Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biochem
2000 Nov 01;2141-2:63-74. doi: 10.1023/a:1007193720930.
Show Gene links
Show Anatomy links
Evidence that the TRP-1 protein is unlikely to account for store-operated Ca2+ inflow in Xenopus laevis oocytes.
Brereton HM
,
Harland ML
,
Auld AM
,
Barritt GJ
.
???displayArticle.abstract???
The role of the TRP-1 protein, an animal cell homologue of the Drosophila transient receptor potential Ca2+ channel, in store-operated Ca2+ inflow in Xenopus laevis oocytes was investigated. A strategy involving RT-PCR and 3' and 5' rapid amplification of cDNA ends (RACE) was used to confirm and extend previous knowledge of the nucleotide and predicted amino acid sequences of Xenopus TRP-1 (xTRP-1). The predicted amino acid sequence was used to prepare an anti-TRP-l polyclonal antibody which detected the endogenous oocyte xTRP-1 protein and the human TRPC-1 protein expressed in Xenopus oocytes. Ca2+ inflow (measured using fura-2) initiated by 3-deoxy-3-fluoroinositol 1,4,5-trisphosphate (InsP3F) or lysophosphatidic acid (LPA) was completely inhibited by low concentrations of lanthanides (IC50 = 0.5 microM), indicating that InsP3F and LPA principally activate store-operated Ca2+ channels (SOCs). Antisense cRNA or antisense oligodeoxynucleotides, based on different regions of the xTRP-1 cDNA sequence, when injected into Xenopus oocytes, did not inhibit InsP3F-, LPA- or thapsigargin-stimulated Ca2+ inflow. Oocytes expressing the hTRPC-1 protein, which is 96% similar to xTRP-1, exhibited no detectable enhancement of either basal or InsP3F-stimulated Ca2+ inflow and only a very small enhancement of LPA-stimulated Ca2+ in-flow compared with control oocytes. It is concluded that the endogenous xTRP-1 protein is unlikely to be responsible for Ca2+ inflow through the previously-characterised Ca2+ -specific SOCs which are found in Xenopus oocytes. It is considered that xTRP-1 is likely to be a receptor-activated non-selective cation channel such as the channel activated by maitotoxin.
Auld,
Injection of rat hepatocyte poly(A)+ RNA to Xenopus laevis oocytes leads to expression of a constitutively-active divalent cation channel distinguishable from endogenous receptor-activated channels.
1996, Pubmed,
Xenbase
Auld,
Injection of rat hepatocyte poly(A)+ RNA to Xenopus laevis oocytes leads to expression of a constitutively-active divalent cation channel distinguishable from endogenous receptor-activated channels.
1996,
Pubmed
,
Xenbase
Barritt,
Receptor-activated Ca2+ inflow in animal cells: a variety of pathways tailored to meet different intracellular Ca2+ signalling requirements.
1999,
Pubmed
Bernard,
Maitotoxin triggers the cortical reaction and phosphatidylinositol-4,5-bisphosphate breakdown in amphibian oocytes.
1988,
Pubmed
,
Xenbase
Berridge,
Capacitative calcium entry.
1995,
Pubmed
Berridge,
Signal transduction. The calcium entry pas de deux.
2000,
Pubmed
Bielfeld-Ackermann,
Maitotoxin (MTX) activates a nonselective cation channel in Xenopus laevis oocytes.
1998,
Pubmed
,
Xenbase
Bobanovic,
Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus.
1999,
Pubmed
,
Xenbase
Bossi,
Ca2+-dependence of the depolarization-inducible Na+ current of Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Brereton,
Novel variants of voltage-operated calcium channel alpha 1-subunit transcripts in a rat liver-derived cell line: deletion in the IVS4 voltage sensing region.
1997,
Pubmed
Centinaio,
Properties of the Ca(2+)-activated Cl- current of Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Charpentier,
Induction of Na+ channel voltage sensitivity in Xenopus oocytes depends on Ca2+ mobilization.
1999,
Pubmed
,
Xenbase
Charpentier,
Phospholipase C activates protein kinase C during induction of slow Na current in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Chyb,
Polyunsaturated fatty acids activate the Drosophila light-sensitive channels TRP and TRPL.
1999,
Pubmed
Durieux,
Lysophosphatidic acid induces a pertussis toxin-sensitive Ca(2+)-activated Cl- current in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Estacion,
Stimulation of Drosophila TrpL by capacitative Ca2+ entry.
1999,
Pubmed
Finn,
Cyclic nucleotide-gated ion channels: an extended family with diverse functions.
1996,
Pubmed
Hamill,
Rapid adaptation of single mechanosensitive channels in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Harteneck,
From worm to man: three subfamilies of TRP channels.
2000,
Pubmed
Hofmann,
Transient receptor potential channels as molecular substrates of receptor-mediated cation entry.
