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J Cell Biol
1996 Oct 01;1352:399-414. doi: 10.1083/jcb.135.2.399.
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Opposing motor activities are required for the organization of the mammalian mitotic spindle pole.
Gaglio T
,
Saredi A
,
Bingham JB
,
Hasbani MJ
,
Gill SR
,
Schroer TA
,
Compton DA
.
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We use both in vitro and in vivo approaches to examine the roles of Eg5 (kinesin-related protein), cytoplasmic dynein, and dynactin in the organization of the microtubules and the localization of NuMA (Nu-clear protein that associates with the Mitotic Apparatus) at the polar ends of the mammalian mitotic spindle. Perturbation of the function of Eg5 through either immunodepletion from a cell free system for assembly of mitotic asters or antibody microinjection into cultured cells leads to organized astral microtubule arrays with expanded polar regions in which the minus ends of the microtubules emanate from a ring-like structure that contains NuMA. Conversely, perturbation of the function of cytoplasmic dynein or dynactin through either specific immunodepletition from the cell free system or expression of a dominant negative subunit of dynactin in cultured cells results in the complete lack of organization of microtubules and the failure to efficiently concentrate the NuMA protein despite its association with the microtubules. Simultaneous immunodepletion of these proteins from the cell free system for mitotic aster assembly indicates that the plus end-directed activity of Eg5 antagonizes the minus end-directed activity of cytoplasmic dynein and a minus end-directed activity associated with NuMA during the organization of the microtubules into a morphologic pole. Taken together, these results demonstrate that the unique organization of the minus ends of microtubules and the localization of NuMA at the polar ends of the mammalian mitotic spindle can be accomplished in a centrosome-independent manner by the opposing activities of plus end- and minus end-directed motors.
Afshar,
DNA binding and meiotic chromosomal localization of the Drosophila nod kinesin-like protein.
1995, Pubmed
Afshar,
DNA binding and meiotic chromosomal localization of the Drosophila nod kinesin-like protein.
1995,
Pubmed
Ando,
Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region.
1994,
Pubmed
Barton,
Going mobile: microtubule motors and chromosome segregation.
1996,
Pubmed
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Blangy,
Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo.
1995,
Pubmed
,
Xenbase
Blose,
10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin.
1984,
Pubmed
Burke,
A cell free system to study reassembly of the nuclear envelope at the end of mitosis.
1986,
Pubmed
Capecchi,
High efficiency transformation by direct microinjection of DNA into cultured mammalian cells.
1980,
Pubmed
Clark,
ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.
1994,
Pubmed
Cleveland,
NuMA: a protein involved in nuclear structure, spindle assembly, and nuclear re-formation.
1995,
Pubmed
Compton,
Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds.
1991,
Pubmed
Compton,
NuMA is required for the proper completion of mitosis.
1993,
Pubmed
Compton,
Mutation of the predicted p34cdc2 phosphorylation sites in NuMA impair the assembly of the mitotic spindle and block mitosis.
1995,
Pubmed
Compton,
Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis.
1992,
Pubmed
Compton,
NuMA, a nuclear protein involved in mitosis and nuclear reformation.
1994,
Pubmed
Coue,
Microtubule depolymerization promotes particle and chromosome movement in vitro.
1991,
Pubmed
Desai,
A new role for motor proteins as couplers to depolymerizing microtubules.
1995,
Pubmed
Doxsey,
Pericentrin, a highly conserved centrosome protein involved in microtubule organization.
1994,
Pubmed
,
Xenbase
Earnshaw,
Centromere and kinetochore structure.
1992,
Pubmed
Echeverri,
Molecular characterization of the 50-kD subunit of dynactin reveals function for the complex in chromosome alignment and spindle organization during mitosis.
1996,
Pubmed
Endow,
Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis.
1994,
Pubmed
Endow,
Chromosome distribution, molecular motors and the claret protein.
1993,
Pubmed
Fuller,
Force and counterforce in the mitotic spindle.
1992,
Pubmed
Gaglio,
NuMA is required for the organization of microtubules into aster-like mitotic arrays.
1995,
Pubmed
Gill,
Dynactin, a conserved, ubiquitously expressed component of an activator of vesicle motility mediated by cytoplasmic dynein.
1991,
Pubmed
Hayden,
Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt lung cells.
1990,
Pubmed
Holy,
Dynamic instability of microtubules as an efficient way to search in space.
1994,
Pubmed
Hoyt,
Cellular roles of kinesin and related proteins.
1994,
Pubmed
Hyman,
Two different microtubule-based motor activities with opposite polarities in kinetochores.
1991,
Pubmed
Hyman,
Morphogenetic properties of microtubules and mitotic spindle assembly.
1996,
Pubmed
Inoué,
Force generation by microtubule assembly/disassembly in mitosis and related movements.
1995,
Pubmed
Kallajoki,
Microinjection of a monoclonal antibody against SPN antigen, now identified by peptide sequences as the NuMA protein, induces micronuclei in PtK2 cells.
1993,
Pubmed
Kallajoki,
Ability to organize microtubules in taxol-treated mitotic PtK2 cells goes with the SPN antigen and not with the centrosome.
1992,
Pubmed
Kallajoki,
A 210 kDa nuclear matrix protein is a functional part of the mitotic spindle; a microinjection study using SPN monoclonal antibodies.
