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The recent discovery and characterization of several proteins that purify with endogenous, bound retinoid have given rise to the suggestion that these proteins, which are abundant in retina, perform a role in transport and function of vitamin A. Immunocytochemical techniques were used to localize two retinoid-binding proteins in the retina of four species. Antisera to cellular retinal-binding protein (CRALBP) and an interphotoreceptor retinoid-binding protein (IRBP) were obtained from rabbits immunized with antigens purified from bovine retina. Antibodies from each antiserum reacted with a single component in retinal homogenates and supernatants which corresponded to the molecular weight and charge of the respective antigen (non-SDS and SDS PAGE, electrophoretic transfer to nitrocellulose, immunochemical staining). Immunocytochemistry controls were antibodies from nonimmune serum and antibodies absorbed with purified antigen. Antigens were localized on frozen-sectioned bovine, rat, monkey, and human retina using immunofluorescence and the peroxidase-antiperoxidase technique. Specific staining with anti-IRBP was found in the space that surrounds photoreceptor outer segments, with heaviest labeling in a line corresponding to the retinal pigment epithelium (RPE) apical surface. Cone outer segments were positive. Staining with anti-CRALBP was found in two cell types in all species: the RPE and the Müller glial cell. Within the RPE, labeling filled the cytoplasm and was heaviest apically, with negative nuclei. Labeling of Müller cells produced Golgi-like silhouettes with intense staining of all cytoplasmic compartments. Staining of the external limiting membrane was heavy, with labeled microvilli projecting into the interphotoreceptor space. Localization of IRBP to this space bordered by three cell types (RPE, photoreceptor, and Müller) is consistent with its proposed role in transport of retinoids among cells. Localization of CRALBP in RPE corroborates previous biochemical studies; its presence in the Müller cell suggests that this glial cell may play a hitherto unsuspected role in vitamin A metabolism in retina.
Adler,
Proteins and glycoproteins of the bovine interphotoreceptor matrix: composition and fractionation.
1982, Pubmed
Adler,
Proteins and glycoproteins of the bovine interphotoreceptor matrix: composition and fractionation.
1982,
Pubmed
Adler,
Retinol-binding proteins in bovine interphotoreceptor matrix.
1982,
Pubmed
Bok,
Transport of retinol from the blood to the retina: an autoradiographic study of the pigment epithelial cell surface receptor for plasma retinol-binding protein.
1976,
Pubmed
Bridges,
Vitamin A and the role of the pigment epithelium during bleaching and regeneration of rhodopsin in the frog eye.
1976,
Pubmed
Bunt,
Fine structure and radioautography of retinal cone outer segments in goldfish and carp.
1980,
Pubmed
Bunt,
Fine structure and radioautography of rabbit photoreceptor cells.
1978,
Pubmed
Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed
DOWLING,
Chemistry of visual adaptation in the rat.
1960,
Pubmed
Engvall,
Enzyme immunoassay ELISA and EMIT.
1980,
Pubmed
Fairbanks,
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
1971,
Pubmed
FINE,
OBSERVATIONS ON THE ROD AND CONE LAYER OF THE HUMAN RETINA. A LIGHT AND ELECTRON MICROSCOPIC STUDY.
1963,
Pubmed
Futterman,
Occurrence of 11-cis-retinal-binding protein restricted to the retina.
1977,
Pubmed
Futterman,
Occurrence of a binding protein for 11-cis-retinal in retina.
1977,
Pubmed
Giloh,
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.
1982,
Pubmed
Heller,
Interactions of plasma retinol-binding protein with its receptor. Specific binding of bovine and human retinol-binding protein to pigment epithelium cells from bovine eyes.
1975,
Pubmed
Hjelm,
Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins.
1972,
Pubmed
Hurn,
Production of reagent antibodies.
1980,
Pubmed
Kanai,
Retinol-binding protein: the transport protein for vitamin A in human plasma.
1968,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lai,
Interphotoreceptor retinol-binding proteins: possible transport vehicles between compartments of the retina.
1982,
Pubmed
Laties,
Procion yellow: a marker dye for outer segment disc patency and for rod renewal.
1976,
Pubmed
LaVail,
The interphotoreceptor matrix in rats with inherited retinal dystrophy.
1981,
Pubmed
Liou,
Vitamin A transport between retina and pigment epithelium--an interstitial protein carrying endogenous retinol (interstitial retinol-binding protein).
1982,
Pubmed
McLean,
Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy.
1974,
Pubmed
Ong,
Cellular retinoic acid-binding protein from rat testis. Purification and characterization.
1978,
Pubmed
Ong,
Cellular retinol-binding protein from rat liver. Purification and characterization.
1978,
Pubmed
Rask,
In vitro uptake of vitamin A from the retinol-binding plasma protein to mucosal epithelial cells from the monkey's small intestine.
1976,
Pubmed
Ross,
The binding protein for retinol from rat testis cytosol. Isolation and partial characterization.
1978,
Pubmed
Saari,
Cellular retinol- and retinoic acid-binding proteins of bovine retina. Purification and properties.
1978,
Pubmed
Saari,
Enzymatic reduction of 11-cis-retinal bound to cellular retinal-binding protein.
1982,
Pubmed
Saari,
Isolation of cellular retinoid-binding proteins from bovine retina with bound endogenous ligands.
1982,
Pubmed
Saari,
Identification of the endogenous retinoids associated with three cellular retinoid-binding proteins from bovine retina and retinal pigment epithelium.
1982,
Pubmed
Sarthy,
The ultrastructure of isolated glial (Müller) cells from the turtle retina.
1982,
Pubmed
Somogyi,
A note on the use of picric acid-paraformaldehyde-glutaraldehyde fixative for correlated light and electron microscopic immunocytochemistry.
1982,
Pubmed
Tamai,
The early appearance of disc shedding in the rat retina.
1979,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Uga,
Comparative study of the fine structure of retinal Müller cells in various vertebrates.
1973,
Pubmed
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
Zimmerman,
The distribution and proportions of vitamin A compounds during the visual cycle in the rat.
1974,
Pubmed
ZIMMERMAN,
Acid mucopolysaccharide in the retinal pigment epithelium and visual cell layer of the developing mouse eye.
1959,
Pubmed
