XB-ART-37702
Mol Biol Cell
2008 Jul 01;197:3097-110. doi: 10.1091/mbc.e07-11-1202.
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Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin.
Ducat D
,
Kawaguchi S
,
Liu H
,
Yates JR
,
Zheng Y
.
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To identify novel proteins important for microtubule assembly in mitosis, we have used a centrosome-based complementation assay to enrich for proteins with mitotic functions. An RNA interference (RNAi)-based screen of these proteins allowed us to uncover 13 novel mitotic regulators. We carried out in-depth analyses of one of these proteins, Pontin, which is known to have several functions in interphase, including chromatin remodeling, DNA repair, and transcription. We show that reduction of Pontin by RNAi resulted in defects in spindle assembly in Drosophila S2 cells and in several mammalian tissue culture cell lines. Further characterization of Pontin in Xenopus egg extracts demonstrates that Pontin interacts with the gamma tubulin ring complex (gamma-TuRC). Because depletion of Pontin leads to defects in the assembly and organization of microtubule arrays in egg extracts, our studies suggest that Pontin has a mitosis-specific function in regulating microtubule assembly.
???displayArticle.pubmedLink??? 18463163
???displayArticle.pmcLink??? PMC2441676
???displayArticle.link??? Mol Biol Cell
???displayArticle.grants??? [+]
GM-56312 NIGMS NIH HHS , MH-067880 NIMH NIH HHS, P41 RR-011823 NCRR NIH HHS , Howard Hughes Medical Institute , R01 MH067880 NIMH NIH HHS, R01 GM056312 NIGMS NIH HHS , P41 RR011823 NCRR NIH HHS
Species referenced: Xenopus
Genes referenced: ruvbl1
References [+] :
Bauer,
Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity.
2000, Pubmed
Bauer, Pontin52 and reptin52 function as antagonistic regulators of beta-catenin signalling activity. 2000, Pubmed
Bellosta, Myc interacts genetically with Tip48/Reptin and Tip49/Pontin to control growth and proliferation during Drosophila development. 2005, Pubmed
Bettencourt-Dias, Genome-wide survey of protein kinases required for cell cycle progression. 2004, Pubmed
Blagosklonny, Mitotic arrest and cell fate: why and how mitotic inhibition of transcription drives mutually exclusive events. 2007, Pubmed
Blower, A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly. 2005, Pubmed , Xenbase
Cao, The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis. 2003, Pubmed , Xenbase
Carazo-Salas, Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. 1999, Pubmed , Xenbase
Cho, TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage. 2001, Pubmed
Ems-McClung, Importin alpha/beta and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal. 2004, Pubmed , Xenbase
Etard, Expression of Xenopus homologs of the beta-catenin binding protein pontin52. 2000, Pubmed , Xenbase
Fielding, Integrin-linked kinase localizes to the centrosome and regulates mitotic spindle organization. 2008, Pubmed
Foley, Functional dissection of an innate immune response by a genome-wide RNAi screen. 2004, Pubmed
Gallant, Control of transcription by Pontin and Reptin. 2007, Pubmed
Gartner, The ATP-dependent helicase RUVBL1/TIP49a associates with tubulin during mitosis. 2003, Pubmed
Ghaemmaghami, Global analysis of protein expression in yeast. 2003, Pubmed
Goshima, Genes required for mitotic spindle assembly in Drosophila S2 cells. 2007, Pubmed
Gruss, Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity. 2001, Pubmed , Xenbase
Gunawardane, Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits. 2000, Pubmed
Gunawardane, Purification and reconstitution of Drosophila gamma-tubulin complexes. 2001, Pubmed
Hanson, AAA+ proteins: have engine, will work. 2005, Pubmed
Huang, A novel role of phospho-beta-catenin in microtubule regrowth at centrosome. 2007, Pubmed
Ikura, Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis. 2000, Pubmed
Iyer, Evolutionary history and higher order classification of AAA+ ATPases. 2004, Pubmed
Jónsson, Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex. 2004, Pubmed
Kalab, Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts. 2002, Pubmed , Xenbase
Kalab, The ran GTPase regulates mitotic spindle assembly. 1999, Pubmed , Xenbase
Kaláb, Analysis of a RanGTP-regulated gradient in mitotic somatic cells. 2006, Pubmed , Xenbase
Kaplan, Identification of a role for beta-catenin in the establishment of a bipolar mitotic spindle. 2004, Pubmed , Xenbase
Kawaguchi, Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP. 2004, Pubmed
King, A well-connected and conserved nucleoplasmic helicase is required for production of box C/D and H/ACA snoRNAs and localization of snoRNP proteins. 2001, Pubmed
