Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The deadenylation of maternal mRNAs in the Xenopus embryo is a sequence-specific process. One cis element that targets maternal mRNAs for deadenylation after fertilization is the embryo deadenylation element (EDEN). This element, composed of U/R repeats, is specifically bound by a protein, EDEN-BP. In the present study we show that the rate at which an RNA containing an EDEN is deadenylated can be increased by the presence of an additional cis element composed of three AUU repeats. This effect was observed for a natural EDEN (c-mos) and two synthetic EDENs. Hence, the enhancement of EDEN-dependent deadenylation conferred by the (AUU)3 motif is not due to an interaction with a particular EDEN sequence. Mutation of the (AUU)3 motif abrogated the enhancement of EDEN-dependent deadenylation. These data indicate that the rate at which a specific maternal mRNA is deadenylated in Xenopus embryos is probably defined by a cross talk between multiple cis elements.
Audic,
Design and use of easily made RNA size markers.
1997, Pubmed,
Xenbase
Audic,
Design and use of easily made RNA size markers.
1997,
Pubmed
,
Xenbase
Audic,
Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage.
1997,
Pubmed
,
Xenbase
Bouvet,
The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element.
1994,
Pubmed
,
Xenbase
Chevalier,
Cell cycle control of replication initiation in eukaryotes.
1996,
Pubmed
Fox,
Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs.
1990,
Pubmed
,
Xenbase
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Harland,
Stability of RNA in developing Xenopus embryos and identification of a destabilizing sequence in TFIIIA messenger RNA.
1988,
Pubmed
,
Xenbase
Huarte,
Transient translational silencing by reversible mRNA deadenylation.
1992,
Pubmed
Jacobson,
Interrelationships of the pathways of mRNA decay and translation in eukaryotic cells.
1996,
Pubmed
Legagneux,
Identification of RNA-binding proteins specific to Xenopus Eg maternal mRNAs: association with the portion of Eg2 mRNA that promotes deadenylation in embryos.
1992,
Pubmed
,
Xenbase
Legagneux,
Substrate-specific regulation of RNA deadenylation in Xenopus embryo and activated egg extracts.
1995,
Pubmed
,
Xenbase
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
Osborne,
Expression and post-transcriptional regulation of ornithine decarboxylase during early Xenopus development.
1991,
Pubmed
,
Xenbase
Paillard,
EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Paris,
Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis.
1988,
Pubmed
,
Xenbase
Paris,
Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development.
1990,
Pubmed
,
Xenbase
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Shyu,
Two distinct destabilizing elements in the c-fos message trigger deadenylation as a first step in rapid mRNA decay.
1991,
Pubmed
Simon,
Translational control by poly(A) elongation during Xenopus development: differential repression and enhancement by a novel cytoplasmic polyadenylation element.
1992,
Pubmed
,
Xenbase
Simon,
Further analysis of cytoplasmic polyadenylation in Xenopus embryos and identification of embryonic cytoplasmic polyadenylation element-binding proteins.
1994,
Pubmed
,
Xenbase
Stebbins-Boaz,
Translational control during early development.
1997,
Pubmed
Stebbins-Boaz,
Multiple sequence elements and a maternal mRNA product control cdk2 RNA polyadenylation and translation during early Xenopus development.
1994,
Pubmed
,
Xenbase
Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase
