Pushing the envelope: structure, function, and dynamics of the nuclear periphery.
2005,
Pubmed
Hoffmann,
Mutations in the gene encoding the lamin B receptor produce an altered nuclear morphology in granulocytes (Pelger-Huët anomaly).
2002,
Pubmed
Hoffmann,
The granulocyte nucleus and lamin B receptor: avoiding the ovoid.
2007,
Pubmed
Hughes,
The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants.
1998,
Pubmed
,
Xenbase
Irons,
The first 238 amino acids of the human lamin B receptor are targeted to the nuclear envelope in plants.
2003,
Pubmed
Ito,
Nuclear envelope precursor vesicle targeting to chromatin is stimulated by protein phosphatase 1 in Xenopus egg extracts.
2007,
Pubmed
,
Xenbase
Kabuta,
Differential subcellular localization of insulin receptor substrates depends on C-terminal regions and importin beta.
2008,
Pubmed
Kawahire,
cDNA cloning of nuclear localization signal binding protein NBP60, a rat homologue of lamin B receptor, and identification of binding sites of human lamin B receptor for nuclear localization signals and chromatin.
1997,
Pubmed
,
Xenbase
Kushibiki,
Effect of beta-lactoglobulin on plasma retinol and triglyceride concentrations, and fatty acid composition in calves.
2001,
Pubmed
Lam,
Importin beta recognizes parathyroid hormone-related protein with high affinity and mediates its nuclear import in the absence of importin alpha.
1999,
Pubmed
Ma,
Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin beta.
2007,
Pubmed
,
Xenbase
Makatsori,
The inner nuclear membrane protein lamin B receptor forms distinct microdomains and links epigenetically marked chromatin to the nuclear envelope.
2004,
Pubmed
Marelli,
A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope.
2001,
Pubmed
Meier,
Type B lamins remain associated with the integral nuclear envelope protein p58 during mitosis: implications for nuclear reassembly.
1994,
Pubmed
Moroianu,
Distinct nuclear import and export pathways mediated by members of the karyopherin beta family.
1998,
Pubmed
Mylonis,
Temporal association of protamine 1 with the inner nuclear membrane protein lamin B receptor during spermiogenesis.
2004,
Pubmed
Nikolakaki,
A nuclear envelope-associated kinase phosphorylates arginine-serine motifs and modulates interactions between the lamin B receptor and other nuclear proteins.
1996,
Pubmed
Nikolakaki,
Mitotic phosphorylation of the lamin B receptor by a serine/arginine kinase and p34(cdc2).
1997,
Pubmed
Palmeri,
Importin beta can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin alpha.
1999,
Pubmed
Polioudaki,
Histones H3/H4 form a tight complex with the inner nuclear membrane protein LBR and heterochromatin protein 1.
2001,
Pubmed
Pyrpasopoulou,
The lamin B receptor (LBR) provides essential chromatin docking sites at the nuclear envelope.
1996,
Pubmed
Reilly,
Importin beta-mediated nuclear import of fibroblast growth factor receptor: role in cell proliferation.
2001,
Pubmed
Shah,
Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.
1998,
Pubmed
,
Xenbase
Shultz,
Mutations at the mouse ichthyosis locus are within the lamin B receptor gene: a single gene model for human Pelger-Huët anomaly.
2003,
Pubmed
Simos,
The inner nuclear membrane protein p58 associates in vivo with a p58 kinase and the nuclear lamins.
1992,
Pubmed
Smith,
The first membrane spanning region of the lamin B receptor is sufficient for sorting to the inner nuclear membrane.
1993,
Pubmed
Smith,
Colocalization of vertebrate lamin B and lamin B receptor (LBR) in nuclear envelopes and in LBR-induced membrane stacks of the yeast Saccharomyces cerevisiae.
1994,
Pubmed
Soullam,
The amino-terminal domain of the lamin B receptor is a nuclear envelope targeting signal.
1993,
Pubmed
Takano,
The binding of lamin B receptor to chromatin is regulated by phosphorylation in the RS region.
2002,
Pubmed
,
Xenbase
Takano,
Regulation of binding of lamin B receptor to chromatin by SR protein kinase and cdc2 kinase in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
Takizawa,
Ran-independent nuclear import of cyclin B1-Cdc2 by importin beta.
1999,
Pubmed
Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Waterham,
Autosomal recessive HEM/Greenberg skeletal dysplasia is caused by 3 beta-hydroxysterol delta 14-reductase deficiency due to mutations in the lamin B receptor gene.
2003,
Pubmed
Worman,
The lamin B receptor of the nuclear envelope inner membrane: a polytopic protein with eight potential transmembrane domains.
1990,
Pubmed
Ye,
Domain-specific interactions of human HP1-type chromodomain proteins and inner nuclear membrane protein LBR.
1997,
Pubmed
Ye,
Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1.
1996,
Pubmed
Ye,
Primary structure analysis and lamin B and DNA binding of human LBR, an integral protein of the nuclear envelope inner membrane.
1994,
Pubmed
Zhang,
Roles of Ran-GTP and Ran-GDP in precursor vesicle recruitment and fusion during nuclear envelope assembly in a human cell-free system.
2001,
Pubmed
,
Xenbase
Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Zhang,
Role of importin-beta in the control of nuclear envelope assembly by Ran.
2002,
Pubmed
,
Xenbase
Zhang,
Concentration of Ran on chromatin induces decondensation, nuclear envelope formation and nuclear pore complex assembly.
2002,
Pubmed
,
Xenbase
Zhang,
Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract.
1996,
Pubmed
,
Xenbase
Zwerger,
Granulocytic nuclear differentiation of lamin B receptor-deficient mouse EPRO cells.
2008,
Pubmed