Kittler, Genome-scale RNAi profiling of cell division in human tissue culture cells. 2007, Pubmed
Kittler, An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division. 2004, Pubmed
Li, Coordination of chromosome alignment and mitotic progression by the chromosome-based Ran signal. 2007, Pubmed
Li, A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo. 2003, Pubmed , Xenbase
Li, Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells. 2004, Pubmed , Xenbase
Liska, Homology-based functional proteomics by mass spectrometry: application to the Xenopus microtubule-associated proteome. 2004, Pubmed , Xenbase
Martin, Xgrip109: a gamma tubulin-associated protein with an essential role in gamma tubulin ring complex (gammaTuRC) assembly and centrosome function. 1998, Pubmed , Xenbase
McKeegan, A dynamic scaffold of pre-snoRNP factors facilitates human box C/D snoRNP assembly. 2007, Pubmed
Moritz, Recruitment of the gamma-tubulin ring complex to Drosophila salt-stripped centrosome scaffolds. 1998, Pubmed , Xenbase
Murray, Cell cycle extracts. 1991, Pubmed
Nachury, Importin beta is a mitotic target of the small GTPase Ran in spindle assembly. 2001, Pubmed , Xenbase
Neuwald, AAA+: A class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes. 1999, Pubmed
Newman, Box C/D snoRNA-associated proteins: two pairs of evolutionarily ancient proteins and possible links to replication and transcription. 2000, Pubmed
O'Connell, Cooperative mechanisms of mitotic spindle formation. 2007, Pubmed
Oegema, Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules. 1999, Pubmed , Xenbase
Ohba, Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran. 1999, Pubmed , Xenbase
Parvin, The BRCA1 E3 ubiquitin ligase controls centrosome dynamics. 2006, Pubmed
Puri, Dodecameric structure and ATPase activity of the human TIP48/TIP49 complex. 2007, Pubmed
Rieder, Stuck in division or passing through: what happens when cells cannot satisfy the spindle assembly checkpoint. 2004, Pubmed
Rottbauer, Reptin and pontin antagonistically regulate heart growth in zebrafish embryos. 2002, Pubmed
Royle, Clathrin is required for the function of the mitotic spindle. 2005, Pubmed
Sauer, Proteome analysis of the human mitotic spindle. 2005, Pubmed
Sawin, Mitotic spindle assembly by two different pathways in vitro. 1991, Pubmed , Xenbase
Schnackenberg, The disassembly and reassembly of functional centrosomes in vitro. 1998, Pubmed
Schnackenberg, Reconstitution of microtubule nucleation potential in centrosomes isolated from Spisula solidissima oocytes. 2000, Pubmed , Xenbase
Sigala, Relocalization of human chromatin remodeling cofactor TIP48 in mitosis. 2005, Pubmed
Sillibourne, Chromatin remodeling proteins interact with pericentrin to regulate centrosome integrity. 2007, Pubmed
Skop, Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms. 2004, Pubmed
Stearns, In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin. 1994, Pubmed , Xenbase
Stolc, Genome-wide transcriptional analysis of flagellar regeneration in Chlamydomonas reinhardtii identifies orthologs of ciliary disease genes. 2005, Pubmed
Tabb, DTASelect and Contrast: tools for assembling and comparing protein identifications from shotgun proteomics. 2002, Pubmed
Tsai, A mitotic lamin B matrix induced by RanGTP required for spindle assembly. 2006, Pubmed , Xenbase
Tsai, Aurora A kinase-coated beads function as microtubule-organizing centers and enhance RanGTP-induced spindle assembly. 2005, Pubmed , Xenbase
Vong, Chromosome alignment and segregation regulated by ubiquitination of survivin. 2005, Pubmed , Xenbase
Washburn, Large-scale analysis of the yeast proteome by multidimensional protein identification technology. 2001, Pubmed
Watkins, Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP. 2002, Pubmed
Weiske, The histidine triad protein Hint1 interacts with Pontin and Reptin and inhibits TCF-beta-catenin-mediated transcription. 2005, Pubmed
Wiese, Role of importin-beta in coupling Ran to downstream targets in microtubule assembly. 2001, Pubmed , Xenbase
Wilde, Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran. 1999, Pubmed , Xenbase
Wong, A protein interaction map of the mitotic spindle. 2007, Pubmed
Xu, A role for PML3 in centrosome duplication and genome stability. 2005, Pubmed , Xenbase
Xue, The human SWI/SNF-B chromatin-remodeling complex is related to yeast rsc and localizes at kinetochores of mitotic chromosomes. 2000, Pubmed
Zhang, The role of Xgrip210 in gamma-tubulin ring complex assembly and centrosome recruitment. 2000, Pubmed , Xenbase
Zhang, Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts. 1999, Pubmed , Xenbase
Zheng, Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex. 1995, Pubmed , Xenbase