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Proc Natl Acad Sci U S A
2016 Nov 15;11346:13215-13220. doi: 10.1073/pnas.1608901113.
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Horizontal cells expressing melanopsin x are novel photoreceptors in the avian inner retina.
Morera LP
,
Díaz NM
,
Guido ME
.
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In the vertebrate retina, three types of photoreceptors-visual photoreceptor cones and rods and the intrinsically photosensitive retinal ganglion cells (ipRGCs)-converged through evolution to detect light and regulate image- and nonimage-forming activities such as photic entrainment of circadian rhythms, pupillary light reflexes, etc. ipRGCs express the nonvisual photopigment melanopsin (OPN4), encoded by two genes: the Xenopus (Opn4x) and mammalian (Opn4m) orthologs. In the chicken retina, both OPN4 proteins are found in ipRGCs, and Opn4x is also present in retinal horizontal cells (HCs), which connect with visual photoreceptors. Here we investigate the intrinsic photosensitivity and functioning of HCs from primary cultures of embryonic retinas at day 15 by using calcium fluorescent fluo4 imaging, pharmacological inhibitory treatments, and Opn4x knockdown. Results show that HCs are avian photoreceptors with a retinal-based OPN4X photopigment conferring intrinsic photosensitivity. Light responses in HCs appear to be driven through an ancient type of phototransduction cascade similar to that in rhabdomeric photoreceptors involving a G-protein q, the activation of phospholipase C, calcium mobilization, and the release of the inhibitory neurotransmitter GABA. Based on their intrinsic photosensitivity, HCs may have a key dual function in the retina of vertebrates, potentially regulating nonvisual tasks together with their sister cells, ipRGCs, and with visual photoreceptors, modulating lateral interactions and retinal processing.
Arendt,
Evolution of eyes and photoreceptor cell types.
2003, Pubmed
Arendt,
Evolution of eyes and photoreceptor cell types.
2003,
Pubmed
Bailey,
Melanopsin expression in the chick retina and pineal gland.
2005,
Pubmed
Beindl,
Inhibition of receptor/G protein coupling by suramin analogues.
1996,
Pubmed
Belecky-Adams,
Pax-6, Prox 1, and Chx10 homeobox gene expression correlates with phenotypic fate of retinal precursor cells.
1997,
Pubmed
Belgum,
Synaptic transfer of rod signals to horizontal and bipolar cells in the retina of the toad (Bufo marinus).
1988,
Pubmed
Bellingham,
Evolution of melanopsin photoreceptors: discovery and characterization of a new melanopsin in nonmammalian vertebrates.
2006,
Pubmed
,
Xenbase
Berson,
Phototransduction by retinal ganglion cells that set the circadian clock.
2002,
Pubmed
Borges,
The role of gene duplication and unconstrained selective pressures in the melanopsin gene family evolution and vertebrate circadian rhythm regulation.
2012,
Pubmed
Bultynck,
Thimerosal stimulates Ca2+ flux through inositol 1,4,5-trisphosphate receptor type 1, but not type 3, via modulation of an isoform-specific Ca2+-dependent intramolecular interaction.
2004,
Pubmed
Chaurasia,
Molecular cloning, localization and circadian expression of chicken melanopsin (Opn4): differential regulation of expression in pineal and retinal cell types.
2005,
Pubmed
,
Xenbase
Cheng,
Intrinsic light response of retinal horizontal cells of teleosts.
2009,
Pubmed
Contin,
An invertebrate-like phototransduction cascade mediates light detection in the chicken retinal ganglion cells.
2006,
Pubmed
Contín,
Light activation of the phosphoinositide cycle in intrinsically photosensitive chicken retinal ganglion cells.
2010,
Pubmed
DeVries,
Modulation of an electrical synapse between solitary pairs of catfish horizontal cells by dopamine and second messengers.