2000,
Pubmed
Kanzaki,
Molecular identification of a eukaryotic, stretch-activated nonselective cation channel.
1999,
Pubmed
Lacerda,
T-type and N-type calcium channels of Xenopus oocytes: evidence for specific interactions with beta subunits.
1994,
Pubmed
,
Xenbase
Lan,
Expression of Drosophila trpl cRNA in Xenopus laevis oocytes leads to the appearance of a Ca2+ channel activated by Ca2+ and calmodulin, and by guanosine 5'[gamma-thio]triphosphate.
1996,
Pubmed
,
Xenbase
Lan,
The role of calmodulin-binding sites in the regulation of the Drosophila TRPL cation channel expressed in Xenopus laevis oocytes by ca2+, inositol 1,4,5-trisphosphate and GTP-binding proteins.
1998,
Pubmed
,
Xenbase
Lewis,
Store-operated calcium channels.
1999,
Pubmed
Liu,
Trp1, a candidate protein for the store-operated Ca(2+) influx mechanism in salivary gland cells.
2000,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Montell,
TRP trapped in fly signaling web.
1998,
Pubmed
Montell,
Molecular characterization of the Drosophila trp locus: a putative integral membrane protein required for phototransduction.
1989,
Pubmed
Nowatzke,
Mass spectrometric evidence that agents that cause loss of Ca2+ from intracellular compartments induce hydrolysis of arachidonic acid from pancreatic islet membrane phospholipids by a mechanism that does not require a rise in cytosolic Ca2+ concentration.
1998,
Pubmed
Parekh,
Store depletion and calcium influx.
1997,
Pubmed
Parekh,
Ca2+ oscillations and Ca2+ influx in Xenopus oocytes expressing a novel 5-hydroxytryptamine receptor.
1993,
Pubmed
,
Xenbase
Petersen,
The regulation of capacitative calcium entry by calcium and protein kinase C in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Petersen,
Putative capacitative calcium entry channels: expression of Drosophila trp and evidence for the existence of vertebrate homologues.
1995,
Pubmed
,
Xenbase
Phillips,
Identification of a Drosophila gene encoding a calmodulin-binding protein with homology to the trp phototransduction gene.
1992,
Pubmed
Putney,
Capacitative calcium entry channels.
1999,
Pubmed
Putney,
TRP, inositol 1,4,5-trisphosphate receptors, and capacitative calcium entry.
1999,
Pubmed
Rhoads,
Sequence motifs for calmodulin recognition.
1997,
Pubmed
Schilling,
Characterization of the bradykinin-stimulated calcium influx pathway of cultured vascular endothelial cells. Saturability, selectivity, and kinetics.
1989,
Pubmed
Scott,
TRP, TRPL and trouble in photoreceptor cells.
1998,
Pubmed
Sinkins,
Functional expression of TrpC1: a human homologue of the Drosophila Trp channel.
1998,
Pubmed
Taglietti,
A study of stretch-activated channels in the membrane of frog oocytes: interactions with Ca2+ ions.
1988,
Pubmed
Tomita,
Intracellular Ca2+ store-operated influx of Ca2+ through TRP-R, a rat homolog of TRP, expressed in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Wang,
Cloning of Trp1beta isoform from rat brain: immunodetection and localization of the endogenous Trp1 protein.
1999,
Pubmed
Weber,
Maitotoxin induces insertion of different ion channels into the Xenopus oocyte plasma membrane via Ca(2+)-stimulated exocytosis.
2000,
Pubmed
,
Xenbase
Weber,
Endogenous ion channels in oocytes of xenopus laevis: recent developments.
1999,
Pubmed
,
Xenbase
Weber,
Amiloride-sensitive Na+ conductance in native Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Wes,
TRPC1, a human homolog of a Drosophila store-operated channel.
1995,
Pubmed
Wu,
Functional significance of human trp1 and trp3 in store-operated Ca(2+) entry in HEK-293 cells.
2000,
Pubmed
Xu,
An enhanced chemiluminescence detection system combined with a modified immunoblot technique for the detection of low molecular weight IgM in sera from healthy adults and neonates.
1992,
Pubmed
Yang,
Characterization of stretch-activated ion channels in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Yao,
Calcium current activated by depletion of calcium stores in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Zhang,
The ion selectivity of a membrane conductance inactivated by extracellular calcium in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Zhu,
Receptor-activated Ca2+ influx via human Trp3 stably expressed in human embryonic kidney (HEK)293 cells. Evidence for a non-capacitative Ca2+ entry.
1998,
Pubmed
Zhu,
trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry.
1996,
Pubmed
Zhu,
Molecular cloning of a widely expressed human homologue for the Drosophila trp gene.
1995,
Pubmed
Zitt,
Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion.
1996,
Pubmed
