Differentiation 1976 Nov 02;71:13-21. doi: 10.1111/j.1432-0436.1977.tb01492.x.

A freeze-fracture and concanavalin A-binding study of the membrane of cleaving Xenopus embryos.

???displayArticle.abstract???
The freeze-fracture appearance and concanavalin A-binding capacity of the plasma membrane of cells of the cleaving Xenopus embryo have been examined up to the 16-cell stage. It was found that membrane on the outer surface of the embryo, which faces the vitelline membrane and is remote from cleavage furrows, and membrane in the shallow regions of the furrow possessed a high population of intramembranous particles on the PF-face (1171 per mum2). The EF-face of these membranes showed a lower particle population (245 per mum2). By contrast, membrane deep in the furrow and bounding the blastocoel did not display a face with high particle numbers. Both faces of this membrane, which is newly exposed as the furrow grows, were relatively poorly supplied with particles (93 per mum2). Therefore it appears that, in this tissue, newly added membrane possesses fewer intramembranous particles than the pre-existing membrane. Concanavalin A, as detected cytochemically using peroxidase and haemocyanin techniques, bound extensively to both particle-rich and particle-poor membrane. Thus there was no correlation between intramembranous particle frequency and degree of concanavalin A binding.

???displayArticle.pubmedLink??? 1034573
???displayArticle.link??? Differentiation