1991,
Pubmed
Karki,
Affinity chromatography demonstrates a direct binding between cytoplasmic dynein and the dynactin complex.
1995,
Pubmed
Koshland,
Polewards chromosome movement driven by microtubule depolymerization in vitro.
1988,
Pubmed
Kuriyama,
Characterization of a minus end-directed kinesin-like motor protein from cultured mammalian cells.
1995,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Li,
Mitotic forces control a cell-cycle checkpoint.
1995,
Pubmed
Lydersen,
Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell.
1980,
Pubmed
Maekawa,
Identification of a minus end-specific microtubule-associated protein located at the mitotic poles in cultured mammalian cells.
1991,
Pubmed
Mastronarde,
Interpolar spindle microtubules in PTK cells.
1993,
Pubmed
Matthies,
Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein.
1996,
Pubmed
McIntosh,
Mitosis.
1989,
Pubmed
McKim,
Chromosomal control of meiotic cell division.
1995,
Pubmed
Mitchison,
Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.
1989,
Pubmed
Mitchison,
Mitosis: basic concepts.
1989,
Pubmed
Murray,
Cell cycle. Tense spindles can relax.
1995,
Pubmed
Nicklas,
Mechanically cut mitotic spindles: clean cuts and stable microtubules.
1989,
Pubmed
Nicklas,
The motor for poleward chromosome movement in anaphase is in or near the kinetochore.
1989,
Pubmed
Nicklas,
Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint.
1995,
Pubmed
Pfarr,
Cytoplasmic dynein is localized to kinetochores during mitosis.
1990,
Pubmed
Plamann,
Cytoplasmic dynein and actin-related protein Arp1 are required for normal nuclear distribution in filamentous fungi.
1994,
Pubmed
Pluta,
The centromere: hub of chromosomal activities.
1995,
Pubmed
Rieder,
Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle.
1994,
Pubmed
Rieder,
Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells.
1990,
Pubmed
Rieder,
The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells.
1981,
Pubmed
Rieder,
Motile kinetochores and polar ejection forces dictate chromosome position on the vertebrate mitotic spindle.
1994,
Pubmed
Rieder,
Mitosis: towards a molecular understanding of chromosome behavior.
1991,
Pubmed
Saredi,
NuMA assembles into an extensive filamentous structure when expressed in the cell cytoplasm.
1996,
Pubmed
Saunders,
Kinesin-related proteins required for structural integrity of the mitotic spindle.
1992,
Pubmed
Saunders,
Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation.
1995,
Pubmed
Sawin,
Poleward microtubule flux mitotic spindles assembled in vitro.
1991,
Pubmed
,
Xenbase
Sawin,
Mitotic spindle organization by a plus-end-directed microtubule motor.
1992,
Pubmed
,
Xenbase
Sawin,
Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.
1992,
Pubmed
,
Xenbase
Schafer,
Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin.
1994,
Pubmed
Schroer,
Structure, function and regulation of cytoplasmic dynein.
1994,
Pubmed
Schroer,
New insights into the interaction of cytoplasmic dynein with the actin-related protein, Arp1.
1994,
Pubmed
Schroer,
Two activators of microtubule-based vesicle transport.
1991,
Pubmed
Schroer,
Actin-related protein 1 and cytoplasmic dynein-based motility - what's the connection?
1996,
Pubmed
Steuer,
Localization of cytoplasmic dynein to mitotic spindles and kinetochores.
1990,
Pubmed
Theurkauf,
Meiotic spindle assembly in Drosophila females: behavior of nonexchange chromosomes and the effects of mutations in the nod kinesin-like protein.
1992,
Pubmed
Tinsley,
p150Glued, the largest subunit of the dynactin complex, is nonessential in Neurospora but required for nuclear distribution.
1996,
Pubmed
Tousson,
Centrophilin: a novel mitotic spindle protein involved in microtubule nucleation.
1991,
Pubmed
Vaisberg,
Mammalian cells express three distinct dynein heavy chains that are localized to different cytoplasmic organelles.
1996,
Pubmed
Vallee,
Targeting of motor proteins.
1996,
Pubmed
Vaughan,
Cytoplasmic dynein binds dynactin through a direct interaction between the intermediate chains and p150Glued.
1995,
Pubmed
Verde,
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein.
1991,
Pubmed
,
Xenbase
Vernos,
Xklp1, a chromosomal Xenopus kinesin-like protein essential for spindle organization and chromosome positioning.
1995,
Pubmed
,
Xenbase
Vernos,
Chromosomes take the lead in spindle assembly.
1995,
Pubmed
Walczak,
Kinesin-related proteins at mitotic spindle poles: function and regulation.
1996,
Pubmed
Wang,
Chromokinesin: a DNA-binding, kinesin-like nuclear protein.
1995,
Pubmed
Waterman-Storer,
The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1).
1995,
Pubmed
Waters,
The force-producing mechanism for centrosome separation during spindle formation in vertebrates is intrinsic to each aster.
1993,
Pubmed
Yang,
The nuclear-mitotic apparatus protein is important in the establishment and maintenance of the bipolar mitotic spindle apparatus.
1992,
Pubmed
Yang,
NuMA: an unusually long coiled-coil related protein in the mammalian nucleus.
1992,
Pubmed