1989,
Pubmed
Díaz,
The Visual Cycle in the Inner Retina of Chicken and the Involvement of Retinal G-Protein-Coupled Receptor (RGR).
2017,
Pubmed
,
Xenbase
Díaz,
Melanopsin and the Non-visual Photochemistry in the Inner Retina of Vertebrates.
2016,
Pubmed
,
Xenbase
Doh,
Analysis of retinal cell development in chick embryo by immunohistochemistry and in ovo electroporation techniques.
2010,
Pubmed
Dong,
GABA transporters and GABAC-like receptors on catfish cone- but not rod-driven horizontal cells.
1994,
Pubmed
Drivenes,
Isolation and characterization of two teleost melanopsin genes and their differential expression within the inner retina and brain.
2003,
Pubmed
,
Xenbase
Graham,
Melanopsin ganglion cells use a membrane-associated rhabdomeric phototransduction cascade.
2008,
Pubmed
Guido,
Inner retinal circadian clocks and non-visual photoreceptors: novel players in the circadian system.
2010,
Pubmed
Hattar,
Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice.
2003,
Pubmed
Hattar,
Melanopsin-containing retinal ganglion cells: architecture, projections, and intrinsic photosensitivity.
2002,
Pubmed
Hughes,
Photic regulation of clock systems.
2015,
Pubmed
Jackman,
A positive feedback synapse from retinal horizontal cells to cone photoreceptors.
2011,
Pubmed
Jenkins,
VA opsin, melanopsin, and an inherent light response within retinal interneurons.
2003,
Pubmed
Jones,
Small-molecule antagonists of melanopsin-mediated phototransduction.
2013,
Pubmed
Kemmler,
Differential regulation of cone calcium signals by different horizontal cell feedback mechanisms in the mouse retina.
2014,
Pubmed
Kojima,
Non-visual photoreception by a variety of vertebrate opsins.
1999,
Pubmed
Kramer,
Lateral Inhibition in the Vertebrate Retina: The Case of the Missing Neurotransmitter.
2015,
Pubmed
Liu,
Calcium channels in rat horizontal cells regulate feedback inhibition of photoreceptors through an unconventional GABA- and pH-sensitive mechanism.
2013,
Pubmed
Lucas,
Diminished pupillary light reflex at high irradiances in melanopsin-knockout mice.
2003,
Pubmed
Melyan,
Addition of human melanopsin renders mammalian cells photoresponsive.
2005,
Pubmed
Morera,
A novel method to prepare highly enriched primary cultures of chicken retinal horizontal cells.
2012,
Pubmed
Panda,
Melanopsin is required for non-image-forming photic responses in blind mice.
2003,
Pubmed
Poché,
Retinal horizontal cells: challenging paradigms of neural development and cancer biology.
2009,
Pubmed
Provencio,
A novel human opsin in the inner retina.
2000,
Pubmed
Qiu,
Induction of photosensitivity by heterologous expression of melanopsin.
2005,
Pubmed
Thoreson,
Lateral interactions in the outer retina.
2012,
Pubmed
Tomonari,
Expression pattern of the melanopsin-like (cOpn4m) and VA opsin-like genes in the developing chicken retina and neural tissues.
2007,
Pubmed
Tomonari,
A non-canonical photopigment, melanopsin, is expressed in the differentiating ganglion, horizontal, and bipolar cells of the chicken retina.
2005,
Pubmed
Torii,
Two isoforms of chicken melanopsins show blue light sensitivity.
2007,
Pubmed
Vardi,
Horizontal cells in cat and monkey retina express different isoforms of glutamic acid decarboxylase.
1994,
Pubmed
Verra,
Early onset and differential temporospatial expression of melanopsin isoforms in the developing chicken retina.
2011,
Pubmed
,
Xenbase
Wu,
Modulation of synaptic gain by light.
1992,
Pubmed
Xue,
Melanopsin signalling in mammalian iris and retina.
2011,
Pubmed
