Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1992 May 01;899:3999-4003.
Show Gene links
Show Anatomy links
Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae.
Woychik NA
,
Young RA
.
???displayArticle.abstract???
The gene encoding Saccharomyces cerevisiae transcription factor TFIIIA has been found adjacent to RPB6, a gene that specifies a subunit shared by nuclear RNA polymerases. Analysis of DNA upstream of the RPB6 gene revealed an open reading frame that predicts a protein, designated PZF1, with nine C2H2 zinc fingers. The presence of nine C2H2 zinc fingers in PZF1 protein, a hallmark of amphibian TFIIIA proteins, suggested that PZF1 might be a TFIIIA homologue. We found that purified recombinant PZF1 specifically binds the internal control region (ICR) of the 5S rRNA gene in S. cerevisiae. The presence of nine C2H2 zinc fingers, the specific binding to ICR DNA, and the similarity of the predicted molecular mass of PZF1 with that determined for purified yeast TFIIIA, together indicate that PZF1 is TFIIIA. The yeast and amphibian TFIIIA proteins share only a limited number of residues outside of those normally conserved in C2H2 zinc fingers; these conserved residues may provide clues to the sequence specificity of these proteins. The PZF1 gene was found to be single copy, transcribed into a 1.5-kilobase mRNA, and essential for yeast cell viability. Interestingly, the yeast RPB6 and TFIIIA coding sequences are divergently transcribed and are separated by only 233 base pairs, providing the potential for coregulated expression of components of RNA polymerases and the 5S rRNA component of ribosomes.
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985, Pubmed,
Xenbase
Bieker,
Formation of a rate-limiting intermediate in 5S RNA gene transcription.
1985,
Pubmed
,
Xenbase
Braun,
Multiple states of protein-DNA interaction in the assembly of transcription complexes on Saccharomyces cerevisiae 5S ribosomal RNA genes.
1989,
Pubmed
Brown,
The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
1985,
Pubmed
,
Xenbase
Carey,
Analysis of RNA polymerase III transcription complexes by gel filtration.
1986,
Pubmed
,
Xenbase
Challice,
Transcription of the 5 S rRNA gene of Saccharomyces cerevisiae requires a promoter element at +1 and a 14-base pair internal control region.
1989,
Pubmed
,
Xenbase
Engelke,
Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes.
1980,
Pubmed
,
Xenbase
Evans,
Zinc fingers: gilt by association.
1988,
Pubmed
Fiser-Littell,
Deletion of the N-terminal region of Xenopus transcription factor IIIA inhibits specific binding to the 5 S RNA gene.
1988,
Pubmed
,
Xenbase
Gaskins,
Sequence variation in transcription factor IIIA.
1990,
Pubmed
,
Xenbase
Geiduschek,
Transcription by RNA polymerase III.
1988,
Pubmed
Ginsberg,
Xenopus 5S gene transcription factor, TFIIIA: characterization of a cDNA clone and measurement of RNA levels throughout development.
1984,
Pubmed
,
Xenbase
Hanas,
Internal deletion mutants of Xenopus transcription factor IIIA.
1989,
Pubmed
,
Xenbase
Hartshorne,
Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIA.
,
Pubmed
,
Xenbase
Honda,
Association of a 5S gene transcription factor with 5S RNA and altered levels of the factor during cell differentiation.
1980,
Pubmed
,
Xenbase
Johnston,
Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.
1984,
Pubmed
Kadonaga,
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
1987,
Pubmed
Klevit,
Solution structure of a zinc finger domain of yeast ADR1.
1990,
Pubmed
Klevit,
Recognition of DNA by Cys2,His2 zinc fingers.
1991,
Pubmed
Kunkel,
Rapid and efficient site-specific mutagenesis without phenotypic selection.
1985,
Pubmed
Lee,
Three-dimensional solution structure of a single zinc finger DNA-binding domain.
1989,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Nardelli,
Base sequence discrimination by zinc-finger DNA-binding domains.
1991,
Pubmed
Omichinski,
High-resolution three-dimensional structure of a single zinc finger from a human enhancer binding protein in solution.
1990,
Pubmed
Párraga,
Zinc-dependent structure of a single-finger domain of yeast ADR1.
1988,
Pubmed
Pavletich,
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
1991,
Pubmed
Pelham,
A specific transcription factor that can bind either the 5S RNA gene or 5S RNA.
1980,
Pubmed
,
Xenbase
Rothstein,
One-step gene disruption in yeast.
1983,
Pubmed
Sakonju,
The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA gene.
1981,
Pubmed
,
Xenbase
Segall,
Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.
1980,
Pubmed
Setzer,
Formation and stability of the 5 S RNA transcription complex.
1985,
Pubmed
,
Xenbase
Shastry,
Multiple factors involved in the transcription of class III genes in Xenopus laevis.
1982,
Pubmed
,
Xenbase
Vrana,
Mapping functional regions of transcription factor TFIIIA.
1988,
Pubmed
,
Xenbase
Wang,
Purification and characterization of Saccharomyces cerevisiae transcription factor IIIA.
1989,
Pubmed
,
Xenbase
Woychik,
Subunits shared by eukaryotic nuclear RNA polymerases.
1990,
Pubmed
