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J Membr Biol
1994 Oct 01;1421:117-26. doi: 10.1007/bf00233389.
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Reconstitution of the influenza virus M2 ion channel in lipid bilayers.
Tosteson MT
,
Pinto LH
,
Holsinger LJ
,
Lamb RA
.
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M2, an integral membrane protein of influenza A virus, was purified from either influenza A virus-infected CV-1 cells or from Spodoptera frugiperda (Sf9) cells infected with a recombinant-M2 baculovirus. The purified protein, when incorporated into phospholipid bilayer membranes, produced ion-permeable channels with the following characteristics: (1) The channels appeared in bursts during which unit conductances of diverse magnitudes (25-500 pS) were observed. (2) The most probable open state was usually the lowest unit conductance (25-90 pS). (3) The channels were selective for cations; tNa = 0.75 when 150 mM NaCl bathed both sides of the membrane. (4) Amantadine reduced the probablity of opening of the high conductance state and also the conductance of the most probable state. (5) Reducing pH increased the mean current through the open channel as well as the conductance of the most probable state. (6) The sequence of selectivity for group IA monovalent cations was Rb > K > Cs approximately Na > Li. The pH activation, amantadine block and ion selectivity of the M2 protein ion channel in bilayers are consistent with those observed on expression of the M2 protein in oocytes of Xenopus laevis as well as for those predicted for the proposed role of an ion channel in the uncoating process of influenza virus. The finding that the M2 protein has intrinsic ion channel activity supports the hypothesis that it has ion channel activity in the influenza virus particle.
Black,
Production of the M2 protein of influenza A virus in insect cells is enhanced in the presence of amantadine.
1993, Pubmed
Black,
Production of the M2 protein of influenza A virus in insect cells is enhanced in the presence of amantadine.
1993,
Pubmed
Bron,
Role of the M2 protein in influenza virus membrane fusion: effects of amantadine and monensin on fusion kinetics.
1993,
Pubmed
Bukrinskaya,
Influenza virus uncoating in infected cells and effect of rimantadine.
1982,
Pubmed
Ciampor,
Evidence that the amantadine-induced, M2-mediated conversion of influenza A virus hemagglutinin to the low pH conformation occurs in an acidic trans Golgi compartment.
1992,
Pubmed
Ciampor,
Regulation of pH by the M2 protein of influenza A viruses.
1992,
Pubmed
Duff,
The transmembrane domain of influenza A M2 protein forms amantadine-sensitive proton channels in planar lipid bilayers.
1992,
Pubmed
Eisenberg,
The nature of the voltage-dependent conductance induced by alamethicin in black lipid membranes.
1973,
Pubmed
Eisenman,
Modeling the gramicidin channel: interpretation of experimental data using rate theory.
1984,
Pubmed
Eisenman,
Ionic selectivity revisited: the role of kinetic and equilibrium processes in ion permeation through channels.
1983,
Pubmed
Goldman,
POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES.
1943,
Pubmed
Grambas,
Maturation of influenza A virus hemagglutinin--estimates of the pH encountered during transport and its regulation by the M2 protein.
1992,
Pubmed
Grambas,
Influence of amantadine resistance mutations on the pH regulatory function of the M2 protein of influenza A viruses.
1992,
Pubmed
Hay,
The molecular basis of the specific anti-influenza action of amantadine.
1985,
Pubmed
Helenius,
Unpacking the incoming influenza virus.
1992,
Pubmed
HODGKIN,
The effect of sodium ions on the electrical activity of giant axon of the squid.
1949,
Pubmed
Holsinger,
Influenza A virus M2 ion channel protein: a structure-function analysis.
1994,
Pubmed
,
Xenbase
Holsinger,
Influenza virus M2 integral membrane protein is a homotetramer stabilized by formation of disulfide bonds.
1991,
Pubmed
Hunter,
Multi-barrelled K channels in renal tubules.
,
Pubmed
Krouse,
A large anion-selective channel has seven conductance levels.
,
Pubmed
Lamb,
Segment 8 of the influenza virus genome is unique in coding for two polypeptides.
1979,
Pubmed
Lamb,
Sequences of mRNAs derived from genome RNA segment 7 of influenza virus: colinear and interrupted mRNAs code for overlapping proteins.
1981,
Pubmed
Lamb,
Influenza virus M2 protein is an integral membrane protein expressed on the infected-cell surface.
1985,
Pubmed
Lamb,
Evidence for a ninth influenza viral polypeptide.
1978,
Pubmed
Marsh,
Keeping the viral coat on.
1992,
Pubmed
Martin,
Nuclear transport of influenza virus ribonucleoproteins: the viral matrix protein (M1) promotes export and inhibits import.
1991,
Pubmed
Miller,
Open-state substructure of single chloride channels from Torpedo electroplax.
1982,
Pubmed
Neher,
Local anaesthetics transiently block currents through single acetylcholine-receptor channels.
1978,
Pubmed
Panayotov,
Oligomeric organization and strain-specific proteolytic modification of the virion M2 protein of influenza A H1N1 viruses.
1992,
Pubmed
Pinto,
Influenza virus M2 protein has ion channel activity.
1992,
Pubmed
,
Xenbase
Skehel,
On the mechanism of inhibition of influenza virus replication by amantadine hydrochloride.
1978,
Pubmed
Skehel,
Influenza virus. Amantadine blocks the channel.
1992,
Pubmed
Sugrue,
Structural characteristics of the M2 protein of influenza A viruses: evidence that it forms a tetrameric channel.
1991,
Pubmed
Sugrue,
Specific structural alteration of the influenza haemagglutinin by amantadine.
1990,
Pubmed
Tosteson,
Ion channels induced in lipid bilayers by subvirion particles of the nonenveloped mammalian reoviruses.
1993,
Pubmed
Wang,
Ion channel activity of influenza A virus M2 protein: characterization of the amantadine block.
1993,
Pubmed
,
Xenbase
Wharton,
Role of virion M2 protein in influenza virus uncoating: specific reduction in the rate of membrane fusion between virus and liposomes by amantadine.
1994,
Pubmed
Wiley,
The structure and function of the hemagglutinin membrane glycoprotein of influenza virus.
1987,
Pubmed
Zebedee,
Characterization of the influenza virus M2 integral membrane protein and expression at the infected-cell surface from cloned cDNA.
1985,
Pubmed
Zebedee,
Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions.
1988,
Pubmed
