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Cell Regul
1990 Dec 01;113:989-1002. doi: 10.1091/mbc.1.13.989.
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Tyrosine phosphorylation of the gap junction protein connexin43 is required for the pp60v-src-induced inhibition of communication.
Swenson KI
,
Piwnica-Worms H
,
McNamee H
,
Paul DL
.
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Gap junction communication in some cells has been shown to be inhibited by pp60v-src, a protein tyrosine kinase encoded by the viral oncogene v-src. The gap junction protein connexin43 (Cx43) has been shown to be phosphorylated on serine in the absence of pp60v-src and on both serine and tyrosine in cells expressing pp60v-src. However, it is not known if the effect of v-src expression on communication results directly from tyrosine phosphorylation of the Cx43 or indirectly, for example, by activation of other second-messenger systems. In addition, the effect of v-src expression on communication based on other connexins has not been examined. We have used a functional expression system consisting of paired Xenopus oocytes to examine the effect of v-src expression on the regulation of communication by gap junctions comprised of different connexins. Expression of pp60v-src completely blocked the communication induced by Cx43 but had only a modest effect on communication induced by connexin32 (Cx32). Phosphoamino acid analysis showed that pp60v-src induced tyrosine phosphorylation of Cx43, but not Cx32. A mutation replacing tyrosine 265 of Cx43 with phenylalanine abolished both the inhibition of communication and the tyrosine phosphorylation induced by pp60v-src without affecting the ability of this protein to form gap junctions. These data show that the effect of pp60v-src on gap junctional communication is connexin specific and that the inhibition of Cx43-mediated junctional communication by pp60v-src requires tyrosine phosphorylation of Cx43.
Atkinson,
Rapid and reversible reduction of junctional permeability in cells infected with a temperature-sensitive mutant of avian sarcoma virus.
1981, Pubmed
Atkinson,
Rapid and reversible reduction of junctional permeability in cells infected with a temperature-sensitive mutant of avian sarcoma virus.
1981,
Pubmed
Azarnia,
The cellular src gene product regulates junctional cell-to-cell communication.
1988,
Pubmed
Azarnia,
Polyomavirus middle T antigen downregulates junctional cell-to-cell communication.
1987,
Pubmed
Azarnia,
Intercellular communication and the control of growth: X. Alteration of junctional permeability by the src gene. A study with temperature-sensitive mutant Rous sarcoma virus.
1984,
Pubmed
Beyer,
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
1987,
Pubmed
Beyer,
Antisera directed against connexin43 peptides react with a 43-kD protein localized to gap junctions in myocardium and other tissues.
1989,
Pubmed
Chang,
Potential role of the src gene product in inhibition of gap-junctional communication in NIH/3T3 cells.
1985,
Pubmed
Collett,
Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
1980,
Pubmed
Cross,
A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.
1984,
Pubmed
Crow,
Phosphorylation of connexin43 gap junction protein in uninfected and Rous sarcoma virus-transformed mammalian fibroblasts.
1990,
Pubmed
Dahl,
Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA.
1987,
Pubmed
,
Xenbase
Ebihara,
Cloning and expression of a Xenopus embryonic gap junction protein.
1989,
Pubmed
,
Xenbase
Gilmer,
Rous sarcoma virus transforming protein, p60src, expressed in E. coli, functions as a protein kinase.
1981,
Pubmed
Gimlich,
Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos.
1990,
Pubmed
,
Xenbase
Goodenough,
Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions.
1988,
Pubmed
Gould,
Platelet-derived growth factor induces multisite phosphorylation of pp60c-src and increases its protein-tyrosine kinase activity.
1988,
Pubmed
Henderson,
Structure and biochemistry of mouse hepatic gap junctions.
1979,
Pubmed
Hirst,
Phosphorylation of the fibronectin receptor complex in cells transformed by oncogenes that encode tyrosine kinases.
1986,
Pubmed
Hunter,
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
1980,
Pubmed
Hyrc,
The action of v-src on gap junctional permeability is modulated by pH.
1990,
Pubmed
Kaplan,
PDGF beta-receptor stimulates tyrosine phosphorylation of GAP and association of GAP with a signaling complex.
1990,
Pubmed
Keane,
Neural differentiation, NCAM-mediated adhesion, and gap junctional communication in neuroectoderm. A study in vitro.
1988,
Pubmed
Kumar,
Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.
1986,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Levinson,
The purified product of the transforming gene of avian sarcoma virus phosphorylates tyrosine.
1980,
Pubmed
Levitan,
Modulation of ion channels in neurons and other cells.
1988,
Pubmed
Loewenstein,
Permeability of membrane junctions.
1966,
Pubmed
Loewenstein,
Junctional intercellular communication and the control of growth.
1979,
Pubmed
Maldonado,
Growth factors modulate junctional cell-to-cell communication.
1988,
Pubmed
Mege,
Construction of epithelioid sheets by transfection of mouse sarcoma cells with cDNAs for chicken cell adhesion molecules.
1988,
Pubmed
Mehta,
Growth inhibition of transformed cells correlates with their junctional communication with normal cells.
1986,
Pubmed
Musil,
Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation.
1990,
Pubmed
Parsons,
Monoclonal antibodies to Rous sarcoma virus pp60src react with enzymatically active cellular pp60src of avian and mammalian origin.
1984,
Pubmed
Pasquale,
Talin is phosphorylated on tyrosine in chicken embryo fibroblasts transformed by Rous sarcoma virus.
1986,
Pubmed
Patschinsky,
Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
1982,
Pubmed
Paul,
Molecular cloning of cDNA for rat liver gap junction protein.
1986,
Pubmed
Piwnica-Worms,
Retrovirus shuttle vector for study of kinase activities of pp60c-src synthesized in vitro and overproduced in vivo.
1986,
Pubmed
Ralston,
The product of the protooncogene c-src is modified during the cellular response to platelet-derived growth factor.
1985,
Pubmed
Rose,
Downregulation of cell-to-cell communication by the viral src gene is blocked by TMB-8 and recovery of communication is blocked by vanadate.
1986,
Pubmed
Sefton,
Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus.
1980,
Pubmed
Sefton,
Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
1981,
Pubmed
Shriver,
Organization of pp60src and selected cytoskeletal proteins within adhesion plaques and junctions of Rous sarcoma virus-transformed rat cells.
1981,
Pubmed
Spivack,
Microinjection of pp60v-src into Xenopus oocytes increases phosphorylation of ribosomal protein S6 and accelerates the rate of progesterone-induced meiotic maturation.
1984,
Pubmed
,
Xenbase
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Swenson,
Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
1989,
Pubmed
,
Xenbase
Willingham,
Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
1979,
Pubmed
Yancey,
The 43-kD polypeptide of heart gap junctions: immunolocalization, topology, and functional domains.
1989,
Pubmed
Zhang,
Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.
1989,
Pubmed
